A5039002556- Plant-Microbe Interactions and Immunity
- Legume Nitrogen Fixing Symbiosis
- Mycorrhizal Fungi and Plant Interactions
- Plant nutrient uptake and metabolism
- Plant Molecular Biology Research
- Protist diversity and phylogeny
- Plant Parasitism and Resistance
- Plant Reproductive Biology
- Plant Pathogens and Fungal Diseases
- Plant Pathogenic Bacteria Studies
- Plant tissue culture and regeneration
- Infectious Disease Case Reports and Treatments
- Infections and bacterial resistance
- Enzyme Structure and Function
- Plant and animal studies
- Yeasts and Rust Fungi Studies
- Berry genetics and cultivation research
- Natural product bioactivities and synthesis
- Potato Plant Research
- Tuberculosis Research and Epidemiology
- Biochemical and Molecular Research
- Polysaccharides and Plant Cell Walls
- Photosynthetic Processes and Mechanisms
- Plant Disease Resistance and Genetics
- Palliative Care and End-of-Life Issues
University College West Flanders
2023-2025
Ghent University
2010-2022
New Mexico State University
2020
Ghent University Global Campus
2019
University of Chicago
2018
University College Ghent
2010-2013
Weatherford College
2012
University of Bologna
2011
GTx (United States)
2010
VIB-UGent Center for Plant Systems Biology
2007-2010
Abstract The importance of biostimulants, defined as plant growth-promoting agents that differ notably from fertilizers, is increasing steadily because their potential contribution to a worldwide strategy for securing food production without burdening the environment. Based on folkloric evidence and ethnographic studies, seaweeds have been useful diverse human activities through time, including medicine agriculture. Currently, seaweed extracts, especially those derived common brown alga...
The major cell wall polysaccharide of mycobacteria is a branched-chain arabinogalactan in which arabinan chains are attached to the 5 carbon some 6-linked galactofuranose residues; these composed exclusively D-arabinofuranose (Araf) residues. immediate precursor polymerized Araf decaprenylphosphoryl-D-Araf, derived from 5-phosphoribose 1-diphosphate (pRpp) an undefined manner. On basis time course, feedback, and chemical reduction experiment results we propose that decaprenylphosphoryl-Araf...
Decades ago, the importance of cytokinins (CKs) during Rhodococcus fascians pathology had been acknowledged, and an isopentenyltransferase gene characterized in fas operon linear virulence plasmid, but hitherto, no specific CK(s) could be associated with virulence. We show that CK receptors AHK3 AHK4 Arabidopsis thaliana are essential for symptom development, perception machinery is induced upon infection, underlining its central role symptomatology. Three classical CKs [isopentenyladenine,...
Abstract Background Biological nitrogen fixation is a prokaryotic process that plays an essential role in the global cycle. Azorhizobium caulinodans ORS571 has dual capacity to fix both as free-living organism and symbiotic interaction with Sesbania rostrata . The host fast-growing, submergence-tolerant tropical legume on which A. can efficiently induce nodule formation root system adventitious rootlets located stem. Results 5.37-Mb genome consists of single circular chromosome overall...
The role and metabolism of indole-3-acetic acid in gram-negative bacteria is well documented, but little known about biosynthesis regulation gram-positive bacteria. phytopathogen Rhodococcus fascians, a organism, incites diverse developmental alterations, such as leafy galls, on wide range plants. Phenotypic analysis gall suggests that auxin may play an important the development symptoms. We show here for first time R. fascians produces secretes acid. Interestingly, whereas...
The phytopathogenic actinomycete Rhodococcus fascians D188 relies mainly on the linear plasmid-encoded fas operon for its virulence. bacteria secrete six cytokinin bases that synergistically redirect developmental program of plant to stimulate proliferation young shoot tissue, thus establishing a leafy gall as niche. A yeast-based bioassay combined with profiling bacterial mutants revealed is essential enhanced production isopentenyladenine, trans-zeatin, cis-zeatin, and 2-methylthio...
Three virulence loci (fas, att, and hyp) of Rhodococcus fascians D188 have been identified on a 200-kb conjugative linear plasmid (pFiD188). The fas locus was delimited to 6.5-kb DNA fragment by insertion mutagenesis, single homologous disruptive recombination, in trans complementation different avirulent mutants. is arranged as large operon containing six open reading frames whose expression specifically induced during the interaction with host plants. One predicted protein P-450...
Abstract Rhodococcus fascians is a Gram-positive phytopathogen that induces shooty hyperplasia on its hosts through the secretion of cytokinins. Global transcriptomics using microarrays combined with profiling primary metabolites infected Arabidopsis (Arabidopsis thaliana) plants revealed this actinomycete modulated pathways to convert host into niche. The transcript data demonstrated R. leaves very characteristic mark pronounced cytokinin response illustrated by activation perception,...
Significance The regeneration of entire plants from explants is an important step in plant production and transformation protocols. Despite recent advances the knowledge on molecular basis regeneration, many aspects process causes recalcitrance are still poorly understood. We combined linkage with association mapping to find genes underlying natural variation shoot Arabidopsis . With this approach, we identified confirmed involvement RECEPTOR-LIKE PROTEIN KINASE1 as a previously unknown...
Leafy gall syndrome is the consequence of modified plant development in response to a mixture cytokinins secreted by biotrophic actinomycete Rhodococcus fascians. The similarity induced symptoms with phenotype mutants defective strigolactone biosynthesis and signalling prompted an evaluation involvement strigolactones this pathology. All tested strigolactone-related Arabidopsis thaliana were hypersensitive R. Moreover, treatment synthetic GR24 carotenoid cleavage dioxygenase inhibitor D2...
Decaprenylphosphoryl-d-arabinose, the lipid donor of mycobacterial d-arabinofuranosyl residues, is synthesized from phosphoribose diphosphate rather than a sugar nucleotide. The first committed step in process transfer 5-phosphoribosyl residue to decaprenyl phosphate form decaprenylphosphoryl-5-phosphoribose via 5-phospho-alpha-d-ribose-1-diphosphate:decaprenyl-phosphate 5-phospho-ribosyltransferase. A candidate for gene encoding this enzyme (Rv3806c) was identified Mycobacterium...
ABSTRACT The gram-positive plant pathogen Rhodococcus fascians provokes leafy gall formation on a wide range of plants through secretion signal molecules that interfere with the hormone balance host. Crucial virulence genes are located linear plasmid, and their expression is tightly controlled. A mutant mutation in chromosomal locus affected was isolated. gene vicA , which encodes malate synthase functional glyoxylate shunt Krebs cycle. VicA required for efficient planta growth symptomatic,...
Establishment of a successful symbiosis between rhizobia and legumes results from an elaborate molecular dialogue both partners. Bacterial nodulation (Nod) factors are indispensable for initiating plant responses, whereas bacterial surface polysaccharides important infection progression nodule development. The mutant ORS571-oac2 Azorhizobium caulinodans , affected in its polysaccharides, provokes defective interaction with host Sesbania rostrata . induced structures retarded development...
Abstract The biotrophic actinomycete Rhodococcus fascians has a profound impact on plant development and common aspect of the symptomatology is deformation infected leaves. In Arabidopsis (Arabidopsis thaliana), serrated leaf margins formed upon infection resemble phenotype transgenic plants with ectopic expression KNOTTED-like homeobox (KNOX) genes. Through transcript profiling, we demonstrate that class-I KNOX genes are transcribed in symptomatic Functional analysis revealed...
Rhodococcus fascians is currently the only phytopathogen of which virulence genes occur on a linear plasmid. To get insight into origin this replicon and strategy broad-spectrum phytopathogen, sequence plasmid strain D188, pFiD188, was determined. Analysis 198,917 bp revealed four syntenic regions with plasmids R. erythropolis, jostii, opacus, suggesting common these replicons. Mutational analysis pFi_086 pFi_102, similar to cutinases type IV peptidases, respectively, showed that conserved...
The natural products actinonin and matlystatin feature an N -hydroxy-2-pentyl-succinamyl (HPS) chemophore that facilitates metal chelation confers their metalloproteinase inhibitory activity. Actinonin is the most potent inhibitor of peptide deformylase (PDF) exerts antimicrobial herbicidal bioactivity by disrupting protein synthesis. Here, we used a genomics-led approach to identify candidate biosynthetic gene clusters (BGCs) hypothesized produce HPS-containing products. We show one these...
The ability of Rhodococcus fascians strain D188 to provoke leafy gall formation on a variety plant species is correlated with the linear plasmid pFiD188, which different pathogenicity loci were identified. att locus affects severity symptom development tobacco, whereas fas essential for virulence. To gain insight into function locus, sequence and expression analyses performed. contains nine open reading frames homologous arginine β‐lactam biosynthetic genes. gene transcriptionally induced by...