A5068758926- Malaria Research and Control
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Parasites and Host Interactions
- Global Maternal and Child Health
- Insect Pest Control Strategies
- Dengue and Mosquito Control Research
- Insect Resistance and Genetics
- Parasitic Infections and Diagnostics
- Vector-borne infectious diseases
- Drug Transport and Resistance Mechanisms
- Primate Behavior and Ecology
- Travel-related health issues
- Insect symbiosis and bacterial influences
- HIV/AIDS drug development and treatment
- Drug-Induced Hepatotoxicity and Protection
- Hemoglobinopathies and Related Disorders
- Identification and Quantification in Food
- Medicinal Plant Research
- SARS-CoV-2 and COVID-19 Research
- Healthcare Quality and Satisfaction
- Complement system in diseases
- Pharmacological Effects of Natural Compounds
- Viral Infections and Vectors
- Diverse Scientific Research Studies
Hasanuddin University
2016-2025
Eijkman Institute for Molecular Biology
2014-2023
Agencia Nacional de Investigación e Innovación
2023
Agence Nationale de la Recherche
2023
National Research and Innovation Agency
2022-2023
Rumah Sakit Umum Pusat Nasional Dr. Cipto Mangunkusumo
2019
Faculty of Public Health
2014
Kasetsart University
2014
Oxford University Clinical Research Unit Indonesia
2012
Nuffield Orthopaedic Centre
2012
Recent gains in reducing the global burden of malaria are threatened by emergence Plasmodium falciparum resistance to artemisinins. The discovery that mutations portions a P. gene encoding kelch (K13)–propeller domains major determinant has provided opportunities for monitoring such on scale.
Infectious diseases exert a constant evolutionary pressure on the genetic makeup of our innate immune system. Polymorphisms in Toll-like receptor 4 (TLR4) have been related to susceptibility Gram-negative infections and septic shock. Here we show that two polymorphisms TLR4, Asp299Gly Thr399Ile, unique distributions populations from Africa, Asia, Europe. Genetic functional studies are compatible with model which nonsynonymous polymorphism has evolved as protective allele against malaria,...
Drug resistance compromises control of malaria. Here, we show that to a commonly used antimalarial medication, atovaquone, is apparently unable spread. Atovaquone pressure selects parasites with mutations in cytochrome b, respiratory protein low but essential activity the mammalian blood phase parasite life cycle. Resistance rescue from drug later prove lethal mosquito phase, where require full respiration. Unable respire efficiently, resistant fail complete development, arresting their...
A cluster-randomized, double-blinded, placebo-controlled trial was conducted to estimate the protective efficacy (PE) of a spatial repellent (SR) against malaria infection in Sumba, Indonesia. Following radical cure 1,341 children aged ≥ 6 months ≤ 5 years 24 clusters, households were given transfluthrin or placebo passive emanators (devices designed release vaporized chemical). Monthly blood screening and biweekly human-landing mosquito catches performed during 10-month baseline (June...
Background Asymptomatic Plasmodium spp. infections and anemia are highly prevalent conditions in tropical regions. We studied whether asymptomatic parasitemia induces hepcidin- and/or cytokine-mediated iron maldistribution anemia.Design Methods A group of 1197 Indonesian schoolchildren, aged 5–15 years, were screened by microscopy for the presence parasitemia. Concentrations hemoglobin, serum hepcidin parameters status inflammation determined at baseline 4 weeks after antimalarial...
Atovaquone, a coenzyme Q analogue has been indicated to specifically target the cytochrome bc1 complex of mitochondrial respiratory chain in malarial parasite and other protozoan. Various mutations quinone binding site b gene Plasmodium spp. such as M133I, L144S, L271V, K272R, Y268C, Y268S, Y268N, V284F are suggesting associate with resistance atovaquone. There is no direct evidence relation between atovaquone that available. Technical difficulties isolating active assayable mitochondria...
Background The range of the Asian tiger mosquito Aedes albopictus is expanding globally, raising threat emerging and re-emerging arbovirus transmission risks including dengue chikungunya. Its detection in Papua New Guinea's (PNG) southern Fly River coastal region 1988 1992 placed it 150 km from mainland Australia. However, was not until 12 years later that appeared on Torres Strait Islands. We hypothesized extant PNG population expanded into Straits as an indirect effect drought-proofing...
A randomized, double-blinded, placebo-controlled study was conducted to examine the effect of spatial repellent (SR) in households at risk malaria Indonesia. Following presumptive radical cure for 180 adult men representing sentinels new infection four clusters within two villages, all were given either metofluthrin or placebo mosquito coils. Weekly blood smear screening and human-landing catches done throughout 6 months intervention. Malaria infections occurred 61 subjects living 31 SR coil...
The goal to eliminate malaria from the Asia-Pacific by 2030 will require safe and widespread delivery of effective radical cure malaria. In October 2017, Asia Pacific Malaria Elimination Network Vivax Working Group met discuss impediments primaquine (PQ) cure, how these can be overcome methodological difficulties in assessing clinical effectiveness cure. salient discussions this meeting which involved 110 representatives 18 partner countries 21 institutional organizations are reported....
Within the last century, increases in human movement and globalization of trade have facilitated establishment several highly invasive mosquito species new geographic locations with concurrent major environmental, economic health consequences. The Asian tiger mosquito, Aedes albopictus, is an extremely aggressive daytime-biting that a public threat throughout its expanding range.We used 13 nuclear microsatellite loci (on 911 individuals) mitochondrial COI sequences to gain better...
The emergence of insecticide resistant Aedes aegypti mosquitoes has hampered dengue control efforts. WHO susceptibility tests, using several pyrethroid compounds, were conducted on Ae. larvae that collected and raised to adulthood from Semarang, Surakarta, Kudus Jepara in Java. AaNaV gene fragment encompassing kdr polymorphic sites both susceptible was amplified, polymorphisms associated with the phenotype. tests demonstrated Ae, resistance pyrethroids, mortality rates ranging 1.6%–15.2%....
Malaria is one of the most life-threatening infectious diseases worldwide and claims millions peoples life each year. The appearance drug-resistance Plasmodium falciparum has made treatment malaria increasingly problematic, thus, it a dire need to search new alternatives current drugs. In present study, 44 cassane- norcassane-type diterpenes isolated from Caesalpinia crista Myanmar Indonesia were evaluated for their antimalarial activity against parasite FCR-3/A2 clone in vitro. Most tested...
Adequate responses by our innate immune system toward invading pathogens were of vital importance for surviving infections, especially before the antibiotic era. Recently, a polymorphism in Mal (Ser180Leu, TIRAP rs8177374), an important adaptor protein downstream Toll-like receptor (TLR) 2 and 4 pathways, has been described to provide protection against broad range infectious pathogens. We assessed functional effects this human experimental endotoxemia, we demonstrate that individuals...
A deficiency in ADAMTS13 (a von Willebrand factor [VWF] cleaving protease) is associated with accumulation of prothrombogenic unusually large VWF multimers (UL-VWF) plasma. We studied release and proteolysis patients symptomatic Plasmodium falciparum or P. vivax malaria on the Indonesian island Sumba. Malaria had significantly lower platelet counts higher concentrations activation factors than healthy hospital staff controls. The latter indicates that a amount circulating was conformation...
Dengue disease is currently a major health problem in Indonesia and affects all provinces the country, including Semarang Municipality, Central Java province. While dengue endemic this region, only limited data on epidemiology available. To understand dynamics of Semarang, we conducted clinical, virological, demographical surveillance its surrounding regions 2012. cases were detected both urban rural areas located various geographical features, coastal highland areas. During an eight months'...
Background and Aim: Zoonotic malaria is an emerging public health challenge in Indonesia, exacerbated by deforestation increased interaction between humans non-human primates (NHPs). This study aimed to estimate prevalence NHPs within the Buton Utara Wildlife Sanctuary (BUWS) evaluates potential risk of zoonotic transmission nearby human populations. Materials Methods: Epidemiologic surveys were conducted from 2020 2021 BUWS. Macaca brunnescens, endemic NHP species, captured using traps....
Evaluating long-term immunity after COVID-19 infection and vaccination is critical for managing potential outbreaks. The aim of this study was to develop a cost-effective in-house enzyme-linked immunosorbent assay (ELISA) based on Escherichia coli-expressed SARS-CoV-2 spike protein (E-S1) antibody detection evaluate its performance. system validated by comparing the ELISA results with those obtained using commercial HEK293-expressed (H-S1). Recombinant produced in E. coli, purified,...
Evaluating long-term immunity after COVID-19 infection and vaccination is critical for managing potential outbreaks. The aim of this study was to develop a cost-effective in-house enzyme-linked immunosorbent assay (ELISA) based on Escherichia coli-expressed SARS-CoV-2 spike protein (E-S1) antibody detection evaluate its performance. system validated by comparing the ELISA results with those obtained using commercial HEK293-expressed (H-S1). Recombinant produced in E. coli, purified,...