A5072979871- Monoclonal and Polyclonal Antibodies Research
- Protein purification and stability
- Glycosylation and Glycoproteins Research
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Viral Infectious Diseases and Gene Expression in Insects
- Chemical Synthesis and Analysis
- Bacteriophages and microbial interactions
- RNA and protein synthesis mechanisms
- Bacterial Genetics and Biotechnology
- Click Chemistry and Applications
- Hemoglobin structure and function
- Lipid Membrane Structure and Behavior
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Antimicrobial Resistance in Staphylococcus
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Heme Oxygenase-1 and Carbon Monoxide
- T-cell and B-cell Immunology
- Toxin Mechanisms and Immunotoxins
- Hearing, Cochlea, Tinnitus, Genetics
- Protein Interaction Studies and Fluorescence Analysis
- CAR-T cell therapy research
- Wnt/β-catenin signaling in development and cancer
The University of Tokyo
2016-2025
Bioengineering Center
2024
National Institute of Biomedical Innovation, Health and Nutrition
2017-2023
Sysmex (Japan)
2022
Osaka Prefecture University
2022
Bunkyo University
2015-2022
Japan Institute for Advanced Dentistry
2022
Institute of Medical Sciences
2022
Osaka Ibaraki High School
2022
Tohoku University
2001-2021
Ig-like transcript 4 (ILT4) (also known as leukocyte receptor 2, CD85d, and LILRB2) is a cell surface expressed mainly on myelomonocytic cells, whereas ILT2 1, CD85j, LILRB1) wider range of immune cells including subsets natural killer T cells. Both ILTs contain immunoreceptor tyrosine-based inhibitory motifs in their cytoplasmic tails that inhibit cellular responses by recruiting phosphatases such SHP-1 (Src homology 2 domain containing tyrosine phosphatase 1). Although these have been...
Abstract Pore-forming toxins (PFT) are water-soluble proteins that possess the remarkable ability to self-assemble on membrane of target cells, where they form pores causing cell damage. Here, we elucidate mechanism action haemolytic protein fragaceatoxin C (FraC), a α-barrel PFT, by determining crystal structures FraC at four different stages lytic mechanism, namely state, monomeric lipid-bound form, an assembly intermediate and fully assembled transmembrane pore. The structure pore...
HLA-G is a nonclassical MHC class I (MHCI) molecule that can suppress wide range of immune responses in the maternal-fetal interface. The human inhibitory receptors leukocyte Ig-like receptor (LILR) B1 [also called LIR1, transcript 2 (ILT2), or CD85j] and LILRB2 (LIR2/ILT4/CD85d) preferentially recognize HLA-G. inherently exhibits various forms, including beta(2)-microglobulin (beta(2)m)-free disulfide-linked dimer forms. Notably, LILRB1 cannot beta(2)m-free form HLA-B27, but HLA-B27. To...
A peptide with an affinity for ZnO, selected by a phage-display system, preferentially immobilizes ZnO particles on gold-coated polypropylene plate and assists in the homogeneous assembly of 10 nm diameter nanoparticles into unique flower-like morphologies (see Figure). The is selective binding but not ZnS or Eu2O3. This combinatorial library approach may yield new peptides used to create structures via biomineralization.
HLA-G is a nonclassical major histocompatibility complex class I (MHCI) molecule, which expressed in trophoblasts and confers immunological tolerance the maternal-fetal interface by binding to leukocyte Ig-like receptors (LILRs, also called as LIR/ILT/CD85) CD8. disulfide-linked dimer form both solution at cell surface. Interestingly, MHCI formations have been involved pathogenesis T activation. The structure receptor characteristics of dimers never evaluated. Here we performed studies...
Exposure of antibodies to low pH is often unavoidable for purification and viral clearance. The conformation stability two humanized monoclonal (hIgG4-A -B) directed against different antigens a mouse antibody (mIgG1) in 0.1M citrate at acidic were studied using circular dichroism (CD), differential scanning calorimetry (DSC), sedimentation velocity. Near- far-UV CD spectra showed that exposure these 2.7-3.9 induced only limited conformational changes, although the changes greater lower pH....
Currently, polymer-based prefillable syringes are being promoted to the pharmaceutical market because they provide an increased break resistance relative traditionally used glass syringes. Despite this significant advantage, possibility that barrel material can affect oligomeric state of protein drug exists. The present study was designed compare effect different syringe materials and silicone oil lubrication on aggregation. stability a recombinant fusion protein, abatacept (Orencia), fully...
Abstract Cell-surface Fcγ receptors mediate innate and adaptive immune responses. Human receptor I (hFcγRI) binds IgGs with high affinity is the only that can effectively capture monomeric IgGs. However, molecular basis of hFcγRI’s interaction Fc has not been determined, limiting our understanding this major receptor. Here we report crystal structure a complex between hFcγRI human Fc, at 1.80 Å resolution, revealing an unique hydrophobic pocket surface perfectly suited for residue Leu235...
In dying cells, reactive oxygen species stimulate the production of a cytokine that triggers healthy neighboring cells to proliferate.
Resistance to vandetanib, a type I RET kinase inhibitor, developed in patient with metastatic lung adenocarcinoma harboring CCDC6-RET fusion that initially exhibited response treatment. The resistant tumor acquired secondary mutation resulting serine-to-phenylalanine substitution at codon 904 the activation loop of domain. S904F confers resistance vandetanib by increasing ATP affinity and autophosphorylation activity kinase. A reduced interaction drug is also observed vitro for mutant...
Delivering therapeutic antibodies into the brain across blood–brain barrier at a level is promising while challenging approach in treatment of neurological disorders. Here, we present polymeric nanomicelle (PM) system capable delivering therapeutically effective levels 3D6 antibody fragments (3D6-Fab) parenchyma for inhibiting Aβ aggregation. PM assembly was achieved by charge-converting 3D6-Fab through pH-sensitive citraconylation to allow complexation with reductive-sensitive cationic...
CD30 is a member of the tumor necrosis factor receptor superfamily. Recently, blocking CD30-dependent intracellular signaling has emerged as potential strategy for immunological regulation. Development antibody-based antagonists therefore significant interest. However, key challenge that bivalent form natural antibody can crosslink molecules, leading to signal transduction even in absence specific ligand, CD153. Biparatopic antibodies (BpAbs) offer solution, using two different variable...
The gradual removal of the denaturing reagent guanidine HCl (GdnHCl) using stepwise dialysis with introduction an oxidizing and l-arginine resulted in highly efficient refolding various denatured single-chain Fv fragments (scFvs) from inclusion bodies expressed in<i>Escherichia coli.</i> In this study, influence additives on intermediates scFv was carefully analyzed basis dialysis, it revealed that additive effect critically changes pathway refolding. Circular dichroism tryptophan...