A5033421501- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- Seed Germination and Physiology
- Plant nutrient uptake and metabolism
- Photosynthetic Processes and Mechanisms
- Plant tissue culture and regeneration
- Plant Reproductive Biology
- Soybean genetics and cultivation
- Plant Parasitism and Resistance
- Plant responses to water stress
- Plant Genetic and Mutation Studies
- Light effects on plants
- Plant Gene Expression Analysis
- Plant Micronutrient Interactions and Effects
- Legume Nitrogen Fixing Symbiosis
- Plant-Microbe Interactions and Immunity
- Plant Physiology and Cultivation Studies
- Allelopathy and phytotoxic interactions
- Phytase and its Applications
- Cancer Research and Treatments
- Magnetic and Electromagnetic Effects
- Nitrogen and Sulfur Effects on Brassica
- Agricultural Science and Fertilization
- Gene expression and cancer classification
- Plant and Biological Electrophysiology Studies
University of Toronto
2015-2024
Plant (United States)
2006-2022
Meiji University
2007-2022
Texas Tech University
2022
University of California, Davis
2022
Huazhong Agricultural University
2022
Toronto Public Health
2021
Stem Cell Network
2018
RIKEN Center for Sustainable Resource Science
2004-2017
Foundation for Growth Science
2007-2017
Glc has hormone-like functions and controls many vital processes through mostly unknown mechanisms in plants. We report here on the molecular cloning of GLUCOSE INSENSITIVE1 (GIN1) ABSCISIC ACID DEFICIENT2 (ABA2) which encodes a unique Arabidopsis short-chain dehydrogenase/reductase (SDR1) that as link between nutrient signaling plant hormone biosynthesis. SDR1 is related to SDR superfamily members involved retinoid steroid biosynthesis mammals sex determination maize. antagonizes ethylene...
Bioactive gibberellins (GAs) are phytohormones that regulate growth and development throughout the life cycle of plants. DELLA proteins conserved repressors modulate all aspects GA responses. These GA-signaling nuclear localized likely function as transcriptional regulators. Recent studies demonstrated GA, upon binding to its receptor, derepresses signaling pathway by directly targeting them for rapid degradation via ubiquitin-proteasome pathway. Therefore, elucidating events immediately...
Although abscisic acid (ABA) is involved in a variety of plant growth and developmental processes, few genes that actually regulate the transduction ABA signal into cellular response have been identified. In an attempt to determine negative regulators signaling, we identified mutants, designated enhanced ABA3 (era3), increased sensitivity seed ABA. Biochemical molecular analyses demonstrated era3 mutants overaccumulate ABA, suggesting regulator synthesis. Subsequent genetic analysis alleles,...
Abstract Endogenous abscisic acid (ABA) levels are regulated by both biosynthesis and catabolism of the hormone. ABA 8′-hydroxylase is considered to be key catabolic enzyme in many physiological processes. We have previously identified that four members Arabidopsis (Arabidopsis thaliana) CYP707A gene family (CYP707A1 CYP707A4) encode 8′-hydroxylases, cyp707a2 mutants showed an increase dry imbibed seeds. In this study, we cyp707a1 mutant accumulated higher seeds than mutant. Expression...
To reveal the transcriptomes of Arabidopsis seed, comprehensive expression analysis was performed using ATH1 GeneChips (Affymetrix, Santa Clara, CA, USA). In dry more than 12 000 stored mRNA species were detected, including all ontological categories. Statistical revealed that promoters highly expressed genes in wild-type seeds overrepresented abscisic acid-responsive elements (ABREs) containing core motif ACGT. Although coupling element and seed-specific enhancer RY alone not prominently...
Summary We analyzed global gene expression in Arabidopsis response to various hormones and related experiments as part of the AtGenExpress project. The experimental agents included seven basic phytohormones (auxin, cytokinin, gibberellin, brassinosteroid, abscisic acid, jasmonate ethylene) their inhibitors. In addition, was investigated hormone‐related mutants during seed germination sulfate starvation. Hormone‐inducible genes were identified from hormone data. effects each relevance lists...
Plants respond and adapt to drought, cold high-salinity stresses in order survive. In this study, we applied Arabidopsis Affymetrix tiling arrays study the whole genome transcriptome under cold, ABA treatment conditions. The bioinformatic analysis using array data showed that 7,719 non-AGI transcriptional units (TUs) exist unannotated "intergenic" regions of genome. These include 1,275 181 TUs are induced downregulated, respectively, by stress or treatments. Most hypothetical...
In a wide range of plant species, seed germination is regulated antagonistically by two hormones, abscisic acid (ABA) and gibberellin (GA). the present study, we have revealed that ABA metabolism (both biosynthesis inactivation) was phytochrome-regulated in an opposite fashion to GA during photoreversible Arabidopsis. Endogenous levels were decreased irradiation with red (R) light pulse dark-imbibed seeds pre-treated far-red (FR) pulse, reduction response R inhibited phytochrome B...
Summary The cleavage of 9‐ cis ‐epoxycarotenoids to xanthoxin, catalyzed by ‐epoxycarotenoid dioxygenases, is considered be the key regulatory step abscisic acid (ABA) biosynthesis. In Arabidopsis, genes for these enzymes form a multigene family with nine members, only five which are thought involved in ABA production. contrast prominent function AtNCED3 stress responses, physiological and developmental role other dioxygenases (NCEDs) remain unknown. Our functional expression analyses have...
Abstract Suppression of seed germination at supraoptimal high temperature (thermoinhibiton) during summer is crucial for Arabidopsis (Arabidopsis thaliana) to establish vegetative and reproductive growth in appropriate seasons. Abscisic acid (ABA) gibberellins (GAs) are well known be involved control, but it remains unknown how these hormone actions (metabolism responsiveness) altered temperature. Here, we show that ABA levels imbibed seeds elevated this increase correlated with...
Seed germination is antagonistically controlled by the phytohormones gibberellic acid (GA) and abscisic (ABA). GA promotes seed enhancing proteasome-mediated destruction of RGL2 (for RGA-LIKE2), a key DELLA factor repressing germination. By contrast, ABA blocks inducing ABI5 ABA-INSENSITIVE5), basic domain/leucine zipper transcription Decreased synthesis leads to an increase in endogenous levels through stabilized RGL2, process that may involve XERICO, RING-H2 zinc finger promoting...
Abstract Abscisic acid (ABA) is a plant hormone that regulates seed dormancy and germination. Seeds undergo changes in both ABA content sensitivity during development germination response to internal external cues. Recent advances functional genomics have revealed the integral components involved metabolism (biosynthesis catabolism) perception, core signalling pathway, as well factors trigger ABA-mediated transcription. These allow for comparative studies be conducted on seeds under...
Abstract The regulation of abscisic acid (ABA) biosynthesis is essential for plant responses to drought stress. In this study, we examined the tissue-specific localization ABA biosynthetic enzymes in turgid and dehydrated Arabidopsis (Arabidopsis thaliana) plants using specific antibodies against 9-cis-epoxycarotenoid dioxygenase 3 (AtNCED3), AtABA2, aldehyde oxidase (AAO3). Immunohistochemical analysis revealed that plants, AtABA2 AAO3 proteins were localized vascular parenchyma cells most...
Abscisic acid (ABA) is an essential molecule in plant abiotic stress responses. It binds to soluble pyrabactin resistance1/PYR1-like/regulatory component of ABA receptor receptors and stabilizes them a conformation that inhibits clade A type II C protein phosphatases; this leads downstream SnRK2 kinase activation numerous cellular outputs. We previously described the synthetic naphthalene sulfonamide agonist pyrabactin, which activates seed responses but fails trigger substantial vegetative...
Plant survival depends on seed germination and progression through post-germinative developmental checkpoints. These processes are controlled by the stress phytohormone abscisic acid (ABA). ABA regulates basic leucine zipper transcriptional factor ABI5, a central hub of growth repression, while reactive nitrogen molecule nitric oxide (NO) counteracts during germination. However, molecular mechanisms which seeds sense more favourable conditions start germinating have remained elusive. Here we...
Strigolactones are host factors that stimulate seed germination of parasitic plant species such as Striga and Orobanche. This hormone is also important in shoot branching architecture photomorphogenic development. Strigolactone biosynthetic signaling mutants model systems, unlike plants, only show phenotypes under limited growth condition. To understand the roles strigolactones germination, it necessary to develop a tractable experimental system using plants Arabidopsis. Here, we report...
Abstract Seeds respond to multiple different environmental stimuli that regulate germination. Nitrate stimulates germination in many plants but how it does so remains unclear. Here we show the Arabidopsis NIN-like protein 8 (NLP8) is essential for nitrate-promoted seed Seed nlp8 loss-of-function mutants not nitrate. NLP8 functions even a nitrate reductase-deficient mutant background, and requirement conserved among accessions. reduces abscisic acid levels nitrate-dependent manner directly...
Abscisic acid (ABA) catabolism is one of the determinants endogenous ABA levels affecting numerous aspects plant growth and abiotic stress responses. The major catabolic pathway triggered by 8'-hydroxylation catalysed cytochrome P450 CYP707A family. Among four members Arabidopsis CYP707As, expression CYP707A3 was most highly induced in response to both dehydration subsequent rehydration. A T-DNA insertional cyp707a3-1 mutant contained higher turgid plants, which showed a reduced...
A rapid decrease of the plant hormone ABA under submergence is thought to be a prerequisite for enhanced elongation submerged shoots rice ( Oryza sativa L.). Here, we report that level phaseic acid (PA), an oxidized form ABA, increased with decreasing during submergence. The oxidation PA catalyzed by 8′-hydroxylase, which possibly encoded three genes OsABA8ox1 , - 2 and 3 ) in rice. 8′-hydroxylase activity was confirmed microsomes from yeast expressing OsABA8ox1. OsABA8ox1–green fluorescent...