A5038529807- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- Plant Pathogens and Fungal Diseases
- Methane Hydrates and Related Phenomena
- Gut microbiota and health
- Mycorrhizal Fungi and Plant Interactions
- Biofuel production and bioconversion
- Plant and fungal interactions
- Protist diversity and phylogeny
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Production and Characterization
- Ruminant Nutrition and Digestive Physiology
- Fungal Biology and Applications
- Environmental DNA in Biodiversity Studies
- Hydrocarbon exploration and reservoir analysis
- Metal Extraction and Bioleaching
- Marine and coastal ecosystems
- Vector-borne infectious diseases
- Atmospheric and Environmental Gas Dynamics
- Microbial bioremediation and biosurfactants
- Anaerobic Digestion and Biogas Production
- Molecular Biology Techniques and Applications
- Wheat and Barley Genetics and Pathology
- Yersinia bacterium, plague, ectoparasites research
- Insect symbiosis and bacterial influences
Oklahoma State University
2016-2025
Oklahoma State University Oklahoma City
2017-2025
University of Toronto
2023
Jackson State University
2023
New York University Press
2022
Cambridge University Press
2022
Oak Ridge National Laboratory
2021
Weatherford College
2021
Laboratory of Molecular Genetics
2018
University of Oklahoma
2001-2011
Six DNA regions were evaluated as potential barcodes for Fungi , the second largest kingdom of eukaryotic life, by a multinational, multilaboratory consortium. The region mitochondrial cytochrome c oxidase subunit 1 used animal barcode was excluded marker, because it is difficult to amplify in fungi, often includes large introns, and can be insufficiently variable. Three subunits from nuclear ribosomal RNA cistron compared together with three representative protein-coding genes (largest...
ABSTRACT Pyrosequencing-based 16S rRNA gene surveys are increasingly utilized to study highly diverse bacterial communities, with special emphasis on utilizing the large number of sequences obtained (tens hundreds thousands) for species richness estimation. However, it is not yet clear how operational taxonomic units (OTUs) and, hence, estimates determined using shorter fragments at different cutoffs correlates OTUs assigned longer, nearly complete fragments. We constructed a clone library...
ABSTRACT Anaerobic gut fungi represent a distinct early-branching fungal phylum (Neocallimastigomycota) and reside in the rumen, hindgut, feces of ruminant nonruminant herbivores. The genome an anaerobic isolate, Orpinomyces sp. strain C1A, was sequenced using combination Illumina PacBio single-molecule real-time (SMRT) technologies. large (100.95 Mb, 16,347 genes) displayed extremely low G+C content (17.0%), noncoding intergenic regions (73.1%), proliferation microsatellite repeats (4.9%),...
ABSTRACT Soil bacterial communities typically exhibit a distribution pattern in which most species are present low abundance. Due to the relatively small size of culture-independent sequencing surveys, detailed phylogenetic analysis rare members community is lacking. To gain access rarely sampled soil biosphere, we analyzed data set 13,001 near-full-length 16S rRNA gene clones derived from an undisturbed tall grass prairie central Oklahoma. Rare (empirically defined at two different...
ABSTRACT An artesian sulfide- and sulfur-rich spring in southwestern Oklahoma is shown to sustain an extremely rich diverse microbial community. Laboratory incubations autoradiography studies indicated that active sulfur cycling occurring the abundant mats at Zodletone spring. Anoxygenic phototrophic bacteria oxidize sulfide sulfate, which reduced by sulfate-reducing bacterial populations. The community was analyzed cloning sequencing 16S rRNA genes. A large fraction (83%) of mat clones...
Hydraulic fracturing is used to increase the permeability of shale gas formations and involves pumping large volumes fluids into these formations. A portion frac fluid remains in formation after process complete, which could potentially contribute deleterious microbially induced processes natural wells. Here, we report on geochemical microbiological properties flowback waters from two newly drilled wells Barnett Shale North Central Texas. Most probable number studies showed that biocide...
We investigated the global distribution patterns and pangenomic diversity of candidate phylum "Latescibacteria" (WS3) in 16S rRNA gene as well metagenomic data sets. document distinct for various orders sets, with prevalence sediment_1 terrestrial, PBSIII_9 groundwater temperate freshwater, GN03 pelagic marine, saline-hypersaline, wastewater habitats. Using a fragment recruitment approach, we identified 68.9 Mb "Latescibacteria"-affiliated contigs publicly available sets comprising 73,079...
Summary We report on the genomic characterization of three novel classes in phylum Desulfobacterota. One class (proposed name Candidatus ‘Anaeroferrophillalia’) was characterized by heterotrophic growth capacity, either fermentatively or utilizing polysulfide, tetrathionate thiosulfate as electron acceptors. In absence organic carbon sources, autotrophic via Wood–Ljungdahl (WL) pathway and using hydrogen Fe(II) an donor is also inferred for members ‘Anaeroferrophillalia’. The second...
Recent discoveries suggest that the candidate superphyla Patescibacteria and DPANN constitute a large fraction of phylogenetic diversity Bacteria Archaea. Their small genomes limited coding potential have been hypothesized to be ancestral adaptations obligate symbiotic lifestyles. To test this hypothesis, we performed cell-cell association, genomic, analyses on 4,829 individual cells Archaea from 46 globally distributed surface subsurface field samples. This confirmed ubiquity abundance in...
Survival and growth of the anaerobic gut fungi (AGF; Neocallimastigomycota) in herbivorous necessitate possession multiple abilities absent other fungal lineages. We hypothesized that horizontal gene transfer (HGT) was instrumental forging evolution AGF into a phylogenetically distinct gut-dwelling lineage. The patterns HGT were evaluated transcriptomes 27 strains, 22 which isolated sequenced this study, 4 genomes broadly covering breadth diversity. identified 277 incidents transcriptomes,...
Abstract Fungi are one of the most diverse groups organisms with an estimated number species in range 2–3 million. The higher-level ranking fungi has been discussed framework molecular phylogenetics since Hibbett et al., and definition higher ranks (e.g., phyla) ‘true fungi’ have revised several subsequent publications. Rapid accumulation novel genomic data advancements now facilitate a robust precise foundation for classification within kingdom. This study provides updated kingdom , drawing...
Accurate assessment of the fate hydrocarbons spilt in aquifers is essential for gauging associated health and ecological risks. Regulatory pressure to actively remediate such contaminated ecosystems can be substantially diminished if solid evidence situ microbial destruction pollutants obtained. In laboratory incubations, sediment-associated microorganisms from a gas condensate-contaminated aquifer anaerobically biodegraded toluene, ethylbenzene, xylene, toluic acid isomers with...
ABSTRACT We investigated the phylogenetic diversity and metabolic capabilities of members phylum Planctomycetes in anaerobic, sulfide-saturated sediments a mesophilic spring (Zodletone Spring) southwestern Oklahoma. Culture-independent analyses 16S rRNA gene sequences generated using -biased primer pairs suggested that an extremely diverse community is present at spring. Although are phylogenetically affiliated with cultured heterotrophic were identified, majority belonged to several...
Biofuel research is currently an area of immense interest due to the increase in global energy demand by emerging economies and recent increases oil prices. Multiple approaches are being researched for use microorganisms production various biofuel (e.g. alcohols, hydrogen, biodiesel, biogas) from multiple starting materials. This review provides a brief overview on underway laboratory industrial scales biofuels, with specific emphasis economic viability utilized.