A5001205833- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Plant nutrient uptake and metabolism
- CRISPR and Genetic Engineering
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Virus Research Studies
- Plant-Microbe Interactions and Immunity
- Angiogenesis and VEGF in Cancer
- Sphingolipid Metabolism and Signaling
- Crystallization and Solubility Studies
- Seed Germination and Physiology
- X-ray Diffraction in Crystallography
- Combustion and flame dynamics
- Steroid Chemistry and Biochemistry
- Cell Adhesion Molecules Research
- Genomics and Phylogenetic Studies
- Gas Dynamics and Kinetic Theory
- Central Venous Catheters and Hemodialysis
- DNA and Nucleic Acid Chemistry
- Platelet Disorders and Treatments
- Plant Reproductive Biology
- Inflammatory mediators and NSAID effects
- Advanced biosensing and bioanalysis techniques
Osaki Citizen Hospital
2024
Kaneka (Japan)
2018-2023
Hokkaido Agricultural Research Center
2014-2022
Ethiopian Institute of Agricultural Research
2022
National Agriculture and Food Research Organization
2014-2019
Institute of Agrobiological Sciences
2016-2019
Takasago (Japan)
2018
University of Toronto
2008-2016
Canada Research Chairs
2016
Iwata City Hospital
2014
Glc has hormone-like functions and controls many vital processes through mostly unknown mechanisms in plants. We report here on the molecular cloning of GLUCOSE INSENSITIVE1 (GIN1) ABSCISIC ACID DEFICIENT2 (ABA2) which encodes a unique Arabidopsis short-chain dehydrogenase/reductase (SDR1) that as link between nutrient signaling plant hormone biosynthesis. SDR1 is related to SDR superfamily members involved retinoid steroid biosynthesis mammals sex determination maize. antagonizes ethylene...
Bioactive gibberellins (GAs) are phytohormones that regulate growth and development throughout the life cycle of plants. DELLA proteins conserved repressors modulate all aspects GA responses. These GA-signaling nuclear localized likely function as transcriptional regulators. Recent studies demonstrated GA, upon binding to its receptor, derepresses signaling pathway by directly targeting them for rapid degradation via ubiquitin-proteasome pathway. Therefore, elucidating events immediately...
In a wide range of plant species, seed germination is regulated antagonistically by two hormones, abscisic acid (ABA) and gibberellin (GA). the present study, we have revealed that ABA metabolism (both biosynthesis inactivation) was phytochrome-regulated in an opposite fashion to GA during photoreversible Arabidopsis. Endogenous levels were decreased irradiation with red (R) light pulse dark-imbibed seeds pre-treated far-red (FR) pulse, reduction response R inhibited phytochrome B...
Abstract The regulation of abscisic acid (ABA) biosynthesis is essential for plant responses to drought stress. In this study, we examined the tissue-specific localization ABA biosynthetic enzymes in turgid and dehydrated Arabidopsis (Arabidopsis thaliana) plants using specific antibodies against 9-cis-epoxycarotenoid dioxygenase 3 (AtNCED3), AtABA2, aldehyde oxidase (AAO3). Immunohistochemical analysis revealed that plants, AtABA2 AAO3 proteins were localized vascular parenchyma cells most...
Abstract CRISPR/Cas9 systems are nowadays applied extensively to effect genome editing in various organisms including plants. CRISPR from Prevotella and Francisella 1 (Cpf1) is a newly characterized RNA-guided endonuclease that has two distinct features as compared Cas9. First, Cpf1 utilizes thymidine-rich protospacer adjacent motif (PAM) while Cas9 prefers guanidine-rich PAM. could be used sequence-specific nuclease target AT-rich regions of had difficulty accessing. Second, generates DNA...
Abscisic acid (ABA) is an essential molecule in plant abiotic stress responses. It binds to soluble pyrabactin resistance1/PYR1-like/regulatory component of ABA receptor receptors and stabilizes them a conformation that inhibits clade A type II C protein phosphatases; this leads downstream SnRK2 kinase activation numerous cellular outputs. We previously described the synthetic naphthalene sulfonamide agonist pyrabactin, which activates seed responses but fails trigger substantial vegetative...
Abstract Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed Seed nlp8 loss-of-function mutants not nitrate. NLP8 functions even a nitrate reductase-deficient mutant background, and requirement conserved among accessions. reduces abscisic acid levels nitrate-dependent manner directly...
Abstract The CRISPR/Cas9 system is an efficient and convenient tool for genome editing in plants. Cas9 nuclease derived from Streptococcus pyogenes (Sp ) commonly used this system. Recently, Staphylococcus aureus (SaCas9)-mediated was reported human cells Arabidopsis . Because SaCas9 (1053 a.a.) smaller than SpCas9 (1368 a.a.), could have substantial advantages delivering expressing protein, especially using virus vectors. Since the protospacer adjacent motif (PAM) sequence of (5′-NNGRRT-3′)...
β-carotene (provitamin A) is an important target for biofortification of crops as a potential solution to the problem vitamin A deficiency that prevalent in developing countries. previous report showed dominant expression splicing variants Orange (Or) gene causes accumulation cauliflower curd. In this study, we focused on putative orthologue or rice, Osor, and attempt accumulate rice callus by modification Osor via genome editing using CRISPR/Cas9.CRISPR/Cas9 vectors were constructed...
Abscisic acid (ABA) is an important phytohormone that plays a critical role in seed development, dormancy, and stress tolerance. 9-cis-Epoxycarotenoid dioxygenase the key enzyme controlling ABA biosynthesis In this study, we investigated effect of ectopic expression another gene, ABA2 (or GLUCOSE INSENSITIVE 1 [GIN1]) encoding short-chain dehydrogenase/reductase Arabidopsis (Arabidopsis thaliana). We show ABA2-overexpressing transgenic plants with elevated levels exhibited germination delay...
Plant roots play important roles not only in the absorption of water and nutrients, but also stress tolerance. Previously, we identified RSOsPR10 as a root-specific pathogenesis-related (PR) protein induced by drought salt treatments rice. Transcripts proteins were strongly jasmonate (JA) ethylene (ET) precursor 1-aminocyclopropane-1-carboxylic acid (ACC), while salicylic (SA) almost completely suppressed these inductions. Immunohistochemical analyses showed that accumulated cortex cells...
Sphingosine 1-phosphate (S1P), a ligand for endothelial differentiation gene family proteins, is one of the most potent signal mediators released from activated platelets. Here, we report that S1P induces membrane ruffling human umbilical vein cells (HUVECs) via vascular growth factor receptor (VEGFR), Src tyrosine kinase(s), and CrkII adaptor protein. induced prominent phosphorylation in HUVECs, indicating was involved S1P-induced signaling pathway. blocked by pertussis toxin overexpression...
Seed germination is a result of the competition embryonic growth potential and mechanical constraint by surrounding tissues such as endosperm. To understand processes occurring in endosperm during germination, we analyzed tiling array expression data on dissected embryo from 6 24 h-imbibed Arabidopsis seeds. The genes preferentially expressed both seeds were enriched for those related to cell wall biosynthesis/modifications, flavonol biosynthesis, defense responses cellular transport. Loss...
Abstract Stress-induced abscisic acid (ABA) is mainly catabolized by ABA 8′-hydroxylase (ABA8ox), which also strictly regulates endogenous levels. Although three members of the ABA8ox gene family are conserved in rice, it not clear stressors induce expression these genes. Here, we found that OsABA8ox1 was induced cold stress within 24 h and OsABA8ox2 OsABA8ox3 were not. In contrast, ABA-inducible, but , restored germination a cyp707a1 / a2 a3 triple mutant Arabidopsis to rates comparable...
Endothelial cell migration is an essential step in vasculogenesis and angiogenesis, which receptor tyrosine kinases play a pivotal role. We investigated the mechanism by ephrin-B1 promotes membrane ruffling human aortic endothelial cells, because heralds body migration. especially focused on role of Crk adaptor protein EphB-mediated signaling. Using DsRed-tagged fluorescent time-lapse microscope, we showed that was recruited to nascent focal complex after stimulation. Furthermore, found p130...
Sphingosine-1-phosphate (S1P) is an intracellular second messenger and extracellular mediator through endothelial differentiation gene (EDG) receptors, which are a novel class of G-protein-coupled receptors. Although EDG has attracted much attention because its various roles, no selective agonists or antagonists have yet been developed. This could account for the delay in clarifying physiological roles members family. Because precise structural information on receptors not available,...
Statins exert atheroprotective effects through the induction of specific transcriptional factors in multiple organs. In endothelial cells, statin-dependent gene up-regulation is mediated by Kruppel-like factor (KLF) family transcription factors. To dissect mechanism regulation, we sought to determine molecular targets performing microarray analyses human umbilical vein cells (HUVECs) treated with pitavastatin, and KLF4 was determined be most highly induced gene. addition, it revealed that...
Platelet endothelial adhesion molecule-1 (PECAM-1) is a part of intercellular junctions and triggers intracellular signaling cascades upon homophilic binding. The domain PECAM-1 tyrosine phosphorylated engagement. However, it remains unclear which kinase phosphorylates PECAM-1. We sought to isolate kinases responsible for phosphorylation identified Fer as candidate, based on expression cloning. specifically at the immunoreceptor tyrosine-based inhibitory motif. Notably, induced SHP-2, known...
Excitation-contraction coupling in smooth muscle involves activation of myosin light chain (MLC) phosphorylation, which increases activity the actin-activated ATPase, resulting contraction. Phosphorylation MLC phosphatase (SMPP-1M) by Rho-associated kinase or endogenous SMPP-1M-associated inhibits SMPP-1M, enhancing phosphorylation and However, precise identity remains unclear. Biochemical evidence strongly supports idea that is related to human serine/threonine leucine zipper-interacting...