- Mycorrhizal Fungi and Plant Interactions
- Plant Pathogens and Fungal Diseases
- Fungal Biology and Applications
- Lichen and fungal ecology
- Yeasts and Rust Fungi Studies
- Botany and Plant Ecology Studies
- Plant and Fungal Species Descriptions
- Forest Ecology and Biodiversity Studies
- Bryophyte Studies and Records
- Mycotoxins in Agriculture and Food
- Plant Parasitism and Resistance
- Phytoplasmas and Hemiptera pathogens
- Plant Diversity and Evolution
- Environmental DNA in Biodiversity Studies
- Ecology and Conservation Studies
- Soil Carbon and Nitrogen Dynamics
- Gestational Trophoblastic Disease Studies
- Plant and fungal interactions
- Phytochemistry Medicinal Plant Applications
- Nematode management and characterization studies
- Genetic diversity and population structure
- Protist diversity and phylogeny
- Botanical Research and Applications
- Historical and Religious Studies of Rome
- Scientific Computing and Data Management
Staatliches Museum für Naturkunde Stuttgart
2015-2024
Meise Botanic Garden
2019-2021
Royal Holloway University of London
2021
National Central University
2018
Naturalis Biodiversity Center
2018
Uppsala University
2018
Ghent University
2015-2016
University of London
2015
Polish Academy of Sciences
2015
Museum für Naturkunde
2014
Six DNA regions were evaluated as potential barcodes for Fungi , the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region mitochondrial cytochrome c oxidase subunit 1 used animal barcode was excluded marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from nuclear ribosomal RNA cistron compared together with three representative protein-coding genes (largest...
Abstract The nuclear ribosomal internal transcribed spacer ( ITS ) region is the formal fungal barcode and in most cases marker of choice for exploration diversity environmental samples. Two problems are particularly acute pursuit satisfactory taxonomic assignment newly generated sequences: (i) lack an inclusive, reliable public reference data set (ii) means to refer species, which no Latin name available a standardized stable way. Here, we report on progress these regards through further...
• Identification of ectomycorrhizal (ECM) fungi is often achieved through comparisons ribosomal DNA internal transcribed spacer (ITS) sequences with accessioned deposited in public databases. A major problem encountered that annotation the these databases not always complete or trustworthy. In order to overcome this deficiency, we report on UNITE, an open-access database. UNITE comprises well annotated fungal ITS from defined herbarium specimens include full reference identification data,...
Species identification lies at the heart of biodiversity studies that has in recent years favoured DNA-based approaches. Microbial Biological Resource Centres are a rich source for diverse and high-quality reference materials microbiology, yet strains preserved these biobanks have been exploited only on limited scale to generate DNA barcodes. As part project funded Netherlands barcode specimens major national biobanks, sequences two nuclear ribosomal genetic markers, Internal Transcribed...
The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional ITS rDNA primary barcode. Amplification efficiencies 14 (partially) targeting eight genetic markers were tested across > 1 500 species (1 931 strains or specimens) outcomes almost twenty thousand (19 577) polymerase chain reactions evaluated. We several well-known that amplify: i) sections nuclear ribosomal RNA large...
The Amsterdam Declaration on Fungal Nomenclature was agreed at an international symposium convened in 19-20 April 2011 under the auspices of International Commission Taxonomy Fungi (ICTF). purpose to address issue whether or how current system naming pleomorphic fungi should be maintained changed now that molecular data are routinely available. is urgent as mycologists currently follow different practices, and no consensus achieved by a Special Committee appointed 2005 Botanical Congress...
DNA barcoding is a global initiative for species identification through sequencing of short sequence markers. Sequences two loci, ITS and LSU, were generated as barcode data all (ca. 9k) yeast strains included in the CBS collection, originally assigned to ca. 2 000 species. Taxonomic validation turned out be most severe bottleneck due large volume trace files lack reference sequences. We have analysed validated sequences automatically. Our analysis shows that there 6 9.5 % could not...
• Soil abiotic factors are considered to be important in determining the distribution of ectomycorrhizal (ECM) fungal species; however, there few field data support this. Here, we relate ECM species distributions changes soil chemistry along a short (90-m), natural nutrient gradient. The community was characterized, using morphological and molecular techniques, samples collected at 10-m intervals. There were pronounced structure transect, with many taxa showing discrete distributions....
Infrageneric relations of the genetically diverse milkcap genus Lactifluus (Russulales, Basidiomycota) are poorly known. Currently used classification systems still largely reflect traditional, mainly morphological, characters for infrageneric delimitations milkcaps. Increased sampling, combined with small-scale molecular studies, show that this is underexplored and in need revision. For study, we assembled an extensive dataset Lactifluus, comprising 80 % all known species 30 type...
Ectomycorrhizal (ECM) fungi are often considered to be most prevalent under conditions where organic sources of N predominate. However, ECM increasingly exposed nitrate from anthropogenic sources. Currently, the ability metabolize this is poorly understood. Here, growth was examined among 106 isolates, representing 68 species, on as sole source. In addition, occurrence genes coding for reductase enzyme (nar gene) in a broad range ectomycorrhizal investigated. All isolates grew nitrate, but...
This is the first study exclusively dedicated to of Hebeloma in Greenland. It based on almost 400 collections, great majority which were collected by three co-authors over a period 40 years and lodged fungarium Natural History Museum Copenhagen. The material was identified using molecular morphological methods. In total, 28 species recognized, 27 belonging sections, H. sects , Denudata Velutipes . One sampled new science here described as arcticum For all species, description, distribution...
Stipitate stereoid fungi are Basidiomycetes with a stipe, spathulate-to funnel-shaped pileus, smooth hymenophore, and hyaline, spores. Representatives of the genera Cotylidia, Cymatoderma, Muscinupta, Podoscypha Stereopsis were subjected to molecular phylogenetic analyses based on nuclear ribosomal large subunit, 5.8S ITS sequences. For four type species was included in analyses. radicans, Stereopsis, forms lineage corticioid Clavulicium globosum but could not be placed any presently...
The nuclear ribosomal internal transcribed spacer (nrITS) region has been widely used in fungal diversity studies. Environmental metabarcoding increased the importance of DNA barcode documenting and distribution. gap is seen as difference between intra- inter-specific pairwise distances a barcode. current understanding macrofungi limited, inhibiting development best practices applying nrITS toward research on diversity. This study examined using 5146 sequences representing 717 species from...
Six new sequestrate Lactarius species are described from tropical forests in South East Asia. Extensive macro- and microscopical descriptions illustrations of the main anatomical features provided. Similarities with other Russulales their phylogenetic relationships discussed. The placement within its subgenera is confirmed by a molecular phylogeny based on ITS, LSU rpb2 markers. A key taxa, including five known angiocarpous Asia reported to exude milk, given. diversity fungi areas considered...
Lactarius bisporus sp. nov. is described from primary tropical forest in Thailand. Morphological characters and DNA sequence data are given. Comparisons with the closely related angiocarpous taxon pomiolens provided.
Xerampelinae is a subsection composed of species ectomycorrhizal fungi belonging to the hyperdiverse and cosmopolitan genus Russula (Russulales). Species are recognized by their fishy or shrimp odor, browning context, green reaction iron sulfate. However, delimitation has traditionally relied on morphology analysis limited molecular data. Prior taxonomic work in led description as many 59 taxa Europe 19 North America. Here we provide first multilocus phylogeny European American members based...
Abstract On the basis of detailed morphological and molecular investigation, eighteen new species Inocybe ( I. alberichiana , beatifica bellidiana clandestina drenthensis dryadiana gaiana ghibliana grusiana knautiana lampetiana oetziana orionis plurabellae rivierana scolopacis sitibunda tiburtina ) are described. All them smooth-spored, most pruinose only in apical part stipe. The compared to 40 type specimens, all which described here for several (partial) ITS sequences have been generated....