A5066735781- Mosquito-borne diseases and control
- SARS-CoV-2 and COVID-19 Research
- Cardiac Fibrosis and Remodeling
- Monoclonal and Polyclonal Antibodies Research
- Protease and Inhibitor Mechanisms
- Dengue and Mosquito Control Research
- COVID-19 Clinical Research Studies
- Vibrio bacteria research studies
- Biotin and Related Studies
- Cardiac Imaging and Diagnostics
- Click Chemistry and Applications
- Medical Imaging Techniques and Applications
- Cell Adhesion Molecules Research
- Biomarkers in Disease Mechanisms
- Malaria Research and Control
- Salmonella and Campylobacter epidemiology
- Glycosylation and Glycoproteins Research
- HER2/EGFR in Cancer Research
- Viral Infections and Vectors
- Metastasis and carcinoma case studies
- Bacteriophages and microbial interactions
- Induction Heating and Inverter Technology
- Ultrasound and Hyperthermia Applications
- Long-Term Effects of COVID-19
- Signaling Pathways in Disease
The University of Tokyo
2012-2025
Niigata University
2022-2023
Kitasato University
2017-2022
Toyohashi University of Technology
2020
Yokohama National University
2017-2018
Tokyo University of Marine Science and Technology
2017-2018
University Radiology
2018
Shizuoka Medical Center
2004-2017
Shimizu (Japan)
1997-2017
Osaka University
2002-2015
Plasma pentraxin 3 (PTX3) levels are increased in patients with acute myocardial infarction, yet its involvement unstable angina pectoris (UAP) remains unclear. To critically evaluate the role of PTX3 UAP, a sensitive and precise measurement concentration is needed.We established high plasma ELISA assay system for detection using monoclonal antibodies. The lower limit our was 0.1 ng/mL, sensitivity far greater than current commercially available kit. samples were obtained from 162...
Synergistic transcriptional activation by different stimuli has been reported along with a diverse array of mechanisms, but the full scope these mechanisms yet to be elucidated.We present detailed investigation hypoxia-inducible factor (HIF) 1 dependent gene expression in endothelial cells which suggests importance crosstalk between peroxisome proliferator-activated receptor (PPAR) β/δ and HIF signaling axes. A migration assay shows synergistic interaction two stimuli, we identify...
Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors and commonly play an important role in the regulation of lipid homeostasis. To identify human PPARs-responsive genes, we established tetracycline-regulated hepatoblastoma cell lines that can be induced to express each PPAR investigated gene expression profiles these cells.The introduced was using various concentrations doxycycline culture media. We found subtype tightly controlled by concentration...
Abstract Purpose: Renal cell carcinoma (RCC) is one of the most drug-refractory cancers. The aim this study to discover a novel therapeutic target molecule for clear RCC (CCRCC), which accounts majority RCC. Experimental Design: Gene expression profiles 27 CCRCCs and 9 normal kidney tissues as well 15 various adult were examined by Affymetrix U133 Plus 2.0 arrays. Among 34 genes specifically up-regulated in CCRCC, overexpression Toll-like receptor 3 (TLR3) mRNA its protein was validated...
Abstract The defining property of core streptavidin (cSA) is not only its high binding affinity for biotin but also pronounced thermal and chemical stability. Although potential applications these properties including therapeutic methods have prompted much biological research, the immunogenicity this bacterial protein a key obstacle to clinical use. To end, we successfully constructed hypoimmunogenic cSA muteins in previous report. However, effects mutations on physicochemical were still...
Statins exert atheroprotective effects through the induction of specific transcriptional factors in multiple organs. In endothelial cells, statin-dependent gene up-regulation is mediated by Kruppel-like factor (KLF) family transcription factors. To dissect mechanism regulation, we sought to determine molecular targets performing microarray analyses human umbilical vein cells (HUVECs) treated with pitavastatin, and KLF4 was determined be most highly induced gene. addition, it revealed that...
Abstract A key issue in the post-COVID-19 era is ongoing administration of COVID-19 vaccines. Repeated vaccination essential for preparing against currently circulating and newly emerging SARS-CoV-2 variants while enabling people to continue with daily life. Optimizing strategies crucial efficiently manage medical resources establish an effective framework. Therefore, it important quantitatively understand vaccine-induced immunity dynamics be able identify poor responders lower sustained...
Long COVID is a relatively new condition for which patients are asked to employ self-management strategies manage their symptoms. However, it can be challenging individuals with long find reliable and actionable resources. The objective of this project was develop an online tool that patient-centered, accessible, customizable meet individual needs. MyGuide (www.longCOVIDguide.ca) developed in British Columbia (BC), Canada, by team included clinicians patient partners. Site visitors answer...
To characterize Japanese encephalitis virus (JEV) strains recently prevalent in Japan, JEV surveillance was performed pigs from 2002 to 2004. Eleven new isolates were obtained and compared with previous Japan other Asian countries. All of the classified into genotype 1 by nucleotide sequence analysis E gene. Two different levels neurovirulence neuroinvasiveness, but only one difference gene, Sw/Mie/34/2004 Sw/Mie/40/2004, isolated at same farm on day. Sw/Mie/40/2004 displayed higher...
The bivalent mRNA vaccine is recommended to address coronavirus disease variants, with additional doses suggested for high-risk groups. However, the effectiveness, optimal frequency, and number of remain uncertain. In this study, we examined long-term cellular humoral immune responses following fifth administration severe acute respiratory syndrome 2 (SARS-CoV-2) in patients undergoing hemodialysis. To our knowledge, first study monitor data on immunity dynamics populations after five...
Black-pigmented anaerobic bacteria, such as Porphyromonas gingivalis and Prevotella intermedia, are amongst the predominant bacteria in periodontal pockets have been implicated diseases. To elucidate roles of gingival keratinocytes, which first cells encountered by oral diseases, human keratinocytes primary culture were stimulated with cell-surface components P. Pr. intermedia. A glycoprotein fraction from intermedia (PGP) clearly augmented release interleukin-8, granulocyte...
An inactivated form of alpha(1)-antitrypsin (AT) and LDL coelutes in gel permeation chromatography. To characterize to quantify the amount this fraction AT, a monoclonal antibody was established against chloramine T-oxidized AT named OxAT-4. OxAT-4 recognized oxidatively modified including hexylaldehyde- or 4-hydroxy-2-nonenal-modified but neither normal active nor trypsin/AT complex. Comigration apoB oxidized demonstrated by Western blotting analysis AT-LDL means anti-apoB A complex...
A methodology for designing single-flux-quantum (SFQ) flip-flops composed of both conventional (0-) and π-shifted Josephson junctions is investigated. We investigated the implementation a storage loop, which can store flux quantum indispensable to express binary logic states in superconductor circuits. As all SFQ have loops, design be applied their design. designed several 0- using methodology. The performances were quantitatively evaluated by an analog circuit simulator we developed....
Abstract To reveal waning humoral immunity after second dose BNT162b2 vaccinations in a rural Japanese community and determine factors affecting antibody titers. We aimed to report Immunoglobulin G (IgG) against the SARS-CoV-2 spike (S1) protein levels neutralizing activity large scale based cohort. Methods: Participants observational cross-sectional study received of vaccination with (Pfizer/BioNTech) were not previously infected COVID-19. Questionnaire-collected data on sex, age, adverse...
Booster vaccination reduces the incidence of severe cases and mortality related to COVID-19, with cellular immunity playing an important role. However, little is known about proportion population that has achieved after booster vaccination. Thus, we conducted a Fukushima cohort database assessed humoral in 2526 residents healthcare workers Prefecture Japan through continuous blood collection every 3 months from September 2021. We identified people induced using T-SPOT.COVID test, analyzed...
Myofibroblasts contribute to the healing of infarcted areas after myocardial infarction through proliferation, migration, and production extracellular matrix (ECM). Expression endostatin, a cleaved fragment type XVIII collagen, increases in heart tissue an experimental model. In present study, we examined effect endostatin on function myofibroblasts derived from area. The model was created by ligating left anterior descending artery rats. Two weeks operation, α-smooth muscle actin...
As we learned during the COVID-19 pandemic, vaccines are one of most important tools in infectious disease control. To date, an unprecedentedly large volume high-quality data on vaccinations have been accumulated. For preparedness future pandemics beyond COVID-19, these valuable datasets should be analyzed to best shape effective vaccination strategy. We collecting longitudinal from a community-based cohort Fukushima, Japan, that consists 2,407 individuals who underwent serum sampling two or...
We report here the identification of a novel member low-density lipoprotein receptor (the LDL receptor) family through signal sequence trap screening mouse lymphocyte cDNA library. The protein was termed receptor-related 9 (LRP9). LRP9 is type I membrane predicted to contain 696 amino acids with calculated molecular mass 74 764 Da. NH2-terminal half contains two CUB domains separated by single ligand-binding repeat. second domain followed cluster three additional repeats and transmembrane...
The effect of a variety hypoxic conditions on lipid accumulation in smooth muscle cells (SMCs) was studied an arterial wall coculture and monocultivation model.Low density lipoprotein (LDL) loaded under various levels oxygen tension. Oil red O staining rabbit human SMCs revealed that greater lower Cholesterol esters were shown to accumulate tension-dependent manner by high-performance liquid chromatographic analysis. Autoradiograms using radiolabeled LDL indicated uptake more pronounced...