Abstract Background Mastitis caused by multiple factors remains one of the most common and costly disease dairy industry. Multi-omics approaches enable comprehensive investigation complex interactions between layers information to provide a more holistic view pathogenesis. Therefore, this study investigated genomic epigenomic signatures possible regulatory mechanisms underlying subclinical mastitis integrating RNA sequencing data (mRNA lncRNA), small (miRNA) DNA methylation milk somatic...

Abstract Background DNA methylation has been documented to play vital roles in diseases and biological processes. In bovine, little is known about the regulatory of alterations on production health traits, including mastitis. Results Here, we employed whole-genome sequencing profile patterns milk somatic cells from sixteen cows with naturally occurring Staphylococcus aureus ( S. ) subclinical mastitis ten healthy control cows. We observed abundant alterations, 3,356,456 differentially...

Arbuscular mycorrhizal fungi (AMF) are obligate plant symbionts that improve the nutrition and health of their host. Most, but not all crops form a symbiosis with AMF. It is case for canola (Brassica napus), an important crop in Canadian Prairies known to this association. From 2008 2018, experiment was replicated at three locations it used assess impact on community AMF naturally occurring cropping systems, monoculture, or two different rotation systems (2-years, canola-wheat 3-years,...

Wheat is among the important crops harnessed by humans whose breeding efforts resulted in a diversity of genotypes with contrasting traits. The goal this study was to determine whether different old and new cultivars durum wheat (Triticum turgidum L. var. durum) recruit specific arbuscular mycorrhizal (AM) fungal communities from indigenous AM populations soil under field conditions. A historical set five landraces 26 were cultivated humid climate Eastern Canada, phosphorus-limiting To...

Staphylococcus aureus is one of the most prevalent contagious bacterial pathogen bovine mastitis. The subclinical mastitis it causes has long-term economic implications and difficult to control. To further understanding genetic basis mammary gland defense against S. infection, transcriptomes milk somatic cells from 15 cows with persistent natural infection (S. aureus-positive, SAP) 10 healthy control (HC) were studied by deep RNA-sequencing technology. Comparing SAP HC group revealed 4,077...

Near infrared reflectance spectroscopy (NIRS) holds promise for rapid assessment of soil total N and organic C contents, but its ability to predict supply in soils with contrasting physio-chemical properties needs be evaluated. The objectives this study were to: (i) evaluate NIRS predictions measured parameters (total N, C, C/N ratio) as an indirect indicator supply, namely corn (Zea mays L.) uptake (ii) assess the effect sample set heterogeneity on predictions. Soil samples (n = 282 set)...

Staphylococcus chromogenes (SC) is a common coagulase-negative staphylococcus described as an emerging mastitis pathogen and commonly found in dairy farms. This study investigated the potential involvement of DNA methylation subclinical caused by SC. The whole-genome patterns transcriptome profiles milk somatic cells from four cows with naturally occurring SC (SCM) healthy were characterized next-generation sequencing, bioinformatics, integration analyses. Comparisons revealed abundant...

Abstract Staphylococcus aureus (S. aureus) is an opportunistic pathogen frequently associated with subclinical mastitis and accounts for a large proportion of the economic losses due to on Canadian dairy farms. Despite plethora investigations molecular mechanisms mastitis, little information available roles regulatory noncoding RNAs (ncRNA). This study aimed uncover microRNAs (miRNAs) long (lncRNAs) in response S. cows. Transcriptome sequencing (RNA-Seq miRNA-Seq) was conducted milk somatic...

<title>Abstract</title> Background Mastitis caused by multiple factors remains one of the most common and costly disease dairy industry. Multi-omics approaches enable comprehensive investigation complex interactions between layers information to provide a more holistic view pathogenesis. Therefore, this study investigated genomic epigenomic signatures possible regulatory mechanisms underlying subclinical mastitis integrating RNA sequencing data (mRNA lncRNA), small (miRNA) DNA methylation...

Abstract Staphylococcus chromogenes (SC), a frequent non-aureus staphylococcus on Canadian herds causes both clinical and subclinical forms of mastitis contributes to large economic losses farms. However, little research attention has been directed uncovering the molecular mechanisms underlying SC pathogenesis, including roles small nucleolar RNAs (snoRNAs). SnoRNAs function regulate host ribosomal (rRNAs) biogenesis processing, are known have regulatory functions in several human diseases,...

Abstract Background Mastitis caused by different pathogens including Streptococcus uberis ( S. ) is responsible for huge economic losses to the dairy industry. In order investigate potential genetic and epigenetic regulatory mechanisms of subclinical mastitis due , DNA methylome (whole genome methylation sequencing) transcriptome (RNA milk somatic cells from cows with naturally occurring healthy control (N = 3/group) were studied. Results Globally, levels CpG sites low in promoters first...

Abstract DNA methylation involvement in the regulation of mammary gland inflammatory defense against pathogens, including Staphylococcus aureus(SA) has been documented. However, mechanisms are not clear. The possible influence first introns (DNA-MeFI) on transcriptional activities during subclinical mastitis was studied by profiling methylome (whole genome sequencing) and transcriptome (RNA-sequencing) milk somatic cells from cows(n=15) with SA (SACs) healthy cows (HCs)(n=13), followed...

Abstract Staphylococcus aureus (S. aureus) is one of the most prevalent pathogens bovine subclinical mastitis and a challenging pathogen in dairy production. DNA methylation involvement regulating mammary gland inflammatory defense has been documented. This study adopted whole genome bisulfite sequencing RNA-sequencing technologies to profile genome-wide transcriptome landscapes, respectively, milk somatic cells (MSCs) from 15 cows with S. aureus-induced (SACs) 13 healthy (HCs)....