A5101465501- Plant Gene Expression Analysis
- Plant biochemistry and biosynthesis
- Heat Transfer and Boiling Studies
- Gut microbiota and health
- Phytochemicals and Antioxidant Activities
- Fusion materials and technologies
- Fluid Dynamics and Mixing
- Synthesis and Reactivity of Heterocycles
- Psychological Testing and Assessment
- Biochemical and biochemical processes
- Muon and positron interactions and applications
- Natural Language Processing Techniques
- Heat shock proteins research
- DNA and Nucleic Acid Chemistry
- Speech and dialogue systems
- Textile materials and evaluations
- Nuclear Materials and Properties
- Light effects on plants
- Plant tissue culture and regeneration
- Supramolecular Chemistry and Complexes
- Genomics and Chromatin Dynamics
- Probiotics and Fermented Foods
- Plant Molecular Biology Research
- Graphite, nuclear technology, radiation studies
- Synthesis and pharmacology of benzodiazepine derivatives
The University of Tokyo
1997-2025
Showa Women's University
2024
Suntory (Japan)
2006-2023
The Nakamura Hajime Eastern Institute
2007-2021
Asahi Kasei (Japan)
2016-2020
Juntendo University Urayasu Hospital
2020
Innovation Center of NanoMedicine
2020
Tokyo University of Foreign Studies
2013-2017
Kashiwa Municipal Hospital
2015
Toray Industries, Inc. (Japan)
2015
In addition to the five 70-kDa heat shock proteins (HSP70) common germ cells and somatic tissues of mammals, spermatogenic synthesize HSP70-2 during meiosis. To determine if this unique stress protein has a critical role in meiosis, we used gene-targeting techniques disrupt Hsp70-2 mice. Male mice homozygous for mutant allele (Hsp70-2 -/-) did not HSP70-2, lacked postmeiotic spermatids mature sperm, were infertile. However, neither meiosis nor fertility was affected female -/- We previously...
Flower color is mainly determined by anthocyanins. Rosa hybrida lacks violet to blue flower varieties due the absence of delphinidin-based anthocyanins, usually major constituents and flowers, because roses do not possess flavonoid 3',5'-hydoxylase (F3'5'H), a key enzyme for delphinidin biosynthesis. Other factors such as presence co-pigments vacuolar pH also affect color. We analyzed composition hundreds rose cultivars measured their petal juice in order select hosts genetic transformation...
This work presents the genome sequencing of lager brewing yeast (Saccharomyces pastorianus) Weihenstephan 34/70, a strain widely used in beer brewing. The 25 Mb comprises two nuclear sub-genomes originating from Saccharomyces cerevisiae and bayanus one circular mitochondrial S. bayanus. Thirty-six different types chromosomes were found including eight with translocations between sub-genomes, whose breakpoints are within orthologous open reading frames. Several gene loci responsible for...
We have studied Fermi surface properties of LaRhIn 5 and CeTIn (T: Co, Rh Ir) via the de Haas–van Alphen (dHvA) experiment. The a non-4 f reference compound is quasi-two dimensional, reflecting unique tetragonal crystal structure, which consistent with result FLAWP energy band calculations. an antiferromagnet CeRhIn similar to that , although cyclotron mass in roughly by one order larger than . Both surfaces are, however, different from those CeCoIn CeIrIn are well explained 4 -itinerant...
Flower color is most often conferred by colored flavonoid pigments. Aurone flavonoids confer a bright yellow on flowers such as snapdragon (Antirrhinum majus) and dahlia (Dahlia variabilis). A. majus aureusidin synthase (AmAS1) was identified the key enzyme that catalyzes aurone biosynthesis from chalcones, but transgenic overexpressing AmAS1 gene failed to produce aurones. Here, we report chalcone 4'-O-glucosyltransferase (4'CGT) essential for coloration in vivo. Coexpression of Am4'CGT...
Recent culture-independent studies have revealed that a healthy vaginal ecosystem harbors surprisingly complex assemblage of microorganisms. However, the spatial distribution and composition microbial populations not been investigated using molecular methods. Here, we evaluated site-specific within examined influence sampling technique in detection microbiota. 16S rRNA gene clone libraries were prepared from samples obtained different locations (cervix, fornix, outer canal) by methods...
Chrysanthemums (Chrysanthemum×morifolium Ramat.) are an important cut-flower and potted plant crop in the horticultural industry world wide. express flavonoid 3'-hydroxylase (F3'H) gene thus accumulate anthocyanins derived from cyanidin their inflorescences which appear pink/red. Delphinidin-based lacking due to deficiency of a 3', 5'-hydroxylase (F3'5'H), so violet/blue chrysanthemum flower colors not found. In this study, together with optimization transgene expression selection host...
Delivering therapeutic antibodies into the brain across blood–brain barrier at a level is promising while challenging approach in treatment of neurological disorders. Here, we present polymeric nanomicelle (PM) system capable delivering therapeutically effective levels 3D6 antibody fragments (3D6-Fab) parenchyma for inhibiting Aβ aggregation. PM assembly was achieved by charge-converting 3D6-Fab through pH-sensitive citraconylation to allow complexation with reductive-sensitive cationic...
The present study examined the occurrence of apoptotic cell death in testis wild-type mice from postnatal days 3 to 26 and juvenile Hsp70-2 knockout mice. Adult males are infertile lack spermatids spermatozoa (Dix et al. [1996a] Proc. Natl. Acad. Sci. U.S.A. 93:3264–3268). To identify types undergoing apoptosis, we also relationship between expression pattern gene product (heat-shock protein 70-2 [HSP70-2]; marker for spermatocytes spermatids), germ nuclear antigen 1 (GCNA1; spermatogonia...
Abstract Background : Programmed cell death is an essential event during mammalian morphogenesis which eliminates unnecessary cells to accomplish histogenesis and organogenesis. Cell in interdigital spaces of the developing limb a classical example morphogenetic death. We investigated whether programmed tissue mice apoptosis with fragmentation nuclear DNA also examined sequentially occurrence proliferation mouse fetuses analyze their interrelation. Methods apoptotic limbs by using Nile blue...
The draft genome sequence of a wild rose (Rosa multiflora Thunb.) was determined using Illumina MiSeq and HiSeq platforms. total length the scaffolds 739,637,845 bp, consisting 83,189 scaffolds, which close to 711 Mbp estimated by k-mer analysis. N50 90,830 extent longest 1,133,259 bp. average GC content 38.9%. After gene prediction, 67,380 candidates exhibiting homology known genes domains were extracted, included complete partial structures. This large number for diploid plant may reflect...
Sperm utilize glycolysis to generate ATP required for motility, and several spermatogenic cell-specific glycolytic isozymes are associated with the fibrous sheath (FS) in principal piece of sperm flagellum. We used proteomics molecular biology approaches confirm earlier reports that a novel enolase is present mouse sperm. then found pan-enolase antibody, but not antibodies ENO2 ENO3, recognized protein Database analyses identified two previously uncharacterized family-like candidate genes,...
Abstract In the flowers of important ornamental Compositae plants, anthocyanins generally carry malonyl group(s) at their 3-glucosyl moiety. this study, for first time to our knowledge, we have identified a cDNA coding 3-glucoside-specific malonyltransferase anthocyanins, i.e. malonyl-coenzyme A:anthocyanidin 3-O-glucoside-6"-O-malonyltransferase, from dahlia (Dahlia variabilis) flowers. We isolated full-length (Dv3MaT) on basis amino acid sequences specifically conserved among anthocyanin...
Post-transcriptional gene silencing, such as antisense suppression, sense suppression (or cosuppression), and RNAi, is often used to down-regulate a target in transgenic plants. Novel flower color industrially important; furthermore, convenient tool monitor the stability of silencing. Previously, we obtained white torenia through chalcone synthase or dihydroflavonol 4-reductase (Suzuki et al. 2000). However, their phenotypes were not stable. In this study, suppressed anthocyanidin using...
Petunia flower colors are mainly due to flavonoids. The color of commercial varieties hybrida was successfully modified by the suppression endogenous flavonoid biosynthetic genes, expression a hetelorogous gene, and combination both. Flower changed from purple almost white or red gene expression, orange down-regulation 3′-hydroxylase rose dihydroflavonol 4-reductase violet pale flavonol synthase flavone gene. These results clearly indicate usefulness metabolic engineering pathway modify...
Abstract Anthocyanins are a chemically diverse class of secondary metabolites that color most flowers and fruits. They consist three aromatic rings can be substituted with hydroxyl, sugar, acyl, methyl groups in variety patterns depending on the plant species. To understand how such chemical diversity evolved, we isolated characterized METHYLATION AT THREE2 (MT2) two FIVE (MF) loci from Petunia spp., which direct anthocyanin methylation petals. The proteins encoded by MT2 duplicated MF1 MF2...
Abstract Flow cytometry can provide detailed information about protein expression on cell surface and is, therefore, widely used in clinical testing. However, owing to the limited sensitivity of fluorescence signals, detection low‐expression markers is challenging. The present report describes a DNA‐mediated, on‐membrane assembly conjugated polymer nanoparticles (Pdots) that amplifies signal from for sensitive via flow cytometry. Single‐stranded DNA (ssDNA)‐conjugated antibodies are first...
Background A number of immunotherapeutic approaches have been developed and are entering the clinic. Bispecific antibodies (BsAbs) one these modalities induce robust efficacy by endogenous T cells in several hematological malignancies. However, most treated patients experience only a temporary benefit. Currently available BsAbs provide anti-CD3 antibody-mediated T-cell stimulation, but not costimulation or cytokine signaling essential for full activation. Here, we hypothesized that...