A5042069315- Fermentation and Sensory Analysis
- Yeasts and Rust Fungi Studies
- Antifungal resistance and susceptibility
- Spectroscopy Techniques in Biomedical and Chemical Research
- Spectroscopy and Chemometric Analyses
- Plant Pathogens and Fungal Diseases
- Microbial Metabolic Engineering and Bioproduction
- Horticultural and Viticultural Research
- Biofuel production and bioconversion
- Mycorrhizal Fungi and Plant Interactions
- Bacterial Identification and Susceptibility Testing
- Antimicrobial agents and applications
- Advanced Chemical Sensor Technologies
- Fungal Infections and Studies
- Bacterial biofilms and quorum sensing
- Metabolomics and Mass Spectrometry Studies
- Probiotics and Fermented Foods
- Identification and Quantification in Food
- Environmental Chemistry and Analysis
- Surfactants and Colloidal Systems
- Infective Endocarditis Diagnosis and Management
- Fungal and yeast genetics research
- Mycotoxins in Agriculture and Food
- Mycobacterium research and diagnosis
- Ionic liquids properties and applications
University of Perugia
2014-2025
The aim of this study was to assess potential candidate gene regions and corresponding universal primer pairs as secondary DNA barcodes for the fungal kingdom, additional ITS rDNA primary barcode. Amplification efficiencies 14 (partially) targeting eight genetic markers were tested across > 1 500 species (1 931 strains or specimens) outcomes almost twenty thousand (19 577) polymerase chain reactions evaluated. We several well-known that amplify: i) sections nuclear ribosomal RNA large...
We report the preparation and study of properties novel deep eutectic solvents (DESs) formed by zwitterionic trimethylglicine high melting point carboxylic acids. These DESs are liquid at room temperature or temperatures lower than 70 °C do not have chloride any metal ions in their composition. media were characterized terms viscosity, conductivity, density, ionicity (via Walden plots), surface tension thermal stability. The values observed similar to other typical DESs, mixtures resulted...
Preparation, properties and toxicity of room temperature deep eutectic solvents formed by (1<italic>S</italic>)-(+)-10-camphorsulfonic acid aromatic, aliphatic amphiphilic sulfobetaines.
Spectroscopy of soft matter such as biological material suffers from low contrast and resolution. A prototype a new instrument concept that combines Raman Brillouin spectrometry, however, demonstrates superior performance for studying the chemical makeup mechanical properties in variety applications.
Microbes growing onto solid surfaces form complex 3-D biofilm structures characterized by the production of extracellular polymeric compounds and an increased resistance to drugs. The quantification relays currently on a number different approaches techniques, often leading evaluations ability biofilms studied microbial strains. Measures biomass were carried out with crystal violet (CV) direct reading at 405 nm, whereas activity was assessed XTT...
BACKGROUND Protein hydrolysates or hydrolysed proteins (HPs) are high-N organic fertilizers allowing the recovery of by-products (leather meal and fluid proteins) otherwise disposed as polluting wastes, thus enhancing matter energy conservation in agricultural systems while decreasing potential pollution. Chemical biological characteristics HPs animal origin were analysed this work to assess their safety, environmental sustainability efficacy fertilizers. Different obtained by thermal,...
Surfactants are extremely important agents to clean and sanitize various environments. Their biocidal activity is a key factor determined by the interactions between amphiphile structure target microbial cells. The object of this study was analyze four structural variants N-alkyltropinium bromide surfactants with Gram negative Escherichia coli positive Listeria innocua bacteria. Microbiological conductometric methods previously described FTIR bioassay were used assess metabolomic damage...
Yeast taxonomy was introduced based on the idea that physiological properties would help discriminate species, thus assuming a strong link between physiology and taxonomy. However, instability of characteristics within species configured them as not ideal markers for delimitation, shading importance paving way to DNA-based The hypothesis reconnecting with specific traits from phylogenies has been successfully explored Bacteria Archaea, suggesting similar route can be traveled yeasts. In this...
This study aimed to evaluate the intrinsic in vitro performance of naïve porcine prepubertal Sertoli cells (SCs) and SCs loaded with blank poly(lactic acid) microparticles (MP) or amphotericin B (AmB-MP) against Candida tropicalis, a prevalent pathogenic non-albicans species. The objective was assess their impact on biofilm formation cellular response mechanisms involved, building previous findings that highlight SCs’ potential as anti-infective agents drug carriers. Our results demonstrated...
During an investigation of olive oil microbiota, three yeast strains were found to be divergent from currently classified species according the sequences D1/D2 domain gene encoding rRNA large subunit (LSU) and internal transcribed spacer region including for 5.8S rRNA. Phylogenetic analysis revealed that these strains, designated CBS 12509, 12510(T) 12511, represent a novel anascosporogenous described herein as Yamadazyma terventina sp. nov; type strain is DAPES 1924(T) (= = NCAIM...
The rapid and accurate identification of pathogen yeast species is crucial for clinical diagnosis due to the high level mortality morbidity induced, even after antifungal therapy. For this purpose, new rapid, high-throughput reliable methods are required. In work we described a combined approach based on two techniques in order improve pathogenic strains. Next Generation Sequencing (NGS) ITS D1/D2 LSU marker regions together with FTIR spectroscopy were applied identify 256 strains belonging...
Two hundred seventy seven strains from eleven opportunistic species of the genus Candida, isolated two Italian hospitals, were identified and analyzed for their ability to form biofilm in laboratory conditions. The majority Candida albicans formed while among NCAC there different level forming ability, accordance with current literature. relation between variables considered, i.e. departments hospitals or biofilm, was tested assessment probability associated each combination. Species...
Species identification of yeasts and other Fungi is currently carried out with Sanger sequences selected molecular markers, mainly from the ribosomal DNA operon, characterized by hundreds tandem repeats 18S, ITS1, 5.8S, ITS2 LSU loci. The ITS region has been recently proposed as a primary barcode marker making this most used one in taxonomy, phylogeny diagnostics. introduction NGS providing tools high efficacy relatively low cost to amplify two or more markers simultaneously great sequencing...
Fourier Transform InfraRed spectroscopy (FTIR) is an emerging technique in biology. One of the analytical problems inherent with this approach extreme variability typical biological systems. Extrinsic fonts variations are growth media and phase microbial culture. In order to gain knowledge on relations between these two factors spectral variability, metabolomic changes Saccharomyces cerevisiaecells during were analyzed FTIR spectroscopy. Spectral reflected occurring all cellular compartments...