A5080306057- Seismic Waves and Analysis
- Microbial Community Ecology and Physiology
- Mycorrhizal Fungi and Plant Interactions
- Soil Carbon and Nitrogen Dynamics
- Plant-Microbe Interactions and Immunity
- Gut microbiota and health
- Legume Nitrogen Fixing Symbiosis
- Plant Pathogens and Fungal Diseases
- Forest Ecology and Biodiversity Studies
- Plant nutrient uptake and metabolism
- Microbial bioremediation and biosurfactants
- Nematode management and characterization studies
- Genomics and Phylogenetic Studies
- Ecology and Vegetation Dynamics Studies
- Species Distribution and Climate Change
- Bioenergy crop production and management
- Evolution and Genetic Dynamics
- Soybean genetics and cultivation
- Fungal Biology and Applications
- Biofuel production and bioconversion
- Peatlands and Wetlands Ecology
- Regional Economic and Spatial Analysis
- Plant Parasitism and Resistance
- Environmental DNA in Biodiversity Studies
- Reproductive tract infections research
Pennsylvania State University
2018-2025
The Scarborough Hospital
2023-2025
University of Toronto
1998-2025
Cornell University
2016-2021
Université de Montréal
2013-2021
Espace pour la vie
2013-2021
McGill University
2011-2015
National Research Council Canada
2011-2013
National Academies of Sciences, Engineering, and Medicine
2013
Biotechnology Research Institute
2011
Bioremediation is a cost-effective and sustainable approach for treating polluted soils, but our ability to improve on current bioremediation strategies depends isolate microorganisms from these soils. Although culturing widely used in research applications, it unknown whether the composition of cultured isolates closely mirrors indigenous microbial community contaminated To assess this, we paired culture-independent (454-pyrosequencing total soil DNA) with culture-dependent (isolation using...
Soil microbial responses to climate warming in temperate regions may interact with the effects of increased atmospheric N deposition. In addition, combined these factors on activity during plant growing season differ from over winter, when reduced soil C inputs and freezing can alter nutrient availability demand. We examined seasonal changes extracellular enzyme (EEA), biomass N, fungal bacterial content a addition experiment old field. For EEA, we both hydrolases (organic degrading enzymes,...
ABSTRACT Arctic soils are increasingly susceptible to petroleum hydrocarbon contamination, as exploration and exploitation of the increase. Bioremediation in these is challenging due logistical constraints because soil temperatures only rise above 0°C for ∼2 months each year. Nitrogen often added contaminated situ stimulate existing microbial community, but little known about how nutrients used by microorganisms. Microbes vary widely their ability metabolize hydrocarbons, so question...
Plants interact closely with microbes, which are partly responsible for plant growth, health, and adaptation to stressful environments. Engineering the plant-associated microbiome could improve survival performance in environments such as contaminated soils. Here, willow cuttings were planted into highly petroleum-contaminated soils that had been gamma-irradiated subjected one of four treatments: inoculation rhizosphere soil from a grew well (LA) or sub-optimally (SM) bulk died (DE) no (CO)....
Canola is one of the most economically important crops in Canada, and root rhizosphere microbiomes a canola plant likely impact its growth nutrient uptake. The aim this study was to determine whether has core microbiome (i.e., set microbes that are consistently selected environment), distinct from other commonly grown Canadian Prairies, pea, wheat. We also assessed agronomic treatments can modify microbiome, associated enhanced yield. used field experiment with randomized complete block...
We show that choice of soil microbiome transfer method, i.e. direct transfers and a common wash procedure, dramatically influences the develops in new environment, using high-throughput amplicon sequencing 16S rRNA genes fungal internal transcribed spacer (ITS) region. After 3 weeks incubation commercial potting mix, microbiomes were most similar to source when greater volume initial was transferred (5% v/v transfer), least wash. Abundant operational taxonomic units substantially affected by...
Abstract The microbiome of a vineyard may play critical role in fruit development, and consequently, impact quality properties grape wine. Vineyard management approaches that have directly manipulated the clusters been studied, but little is known about how practices soil microbial pool can influence this dynamic. We examined three under-vine practices: 1) herbicide application, 2) cultivation (vegetation removal), 3) natural vegetation (no removal) Riesling New York over three-year period....
Soil microbiome modification may alter system function, which enhance processes like bioremediation. In this study, we filled microcosms with gamma-irradiated soil that was reinoculated the initial or cultivated bacterial subsets obtained on regular media (REG-M) containing crude oil (CO-M). We allowed 8 weeks for stabilization, added and monoammonium phosphate, incubated another 6 weeks, then measured biodegradation of components, taxonomy, functional gene composition. hypothesized targeted...
Although plants introduced for site restoration are pre-selected specific traits (e.g. trace element bioaccumulation, rapid growth in poor soils), the situ success of these likely depends on recruitment appropriate rhizosphere microorganisms from their new environment. We three willow (Salix spp.) cultivars to a contaminated landfill, and performed soil chemical analyses, plant measurements, Ion Torrent sequencing rhizospheric fungal bacterial communities at 4 16 months post-planting. The...
Abstract Fine roots vary dramatically in their functions, which range from resource absorption to within-plant transport. These differences should alter availability root-associated microorganisms, yet most root microbiome studies involve fine homogenization. We hypothesized that microbial filtering would be greatest the distal roots. To test this, we sampled of six temperate tree species a 23-year-old common garden planting, separating by branching order. Rhizoplane bacterial composition...
A wide range of microbial taxa are active in hydrocarbon-contaminated Arctic soils, and many capable hydrocarbon metabolism. The most effective degraders may not naturally dominate following contamination events, so shifts abundance could potentially increase biodegradation. In this study, we contaminated an soil with diesel used gentamicin vancomycin to inhibit distinct portions the community. We measured loss using gas chromatography, bacterial fungal qPCR, assessed diversity community...
This white paper presents a series of perspectives on current and future phytobiome management, discussed at the Wild Tamed Phytobiomes Symposium in University Park, PA, U.S.A., June 2018. To enhance plant productivity health, to translate lab- greenhouse-based research field applications, academic community end-users need address variety scientific, practical, social challenges. Prior discussion phytobiomes has focused heavily plant-associated bacterial fungal assemblages, but concept...
ABSTRACT The fundamental niches of bacteria can be defined along many environmental axes, including temperature tolerance and resources consumed, while interactions with other organisms constrain (e.g. competition) or enlarge cross-feeding) realized niches. Organisms are often categorized as generalists specialists, corresponding to broad narrow niche requirements, which then linked their functional role in an ecosystem. We show how these terms applied bacteria, make predictions about the...
Host genetic variation can shape the diversity and composition of associated microbiomes, which may reciprocally influence host traits performance. While basis phenotypic plant populations in nature has been studied, comparatively little research investigated genetics effects on their microbiomes. Switchgrass (Panicum virgatum) is a highly outcrossing, perennial, grass species with substantial locally adaptive across its native North American range. Here, we compared 383 switchgrass...
Phytoremediation is a potentially inexpensive alternative to chemical treatment of hydrocarbon-contaminated soils, but its success depends heavily on identifying factors that govern the root-associated microorganisms involved in hydrocarbon degradation and plant growth stimulation. Arbuscular mycorrhizal fungi (AMF) form symbioses with many terrestrial plants, are known stimulate growth, although both species identity environment influence this relationship. Although AMF suspected play role...
Efficient use of managed land depends on our ability to optimize relevant processes (e.g. crop growth) in that space. Microbial activities are critical this goal, given their enormous contributions biogeochemical flux and organismal health. Unfortunately, we still cannot predictably harness potential the same way can introduce nutrients or manipulate plant composition, for example. In recent years, iterative microbiome passaging has been investigated as an approach capturing optimizing...
ABSTRACT Inoculation of plant-beneficial microbes into agricultural soils can improve crop growth, but such outcomes depend on microbial survival. Here, we assessed how exposure to prior environmental conditions impacts in-soil fitness, particularly focusing incubation in liquid culture as an unavoidable phase inoculant production and pre-incubation target a potential method performance. We conducted experimental evolution phosphorus-solubilizing bacterial species, Priestia megaterium , (i)...