A5014203330- Plant Pathogens and Fungal Diseases
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Resistance
- Forest Insect Ecology and Management
- Mycorrhizal Fungi and Plant Interactions
- Bioenergy crop production and management
- Plant Disease Resistance and Genetics
- Forest Ecology and Biodiversity Studies
- Plant-Microbe Interactions and Immunity
- Plant and Fungal Interactions Research
- Genetic factors in colorectal cancer
- Cancer-related Molecular Pathways
- RNA Research and Splicing
- Biofuel production and bioconversion
- Genetic diversity and population structure
- RNA and protein synthesis mechanisms
- Plant Virus Research Studies
- Epigenetics and DNA Methylation
- Plant and animal studies
- Virus-based gene therapy research
- Cancer-related gene regulation
- Wheat and Barley Genetics and Pathology
- Insect symbiosis and bacterial influences
- Forest ecology and management
- Entomopathogenic Microorganisms in Pest Control
University of British Columbia
2016-2025
Université Laval
2016-2025
Okanagan University College
2010-2024
École Polytechnique Fédérale de Lausanne
2021
Canadian Food Inspection Agency
2016-2021
Oregon State University
2021
Natural Resources Canada
2010-2019
Canadian Forest Service
2009-2019
Qatar Airways (Qatar)
2018-2019
Weill Cornell Medical College in Qatar
2018-2019
Six DNA regions were evaluated as potential barcodes for Fungi , the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region mitochondrial cytochrome c oxidase subunit 1 used animal barcode was excluded marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from nuclear ribosomal RNA cistron compared together with three representative protein-coding genes (largest...
Hereditary nonpolyposis colorectal cancer (HNPCC), also known as Lynch syndrome, is a common autosomal dominant syndrome characterized by early age at onset, neoplastic lesions, and microsatellite instability (MSI). Because cancers with MSI account for approximately 15% of all because the need better understanding clinical histologic manifestations HNPCC, National Cancer Institute hosted an international workshop on HNPCC in 1996, which led to development Bethesda Guidelines identification...
Rust fungi are some of the most devastating pathogens crop plants. They obligate biotrophs, which extract nutrients only from living plant tissues and cannot grow apart their hosts. Their lifestyle has slowed dissection molecular mechanisms underlying host invasion avoidance or suppression innate immunity. We sequenced 101-Mb genome Melampsora larici - populina , causal agent poplar leaf rust, 89-Mb Puccinia graminis f. sp. tritici wheat barley stem rust. then compared 16,399 predicted...
The class Dothideomycetes is one of the largest groups fungi with a high level ecological diversity including many plant pathogens infecting broad range hosts. Here, we compare genome features 18 members this class, 6 necrotrophs, 9 (hemi)biotrophs and 3 saprotrophs, to analyze structure, evolution, diverse strategies pathogenesis. most likely evolved from common ancestor more than 280 million years ago. sequences differ dramatically in size due variation repetitive content, but show much...
DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate sequence data, tied to both correct taxonomic names and clearly annotated specimen has never been greater. Furthermore, growing number of molecular ecology microbiome projects using high-throughput sequencing require fast effective methods en masse assignments. In this article, we focus on selecting re-annotating a set marker reference...
We sequenced and compared the genomes of Dothideomycete fungal plant pathogens Cladosporium fulvum (Cfu) (syn. Passalora fulva) Dothistroma septosporum (Dse) that are closely related phylogenetically, but have different lifestyles hosts. Although both fungi grow extracellularly in close contact with host mesophyll cells, Cfu is a biotroph infecting tomato, while Dse hemibiotroph pine. The these similar set genes (70% gene content homologs), differ significantly size (Cfu >61.1-Mb; 31.2-Mb),...
Summary A novel method, Random Amplified Microsatellites (RAMS, due to the nature of amplified markers as two randomly microsatellites with intervening sequence), was applied generate DNA in a variety fungi ( Armillaria cepistipes, Gremmeniella abietina, Heterobasidion annosum, Pbytophthora cactorum, Phlebiopsis gigantea , and Stereum sanguinolentum ). It is based on polymerase chain reaction (PCR), uses primers containing microsatellite sequences degenerate anchors at 5' end. The method...
In western North America, the current outbreak of mountain pine beetle (MPB) and its microbial associates has destroyed wide areas lodgepole forest, including more than 16 million hectares in British Columbia. Grosmannia clavigera (Gc), a critical component outbreak, is symbiont MPB pathogen trees. To better understand interactions between Gc, MPB, hosts, we sequenced ∼30-Mb Gc genome assembled it into 18 supercontigs. We predict 8,314 protein-coding genes, support gene models with proteome,...
Sequencing-by-synthesis technologies can reduce the cost of generating de novo genome assemblies. We report a method for assembling draft sequences eukaryotic organisms that integrates sequence information from different sources, and demonstrate its effectiveness by an approximately 32.5 Mb forest pathogen Grosmannia clavigera, ascomycete fungus. also developed assessing assemblies using Illumina paired end read data how we are it to guide future finishing. Our results be accurately...
The obligate biotrophic rust fungus Melampsora larici-populina is the most devastating and widespread pathogen of poplars. Studies over recent years have identified various small secreted proteins (SSP) from plant filamentous pathogens highlighted their role as effectors in host-pathogen interactions. analysis M. genome sequence has revealed presence 1,184 SSP-encoding genes this fungus. In present study, expression evolutionary dynamics these SSP were investigated to pinpoint arsenal...
Abstract Stomata are essential for diffusive entry of gases to support photosynthesis, but may also expose internal leaf tissues pathogens. To uncover trade‐offs in range‐wide adaptation relating stomata, we investigated the underlying genetics stomatal traits and linked variability these with geoclimate, ecophysiology, condensed foliar tannins pathogen susceptibility black cottonwood ( P opulus trichocarpa ). Upper (adaxial) lower (abaxial) were measured from 454 accessions collected...
Black Sigatoka or black leaf streak disease, caused by the Dothideomycete fungus Pseudocercospora fijiensis (previously: Mycosphaerella fijiensis), is most significant foliar disease of banana worldwide. Due to lack effective host resistance, management this requires frequent fungicide applications, which greatly increase economic and environmental costs produce banana. Weekly applications in plantations lead rapid evolution fungicide-resistant strains within populations causing...
The sudden oak and larch death pathogen Phytophthora ramorum emerged simultaneously in the United States on Europe Rhododendron 1990s. This has had a devastating impact plantations Kingdom as well mixed conifer forests Western States. Since discovery of this pathogen, large body research provided novel insights into emergence, epidemiology, genetics pandemic. Genetic genomic resources developed for P. have been instrumental improving our understanding evolution, ecology disease. recent...
Tumor cell lines are commonly used as experimental tools in cancer research, but their relevance for the vivo situation is debated. In a series of 11 microsatellite stable (MSS) and 9 unstable (MSI) colon primary carcinomas (25 MSS 28 MSI) with known ploidy stem line APC, KRAS, TP53 mutation status, we analyzed promoter methylation following genes: hMLH1, MGMT, p16 INK4a (CDKN2A α-transcript), p14 ARF β-transcript), E-cadherin (CDH1). We compared DNA profiles those tumors. Finally, examined...
Abstract Our study evaluated in silico the potential of 14 mitochondrial genes encoding subunits respiratory chain complexes, including cytochrome c oxidase I ( CO1 ), as Basidiomycota DNA barcode. Fifteen complete and partial genomes were recovered characterized this study. Mitochondrial showed high values molecular divergence, indicating a for resolution lower‐level relationships. However, numerous introns occurred well six other genes, potentially interfering with polymerase reaction...
Damage or mortality from pathogens can reduce productivity of forest plantations, as well significantly harm natural ecosystems. Genetic resistance within the host species is first line defense for tree species. Resistance breeding programs native fusiform rust and exotic (to North America) white pine blister diseases are two longest concerted efforts in trees, spanning more than 50 years. Advances developing greater genetic have been made both pathosystems, but unique challenges...