A5045417867- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant Reproductive Biology
- Plant Stress Responses and Tolerance
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Plant Micronutrient Interactions and Effects
- Crop Yield and Soil Fertility
- Plant Virus Research Studies
- Transgenic Plants and Applications
- Plant-Microbe Interactions and Immunity
- GABA and Rice Research
- Bayesian Methods and Mixture Models
- Plant biochemistry and biosynthesis
- Genetic Mapping and Diversity in Plants and Animals
- RNA and protein synthesis mechanisms
- Gene expression and cancer classification
- Plant Genetic and Mutation Studies
- Plant responses to water stress
- Plant pathogens and resistance mechanisms
- Plant Disease Resistance and Genetics
- Seed Germination and Physiology
- Lipid metabolism and biosynthesis
University of Guelph
2013-2024
Cellular Research (United States)
2015-2018
Bar-Ilan University
2010
Syngenta (Canada)
2008-2009
Syngenta (United States)
2008-2009
Ministry of Agriculture
2008
McMaster University
2008
Ben-Gurion University of the Negev
2008
Agriculture Food and Rural Development
2008
Wright State University
2003
Abstract Seeds of the tung tree (Vernicia fordii) produce large quantities triacylglycerols (TAGs) containing ∼80% eleostearic acid, an unusual conjugated fatty acid. We present a comparative analysis genetic, functional, and cellular properties type 1 2 diacylglycerol acyltransferases (DGAT1 DGAT2), two unrelated enzymes that catalyze committed step in TAG biosynthesis. show both are encoded by single genes DGAT1 is expressed at similar levels various organs, whereas DGAT2 strongly induced...
Adverse environmental conditions produce endoplasmic reticulum (ER) stress in plants. In response to heat or ER agents, Arabidopsis seedlings mitigate damage by activating ER-associated transcription factors and a RNA splicing factor, IRE1b. IRE1b splices the mRNA-encoding bZIP60, basic leucine-zipper domain containing factor associated with unfolded protein bZIP60 is required for up-regulation of BINDING PROTEIN3 ( BIP3 ) stress, loss-of-function mutations point site mRNA are defective...
Plant peroxidases play a major role in lignin formation and wound healing are believed to be involved auxin catabolism defense pathogen attack. The function of the anionic peroxidase isozymes is best understood tobacco. These catalyze polymer form rigid cross-links between lignin, cellulose, extensin secondary plant cell wall. We report purification from tobacco their partial amino acid sequence. An oligonucleotide probe deduced sequence was used screen leaf cDNA library 1200-base-pair clone...
Summary Six‐Carbon (C 6 ‐) volatiles, including the aldehydes trans‐2‐hexenal, hexanal and cis‐3‐hexenal, as well their corresponding alcohols, are produced from damaged or wounded plant tissue a product of enzymatic activity hydroperoxide lyase (HPL), component lipoxygenase (LOX) pathway. Aerial treatment Arabidopsis seedlings with 10 μM concentrations trans‐2‐hexenal induces several genes known to be involved in plant’s defense response, phenylpropanoid‐related LOX Genes encoding...
This paper offers an account of the semantics count nous.I show that neither atomic/nonatomic distinction nor homogeneous/non-homogeneous is sufficient to explain semantic contrast between mass and nouns, since there are nouns which atomic, homogeneous.Starting from a version Chierchia's 1998a under domains I denotation singular derived via measure operation picks out sets non-overlapping entities as one by recoverable unit measurement.These call 'semantic atoms'.Since choice measurement may...
Plants need abundant nitrogen and phosphorus for higher yield. Improving plant genetics use efficiency would save potentially billions of dollars annually on fertilizers reduce global environmental pollution. This will require knowledge molecular regulators maintaining homeostasis these nutrients in plants. Previously, we reported that the NITROGEN LIMITATION ADAPTATION (NLA) gene is involved adaptive responses to low-nitrogen conditions Arabidopsis, where nla mutant plants display abrupt...
Nuclear pore complexes (NPCs) are vital to nuclear-cytoplasmic communication in eukaryotes. The yeast NPC-associated TREX-2 complex, also known as the Thp1-Sac3-Cdc31-Sus1 is anchored on NPC via nucleoporin Nup1, and essential for mRNA export. Here we report identification characterization of putative Arabidopsis thaliana complex its anchoring nucleoporin. Physical functional evidence support orthologs Thp1 Nup1. Of three homologs Sac3, two components, but, surprisingly, none required export...
Abstract The phytohormone auxin plays a critical role for plant growth by regulating the expression of set genes. One large auxin-responsive gene family this type is small auxin-up RNA (SAUR) genes, although their function largely unknown. rice (Oryza sativa) SAUR39 showed rapid induction transient change in different environmental factors, including auxin, nitrogen, salinity, cytokinin, and anoxia. Transgenic plants overexpressing resulted lower shoot root growth, altered morphology,...
Effective regulation of water balance in plants requires localized extracellular barriers that control and solute movement. We describe a clade five Arabidopsis thaliana ABCG half-transporters are required for synthesis an effective suberin barrier roots seed coats (ABCG2, ABCG6, ABCG20) intact pollen wall (ABCG1 ABCG16). Seed abcg2 abcg6 abcg20 triple mutant had increased permeability to tetrazolium red decreased content. The root system was more permeable salts zone complementary affected...
The major source of nitrogen for rice (Oryza sativa L.) is ammonium (NH4+). NH4+ uptake roots mainly governed by membrane transporters, with OsAMT1;1 being a prominent member the OsAMT1 gene family that known to be involved in transport plants. However, little about its involvement and subsequent effects on assimilation. This study shows constitutively expressed, nitrogen-responsive gene, protein product localized plasma membrane. Its expression level under control circadian rhythm....
Abstract Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed Seed nlp8 loss-of-function mutants not nitrate. NLP8 functions even a nitrate reductase-deficient mutant background, and requirement conserved among accessions. reduces abscisic acid levels nitrate-dependent manner directly...
ABSTRACT The overall aerial architecture of flowering plants depends on a group meristematic cells in the shoot apex. We demonstrate that Arabidopsis TERMINAL FLOWER 1 gene has unified effect rate progression apex through different developmental phases. In transgenic which ectopically express 1, both vegetative and reproductive phases are greatly extended. As consequence, these exhibit dramatic changes their morphology, producing an enlarged rosette leaves, followed by highly branched...
Peroxidases (EC 1.11.1.7) have been implicated in the responses of plants to physical stress and pathogens, as well a variety cellular processes including cell wall biosynthesis. Tissue samples from leaf, root, pith, callus Nicotiana tabacum were assayed for specific peroxidase isozymes by analytical isoelectric focusing. Each tissue type was found exhibit unique isozyme fingerprint. Root expressed all detectable tobacco plant, whereas each other tissues examined different subset these...
Summary Abundant nitrogen is required for the optimal growth and development of plants, while numerous biotic abiotic factors that consume soil frequently create a limitation condition. To cope with this, plants have evolved suite adaptive responses to limitation. However, molecular mechanism governing adaptability totally unknown because no reported mutant defines this trait. Here we isolated an Arabidopsis mutant, nla ( adaptation ), identified NLA gene as essential component in mechanism....
The conductive (net) anion permeability of human red blood cells was determined from net KCl or K2SO4 effluxes into low K+ media at high valinomycin concentrations, conditions under which the salt efflux is limited primarily by permeability. Disulfonic stilbenes, inhibitors exchange, also inhibited these conditions, but were less effective lower concentrations where primary limiting factor. Various 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS) had similar inhibitory effects on and...
Plants can survive a limiting nitrogen (N) supply by developing set of N limitation adaptive responses.However, the Arabidopsis nla (nitrogen adaptation) mutant fails to produce such responses, and cannot adapt limitation.In this study, was utilized understand further effect NLA on adaptation limitation.Grown with N, could not accumulate anthocyanins instead produced an limitationinduced early senescence phenotype.In contrast, when supplied phosphorus (Pi), mutants accumulated abundant did...
The Arabidopsis GATA transcription factor family has 30 members, the biological function of most which is poorly understood. Homozygous T-DNA insertion lines for 23 members were identified and analyzed. Genetic screening in defined growth conditions revealed one line with an altered phenotype, while other showed no obvious change. This line, SALK_001778, a second exon At5g56860 prevents expression domain. analysis mutant demonstrated that phenotypic change caused by single gene effect...
Temperatures higher than the optimum negatively affects plant growth and development. Tolerance to high temperature is a complex process that involves several pathways. Understanding this process, especially in crops such as rice, essential prepare for predicted climate changes due global warming. Here, we show OsMYB55 induced by overexpression of resulted improved under decreased negative effect on grain yield. Transcriptome analysis revealed an increase expression genes involved amino...
Allene oxide synthase (AOS; hydroperoxide dehydratase; EC 4.2.1.92) catalyzes the first step in biosynthesis of jasmonic acid from lipoxygenase-derived hydroperoxides free fatty acids. Using AOS cDNA tomato (Lycopersicon esculentum), which role wound-induced defense gene activation has been best described, we examined kinetics induction response to wounding and elicitors, parallel with that wound-inducible PIN II (proteinase inhibitor II) gene. was induced leaves by wounding, systemin,...