A5015085792- Microbial Community Ecology and Physiology
- Genomics and Phylogenetic Studies
- SARS-CoV-2 and COVID-19 Research
- Protist diversity and phylogeny
- RNA modifications and cancer
- Meat and Animal Product Quality
- Epigenetics and DNA Methylation
- Gut microbiota and health
- Bacteriophages and microbial interactions
- Antimicrobial Resistance in Staphylococcus
- CRISPR and Genetic Engineering
- Invertebrate Immune Response Mechanisms
- Legume Nitrogen Fixing Symbiosis
- Clostridium difficile and Clostridium perfringens research
- Insect Resistance and Genetics
- Streptococcal Infections and Treatments
- Bacterial Genetics and Biotechnology
- Microbial Natural Products and Biosynthesis
- Food Allergy and Anaphylaxis Research
- vaccines and immunoinformatics approaches
- Neurobiology and Insect Physiology Research
- Animal Nutrition and Physiology
- Sensory Analysis and Statistical Methods
- Wastewater Treatment and Nitrogen Removal
- Environmental DNA in Biodiversity Studies
Oklahoma State University
2022-2025
Oklahoma State University Oklahoma City
2022-2023
University of Oklahoma
2011-2022
Kansas State University
2017-2019
Arkansas Agricultural Experiment Station
2017
University of Oklahoma Health Sciences Center
2001-2002
Legumes (Fabaceae or Leguminosae) are unique among cultivated plants for their ability to carry out endosymbiotic nitrogen fixation with rhizobial bacteria, a process that takes place in specialized structure known as the nodule. belong one of two main groups eurosids, Fabidae, which includes most species capable fixation. comprise several evolutionary lineages derived from common ancestor 60 million years ago (Myr ago). Papilionoids largest clade, dating nearly origin legumes and containing...
Streptococcus mutans is the leading cause of dental caries (tooth decay) worldwide and considered to be most cariogenic all oral streptococci. The genome S. UA159, a serotype c strain, has been completely sequenced composed 2,030,936 base pairs. It contains 1,963 ORFs, 63% which have assigned putative functions. analysis provides further insight into how adapted surviving environment through resource acquisition, defense against host factors, use gene products that maintain its niche...
The 1,852,442-bp sequence of an M1 strain Streptococcus pyogenes , a Gram-positive pathogen, has been determined and contains 1,752 predicted protein-encoding genes. Approximately one-third these genes have no identifiable function, with the remainder falling into previously characterized categories known microbial function. Consistent observation that S. is responsible for wider variety human disease than any other bacterial species, more 40 putative virulence-associated identified....
ABSTRACT High-grain adaptation programs are widely used with feedlot cattle to balance enhanced growth performance against the risk of acidosis. This a high-grain diet from high-forage is known change rumen microbial population structure and help establish stable within rumen. Therefore, evaluate bacterial dynamics during diet, 4 ruminally cannulated beef steers were adapted using step-up regimen containing grain hay at ratios 20:80, 40:60, 60:40, 80:20. The populations evaluated each stage...
ABSTRACT Pyrosequencing-based 16S rRNA gene surveys are increasingly utilized to study highly diverse bacterial communities, with special emphasis on utilizing the large number of sequences obtained (tens hundreds thousands) for species richness estimation. However, it is not yet clear how operational taxonomic units (OTUs) and, hence, estimates determined using shorter fragments at different cutoffs correlates OTUs assigned longer, nearly complete fragments. We constructed a clone library...
ABSTRACT Anaerobic gut fungi represent a distinct early-branching fungal phylum (Neocallimastigomycota) and reside in the rumen, hindgut, feces of ruminant nonruminant herbivores. The genome an anaerobic isolate, Orpinomyces sp. strain C1A, was sequenced using combination Illumina PacBio single-molecule real-time (SMRT) technologies. large (100.95 Mb, 16,347 genes) displayed extremely low G+C content (17.0%), noncoding intergenic regions (73.1%), proliferation microsatellite repeats (4.9%),...
ABSTRACT Soil bacterial communities typically exhibit a distribution pattern in which most species are present low abundance. Due to the relatively small size of culture-independent sequencing surveys, detailed phylogenetic analysis rare members community is lacking. To gain access rarely sampled soil biosphere, we analyzed data set 13,001 near-full-length 16S rRNA gene clones derived from an undisturbed tall grass prairie central Oklahoma. Rare (empirically defined at two different...
In an effort to better understand the ancestral state of human distal gut microbiome, we examine feces retrieved from archaeological contexts (coprolites). To accomplish this, pyrosequenced 16S rDNA V3 region duplicate coprolite samples recovered three sites, each representing a different depositional environment: Hinds Cave (∼8000 years B.P.) in southern United States, Caserones (1600 northern Chile, and Rio Zape Mexico (1400 B.P.). Clustering algorithms grouped same site. Phyletic...
ABSTRACT An artesian sulfide- and sulfur-rich spring in southwestern Oklahoma is shown to sustain an extremely rich diverse microbial community. Laboratory incubations autoradiography studies indicated that active sulfur cycling occurring the abundant mats at Zodletone spring. Anoxygenic phototrophic bacteria oxidize sulfide sulfate, which reduced by sulfate-reducing bacterial populations. The community was analyzed cloning sequencing 16S rRNA genes. A large fraction (83%) of mat clones...
Sugarcane (Saccharum spp.) has become an increasingly important crop for its leading role in biofuel production. The high sugar content species S. officinarum is octoploid without known diploid or tetraploid progenitors. Commercial sugarcane cultivars are hybrids between and wild spontaneum with ploidy at approximately 12x. complex autopolyploid genome not been characterized the DNA sequence level.The microsynteny sorghum was assessed by comparing 454 pyrosequences of 20 bacterial artificial...
The 1,815,783-bp genome of a serotype M49 strain Streptococcus pyogenes (group A streptococcus [GAS]), NZ131, has been determined. This GAS (FCT type 3; emm pattern E), originally isolated from case acute post-streptococcal glomerulonephritis, is unusually competent for electrotransformation and used extensively as model organism both basic genetic pathogenesis investigations. As with the previously sequenced S. genomes, three unique prophages are major source diversity. Two clustered...
ABSTRACT We investigated the phylogenetic diversity and metabolic capabilities of members phylum Planctomycetes in anaerobic, sulfide-saturated sediments a mesophilic spring (Zodletone Spring) southwestern Oklahoma. Culture-independent analyses 16S rRNA gene sequences generated using -biased primer pairs suggested that an extremely diverse community is present at spring. Although are phylogenetically affiliated with cultured heterotrophic were identified, majority belonged to several...
Abstract Background Biological nitrogen fixation is a prokaryotic process that plays an essential role in the global cycle. Azorhizobium caulinodans ORS571 has dual capacity to fix both as free-living organism and symbiotic interaction with Sesbania rostrata . The host fast-growing, submergence-tolerant tropical legume on which A. can efficiently induce nodule formation root system adventitious rootlets located stem. Results 5.37-Mb genome consists of single circular chromosome overall...
The Human Microbiome Project (HMP) is one of the U.S. National Institutes Health Roadmap for Medical Research. Primary interests HMP include distinctiveness different gut microbiomes, factors influencing microbiome diversity, and functional redundancies members human microbiotas. In this present work, we contribute to these by characterizing two extinct microbiotas.We examine paleofecal samples originating from cave deposits in Durango Mexico dating approximately 1300 years ago....
Significance Current models for biological invasions are predominantly based on macroorganisms. Few invasion model assumptions have been validated microbial systems. Further research dynamics is required to determine whether macrobial appropriate microbes, as well understand present and future distributions of invasive microorganisms, particularly in the face contemporary environmental changes. We studied establishment an protist natural assemblages replicate experimental microcosms found...
ABSTRACT The archaeal community in a sulfide- and sulfur-rich spring with stream water salinity of 0.7 to 1.0% southwestern Oklahoma was studied by cloning sequencing 16S rRNA genes. Two clone libraries were constructed from sediments obtained at the hydrocarbon-exposed source microbial mats underlying flowing source. Analysis 113 clones library 65 mat revealed that majority belonged kingdom Euryarchaeota , while Crenarchaeota represented less than 10% clones. Euryarchaeotal orders...
H8 is derived from a collection of Salmonella enterica serotype Enteritidis bacteriophage. Its morphology and genomic structure closely resemble those bacteriophage T5 in the family Siphoviridae. infected S. serotypes Typhimurium Escherichia coli by initial adsorption to outer membrane protein FepA. Ferric enterobactin inhibited binding E. FepA (50% inhibition concentration, 98 nM), other ferric catecholate receptors (Fiu, Cir, IroN) did not participate phage adsorption. infection was TonB...
ABSTRACT Crc (catabolite repression control) protein of Pseudomonas aeruginosa has shown to be involved in carbon regulation several pathways. In this study, the role catabolite control been studied putida . The bkd operons P. and encode inducible multienzyme complex branched-chain keto acid dehydrogenase, which is regulated both species by repression. We report here that effect mediated Crc. A 13-kb cloned DNA fragment containing crc gene region was sequenced. regulates expression...
ABSTRACT We previously isolated a spontaneous mutant of Escherichia coli K-12, strain MG1655, following passage through the streptomycin-treated mouse intestine, that has colonization traits superior to wild-type parent (M. P. Leatham et al., Infect. Immun. 73: 8039–8049, 2005). This intestine-adapted ( E. MG1655*) grew faster on several different carbon sources than wild type and was nonmotile due deletion flhD gene. now report results high-throughput genomic analysis approaches further...
Summary High‐throughput pyrosequencing of SSU rDNA genes was used to obtain monthly snapshots eukaryotic and bacterial diversity community structure at two locations in L ake T exoma, a low salinity lake the south central U nited S tates, over 1 year. The experienced disturbance events (i) localized bloom P rymnesium parvum restricted one that lasted from J anuary A pril, (ii) large (17 cm), global rain event beginning M ay, overlaid onto seasonal environmental change. Eukaryotic species...
Abstract Blood serum is arguably the most analyzed biofluid for disease prediction and diagnosis. Herein, we benchmarked five different abundant protein depletion (SAPD) kits with regard to identification of disease‐specific biomarkers in human using bottom‐up proteomics. As expected, IgG removal efficiency among SAPD highly variable, ranging from 70% 93%. A pairwise comparison database search results showed a 10%–19% variation kits. Immunocapturing‐based against albumin outperformed others...
ABSTRACT The microeukaryotic community in Zodletone Spring, a predominantly anaerobic sulfide and sulfur-rich spring, was examined using an 18S rRNA gene cloning sequencing approach. majority of the 288 clones sequenced from three different locations at Spring belonged to Stramenopiles , Alveolata Fungi with members phylum Cercozoa order Diplomonadida family Jakobidae representing minor fraction clone library. No sequences suggesting presence novel kingdom level diversity were detected any...
ABSTRACT Immobilization of uranium in groundwater can be achieved through microbial reduction U(VI) to U(IV) upon electron donor addition. Microbial community structure was analyzed ethanol-biostimulated and control sediments from a high-nitrate (>130 mM), low-pH, uranium-contaminated site Oak Ridge, TN. Analysis small subunit (SSU) rRNA gene clone libraries polar lipid fatty acids revealed that biostimulation resulted general decrease bacterial diversity. Specifically, an increase the...