A5072144853- Milk Quality and Mastitis in Dairy Cows
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Plant tissue culture and regeneration
- CRISPR and Genetic Engineering
- Probiotics and Fermented Foods
- Infant Nutrition and Health
- Microbial Metabolism and Applications
- Chromosomal and Genetic Variations
- Gut microbiota and health
Qingdao Institute of Bioenergy and Bioprocess Technology
2024
Agriculture and Agri-Food Canada
2022-2023
Université Laval
2022-2023
Sherbrooke Research and Development Centre
2022
Nanjing Medical University
2022
Alfalfa (Medicago sativa. L.) is a globally significant autotetraploid legume forage crop. However, despite its importance, establishing efficient gene editing systems for cultivated alfalfa remains formidable challenge. In this study, we pioneered the development of highly effective ultrasonic-assisted leaf disc transformation system Gongnong 1 alfalfa, variety widely in Northeast China. Subsequently, created single transcript CRISPR/Cas9 (CRISPR_2.0) toolkit, incorporating multiplex gRNAs,...
Staphylococcus aureus is one of the most prevalent contagious bacterial pathogen bovine mastitis. The subclinical mastitis it causes has long-term economic implications and difficult to control. To further understanding genetic basis mammary gland defense against S. infection, transcriptomes milk somatic cells from 15 cows with persistent natural infection (S. aureus-positive, SAP) 10 healthy control (HC) were studied by deep RNA-sequencing technology. Comparing SAP HC group revealed 4,077...
Staphylococcus chromogenes (SC) is a common coagulase-negative staphylococcus described as an emerging mastitis pathogen and commonly found in dairy farms. This study investigated the potential involvement of DNA methylation subclinical caused by SC. The whole-genome patterns transcriptome profiles milk somatic cells from four cows with naturally occurring SC (SCM) healthy were characterized next-generation sequencing, bioinformatics, integration analyses. Comparisons revealed abundant...
To investigate the roles of DNA methylation and gene expression during Streptococcus uberis-induced subclinical mastitis, methylome transcriptome profiles milk somatic cells (MSC) from S. uberis positive control cows (n=3/group) were obtained by next-generation sequencing. Integration analysis between MethGET revealed clear changes in levels 585 promoters (pMeDEGs) bodies 424 genes (gbMeDEGs) conjunction with (P-value<0.0005). The significantly enriched immune-related functions (antigen...