Karen W. Hughes
- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Lichen and fungal ecology
- Fungal Biology and Applications
- Yeasts and Rust Fungi Studies
- Plant tissue culture and regeneration
- Plant Pathogens and Resistance
- Bryophyte Studies and Records
- Plant and Fungal Species Descriptions
- Marine and coastal plant biology
- Plant and animal studies
- Plant Diversity and Evolution
- Plant Genetic and Mutation Studies
- Genomics and Phylogenetic Studies
- Botanical Research and Applications
- Plant Parasitism and Resistance
- Botany and Plant Ecology Studies
- Plant Reproductive Biology
- Botanical Research and Chemistry
- Light effects on plants
- Forest Ecology and Biodiversity Studies
- Legume Nitrogen Fixing Symbiosis
- Plant and fungal interactions
- Silymarin and Mushroom Poisoning
- Plant-Microbe Interactions and Immunity
University of Tennessee at Knoxville
2015-2024
SUNY Upstate Medical University
2024
University of Liverpool
2022
Public Health Wales
2022
Bangor University
2022
Uppsala University
2018
Evolutionary Genomics (United States)
2014
Fera Science (United Kingdom)
2012
Six DNA regions were evaluated as potential barcodes for Fungi , the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region mitochondrial cytochrome c oxidase subunit 1 used animal barcode was excluded marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from nuclear ribosomal RNA cistron compared together with three representative protein-coding genes (largest...
P. Brandon Matheny*, Judd M. Curtisa, Valérie Hofstetterb, Catherine Aimec, Jean-Marc Moncalvod, Zai-Wei Ge, Zhu-Liang Yange, Jason C. Slotf, Joseph F. Ammiratig, Timothy J. Baronih, Neale L. Bougheri, Karen W. Hughesj, D. Jean Lodgek, Richard Kerriganl, Michelle T. Seidlm, Duur K. Aanenn, Matthew DeNitiso, Graciela Danielep, Dennis E. Desjardinq, Bradley R. Kroppr, Lorelei Norvells, Andrew Parkert, Else Vellingau, Rytas Vilgalysb & David S. Hibbettaa Biology Department, Clark University,...
DNA phylogenetic comparisons have shown that morphology-based species recognition often underestimates fungal diversity. Therefore, the need for accurate sequence data, tied to both correct taxonomic names and clearly annotated specimen has never been greater. Furthermore, growing number of molecular ecology microbiome projects using high-throughput sequencing require fast effective methods en masse assignments. In this article, we focus on selecting re-annotating a set marker reference...
An overview of the phylogeny Agaricales is presented based on a multilocus analysis six-gene region supermatrix. Bayesian analyses 5611 nucleotide characters rpb1, rpb1-intron 2, rpb2 and 18S, 25S, 5.8S ribosomal RNA genes recovered six major clades, which are recognized informally labeled Agaricoid, Tricholomatoid, Marasmioid, Pluteoid, Hygrophoroid Plicaturopsidoid clades. Each clade discussed in terms key morphological ecological traits. At least 11 origins ectomycorrhizal habit appear to...
Abstract Mushroom-forming fungi (Agaricomycetes) have the greatest morphological diversity and complexity of any group fungi. They radiated into most niches fulfil diverse roles in ecosystem, including wood decomposers, pathogens or mycorrhizal mutualists. Despite importance mushroom-forming fungi, large-scale patterns their evolutionary history are poorly known, part due to lack a comprehensive dated molecular phylogeny. Here, using multigene genome-based data, we assemble 5,284-species...
Keynote Address.- A Random Walk Through Plant Biology.- Regeneration Phenomena.- Patterns of Development in Culture.- Somatic Embryogenesis and Its Consequences the Gramineae.- Embryo Ontogeny Tissue Cultures Orchardgrass.- Developmental Mechanisms.- Factors Affecting Processes Alfalfa Cell Cultures.- Influencing Tomato Protoplast Development.- An Embryogenic Culture Soybean: Towards a General Theory Embryogenesis.- Applications Systems.- for Mass Cloning Crop Plants.- Bioreactor Technology...
Molecular phylogenies using 1–4 gene regions and information on ecology, morphology pigment chemistry were used in a partial revision of the agaric family Hygro- phoraceae. The phylogenetically supported genera we recognize here Hygrophoraceae based these previous analyses are: Acantholichen, Ampulloclitocybe, Arrhenia, Cantharellula, Cantharocybe, Chromosera, Chrysomphalina, Cora, Corella, Cuphophyllus, Cyphellostereum, Dictyonema, Eonema, Gliophorus, Haasiella, Humidicutis, Hygroaster,...
Lentinula is a broadly distributed group of fungi that contains the cultivated shiitake mushroom, L. edodes . We sequenced 24 genomes representing eight described species and several unnamed lineages from 15 countries on four continents. comprises major clades arose in Oligocene, three Americas one Asia–Australasia. To expand sampling mushrooms, we assembled 60 China were previously published as raw Illumina reads added them to our dataset. sensu lato (s. lat.) may warrant recognition...
Agaric fungi of the southern Appalachian Mountains including Great Smoky National Park are often heterozygous for rDNA internal transcribed spacer region (ITS) with >42% collections showing some heterozygosity indels and/or base-pair substitutions. For these collections, intra-individual haplotype divergence is typically less than 2%, but 3% exceeds that figure. We hypothesize high due to hybridization between agaric divergent haplotypes, possibly migrants from geographically isolated...
Following a late fall wildfire in 2016 the Great Smoky Mountains National Park, pyrophilous fungi burn zones were documented over 2-y period with respect to severity and phenology. Nuc rDNA internal transcribed spacer (ITS1-5.8S-ITS2 = ITS) barcodes obtained confirm morphological evaluations. Forty-one taxa of Ascomycota Basidiomycota identified from sites categorized as fruiting only response fire or enhanced by fire. Twenty-two species Pezizales (Ascomycota) among earliest form ascomata...
Previous studies based on morphology and ability to intercross suggested that new biological morphological species were sheltered within the genus Flammulina as well varieties F. velutipes. The currently described or proposed Northern Hemisphere are "mexicana, "F. populicola, rossica, ononidis, F elastica, fennae Within velutipes var. velutipes, "lupinicola," lactea. ribosomal ITS1-5.8S-ITS2 region was sequenced for eighteen geographically diverse collections, including three varieties....
Three species of the Funariaceae (Physcomitrella patens (Hedw.) B.S.G., Physcomitrium pyriforme Hampe and Funaria hygrometrica Hedw.) have been cultured under sterile conditions on Knop's inorganic me- dium (liquid agar) to determine minimum time nec- essary for completion sexual life cycle (spore spore). According data presented, F. may be completed in 4 months, P. 3 months 2 months. In all three critical factor induction maturation gametangia is cold temperature. Delineation makes these...