A5101997799- Systemic Sclerosis and Related Diseases
- Immunotherapy and Immune Responses
- Autoimmune Bullous Skin Diseases
- T-cell and B-cell Immunology
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Urticaria and Related Conditions
- Physics of Superconductivity and Magnetism
- Advanced Condensed Matter Physics
- Inflammatory Myopathies and Dermatomyositis
- Magnetic and transport properties of perovskites and related materials
- Immune Cell Function and Interaction
- Malaria Research and Control
- Phosphodiesterase function and regulation
- Eosinophilic Disorders and Syndromes
- Dermatological and Skeletal Disorders
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Dermatology and Skin Diseases
- Skin Diseases and Diabetes
- Psoriasis: Treatment and Pathogenesis
- melanin and skin pigmentation
- Crystallization and Solubility Studies
- Connective Tissue Growth Factor Research
- Protist diversity and phylogeny
- Dermatologic Treatments and Research
- Platelet Disorders and Treatments
Kanagawa Dental University
2002-2023
Otsu Municipal Hospital
2022
The University of Tokyo
2003-2018
Tokyo Medical and Dental University
2018
Mitsubishi Tanabe Pharma Corporation
2018
Nippon Medical School
2011
Hiroshima Kyoritsu Hospital
2010
Nagoya City University
2010
Keio University Hospital
2008
Osaka University
2006
Small, compact genomes of ultrasmall unicellular algae provide information on the basic and essential genes that support lives photosynthetic eukaryotes, including higher plants1,2. Here we report 16,520,305-base-pair sequence 20 chromosomes red alga Cyanidioschyzon merolae 10D as first complete algal genome. We identified 5,331 in total, which at least 86.3% were expressed. Unique characteristics this genomic structure include: a lack introns all but 26 genes; only three copies ribosomal...
Abstract We studied the in vitro effect of testosterone on spontaneous immunoglobulin production by human peripheral blood mononuclear cells (PBMC). Testosterone inhibited IgG and IgM PBMC both from males females. The inhibitory was revealed at doses more than 1 nm, increased dose-dependently, reached a plateau 100 nm. At <1000 did not reduce cell viability. treatment reduced 59.0% that 61.3% compared with control. Immunoglobulin B also suppressed testosterone, though magnitude...
High-energy spin excitations up to above 8000 ${\mathrm{cm}}^{\mathrm{\ensuremath{-}}1}$ were measured by polarized Raman scattering in the insulating phases of S=1/2 copper oxides, ${\mathrm{La}}_{2}$${\mathrm{CuO}}_{4}$, ${\mathrm{YBa}}_{2}$${\mathrm{Cu}}_{3}$${\mathrm{O}}_{6.2}$, ${\mathrm{Bi}}_{2}$${\mathrm{Sr}}_{2}$${\mathrm{Ca}}_{0.5}$${\mathrm{Y}}_{0.5}$${\mathrm{Cu}}_{2}$${\mathrm{O}}_{8+\mathit{y}}$, ${\mathrm{Nd}}_{2}$${\mathrm{CuO}}_{4}$, and...
Ultraviolet (uv) radiation has profound effects on the immune system both in vitro and vivo. Recent studies, utilizing uv irradiation of intact animals, have focused suppressive effect generation allergic contact sensitization (ACS). To explore mechanism(s) by which affects ACS, we used a recently described technique sensitizing mice with subcutaneous (s.c.) injection haptenated epidermal cells. uv-treated or untreated mouse cells (EC) were conjugated 1 mM trinitrobenzene sulfonate injected...
Atovaquone, a coenzyme Q analogue has been indicated to specifically target the cytochrome bc1 complex of mitochondrial respiratory chain in malarial parasite and other protozoan. Various mutations quinone binding site b gene Plasmodium spp. such as M133I, L144S, L271V, K272R, Y268C, Y268S, Y268N, V284F are suggesting associate with resistance atovaquone. There is no direct evidence relation between atovaquone that available. Technical difficulties isolating active assayable mitochondria...
Atopic dermatitis (AD) is a chronic and relapsing inflammatory skin disease characterized by the predominant infiltration of T cells, eosinophils macrophages in lesional skin. Recently, eotaxin-2/CCL24 eotaxin-3/CCL26 were identified as CC chemokines that signal exclusively via CCR3 receptor have eosinophil-selective chemoattractant activity, does eotaxin/CCL11. We previously reported serum levels thymus activation-regulated chemokine (TARC)/CCL17 macrophage-derived (MDC)/CCL22 correlated...
Bullous pemphigoid (BP) often provokes blood and tissue eosinophilia, which suggests that some chemoattractants modulate the eosinophil infiltration in BP. Eotaxin, a CC chemokine, strongly attracts eosinophils, interleukin (IL)-5 induces differentiation, proliferation colony formation vitro.To examine correlation between levels of eotaxin IL-5 number lesional expression BP lesions.In this study we measured blister fluid by enzyme-linked immunosorbent assay. We also examined lesions...
No AccessJournal of UrologyInvestigative Urology1 Aug 2012Avanafil, a Potent and Highly Selective Phosphodiesterase-5 Inhibitor for Erectile Dysfunction Jun Kotera, Hideki Mochida, Hirotaka Inoue, Tsunehisa Noto, Kotomi Fujishige, Takashi Sasaki, Tamaki Kobayashi, Koki Kojima, Shiyin Yee, Yasuhiro Yamada, Kohei Kikkawa, Kenji Omori KoteraJun Kotera Advanced Medical Research Laboratories, Mitsubishi Tanabe Pharma Corp., Yokohama, Japan , MochidaHideki Mochida Pharmacology Laboratories II,...
To determine the prevalence and characterization of antibodies to endothelial cells in patients with SSc, serum samples from 80 20 systemic lupus erythematosus (SLE), healthy control subjects were examined by ELISA using cultured human umbilical vein (HUVEC), indirect immunofluorescence analysis (IIF), immunoblotting cytoplasmic extract HUVEC. IgG and/or IgM isotype anti-endothelial cell (AECA) demonstrated 43 SSc (54%), 15 SLE (75%), none subjects. Immunofluorescence on HUVEC substrate...
Chloroplast DNA (cpDNA) is packed into discrete structures called chloroplast nucleoids (cp-nucleoids). The structure of cpDNA thought to be important for its maintenance and regulation. In bacteria mitochondria, histone-like proteins (such as HU Abf2, respectively) are abundant play roles in organization. However, a primary structural protein has yet found cp-nucleoids. Here, we identified an binding from isolated cp-nucleoids the primitive red alga Cyanidioschyzon merolae. purified had...
Objective. Pulmonary hypertension (PH) is a frequent cause of death in patients with systemic sclerosis (SSc). In this study, we examined the occurrence PH and investigated clinical laboratory features SSc PH. Methods. A cross‐sectional study 125 Japanese was conducted using Doppler echocardiography, other multiple cardiopulmonary tests, examination. Results. (systolic pressure >40 mmHg) diagnosed 20 (16%) by echocardiography. six who had secondary pulmonary (SPH), due to severe fibrosis; 14...
This is the first report of molecular characterization a novel cyclic nucleotide PDE (phosphodiesterase), isolated from human malaria parasite Plasmodium falciparum and designated PfPDE1. PfPDE1 cDNA encodes an 884-amino-acid protein, including six putative transmembrane domains in N-terminus followed by catalytic domain. The gene single-copy consisting two exons 170 bp intron. transcripts were abundant ring form asexual blood stages parasite. C-terminal domain PfPDE1, produced Escherichia...
Antiferromagnetic magnons ${\mathrm{Nd}}_{2}$${\mathrm{CuO}}_{4}$, which is the mother material of electron superconductor (${\mathrm{Nd}}_{1\mathrm{\ensuremath{-}}\mathrm{x}}$${\mathrm{Ce}}_{\mathrm{x}}$${)}_{2}$${\mathrm{CuO}}_{4\mathrm{\ensuremath{-}}\mathrm{y}}$, are observed by Raman scattering. The effective exchange integral estimated from two-magnon peak energy 1070 ${\mathrm{cm}}^{\mathrm{\ensuremath{-}}1}$, unexpectedly large in spite Cu-Cu atomic distance compared with those other...
BAFF [B-cell activating factor belonging to the tumour necrosis (TNF) family] is a member of TNF superfamily that regulates B-lymphocyte proliferation and survival. It has been demonstrated increased levels soluble are associated with systemic autoimmunity in patients lupus erythematosus, rheumatoid arthritis Sjögren's syndrome, animal models spontaneous autoimmune diseases. However, significance circulating bullous diseases unknown.To examine whether elevated blistering pemphigus vulgaris...
CD83 is a member of the Ig superfamily expressed primarily by mature dendritic cells (DCs). In mice, expression thymic stromal regulates CD4+ T cell development, with CD83−/− mice demonstrating dramatic reductions in both thymus and peripheral cells. this study, was also found to affect MHC class II antigen within periphery. deficiency reduced cell-surface 25–50% on splenic B DCs, epithelial peritoneal macrophages. Reduced stable intrinsic property that resulted from increased...
A neutron scattering study on Nd 2 CuO 4 , a mother material of the electron-doped superconductor (Nd 1- x Ce )CuO 4- y was carried out. Reentrant magnetic phase transitions associated with spin reorientation antiferromagnetic ordering were observed. La NiO -type order stabilized in range 80 K\({\lesssim}T{\lesssim}\)250 K and at \(T{\lesssim}40\) K. Between 40 K\({\lesssim}T{\lesssim}\)80 K, realized. Diffuse due to two-dimensional fluctuation also observed around T ≃250
ULTRASOUND MEASUREMENT OF SKIN THICKNESS IN SYSTEMIC SCLEROSIS H. IHN, IHN Department of Dermatology, Faculty Medicine, University TokyoTokyo, Japan Correspondec to Ihn, Division Rheumatology, Medical South Carolina, 171 Ashley Ave. Charleston, SC 29425-2229, USA. Search for other works by this author on: Oxford Academic PubMed Google Scholar M. SHIMOZUMA, SHIMOZUMA FUJIMOTO, FUJIMOTO S. SATO, SATO K. KIKUCHI, KIKUCHI A. IGARASHI, IGARASHI Y. SOMA, SOMA TAMAKI, TAMAKI TAKEHARA Volume 34,...
Summary Background Bullous pemphigoid (BP) is an autoimmune blistering disease characterized by eosinophilia and high serum IgE levels. The accumulated evidence suggests that various cytokines are involved in the lesional skin of patients with BP. Recently, thymus activation‐regulated chemokine (TARC/CCL17), a CC chemokine, was identified as selective chemoattractant for receptor 4 (CCR4)‐expressing cells. Objective In this study, we examined involvement TARC Methods We determined fluid...
Objective. To elucidate the role of transforming growth factor-β (TGF-β)/Smad signalling in increased expression collagen gene systemic sclerosis (SSc) fibroblasts. Methods. Dermal fibroblasts from seven patients with diffuse SSc recent onset and healthy individuals were studied. The levels Smad2, Smad3 Smad4 proteins determined by immunoblotting. phosphorylation interaction Sp1 or p300 analysed using immunoprecipitation. effects overexpression Smad on human α2(I) transcription investigated...
Mitochondrial DNA (mtDNA) is packed into highly organized structures called mitochondrial nucleoids (mt-nucleoids). To understand the organization of mtDNA and overall regulation its genetic activity within mt-nucleoids, we identified characterized a novel packaging protein, termed Glom (a protein inducing agglomeration chromosome), from condensed mt-nucleoids true slime mold, Physarum polycephalum. This could bind to entire package state in vitro. Immunostaining analysis showed that...
Systemic sclerosis (SSc) is a generalized connective tissue disease which characterized by the presence of several autoantibodies. To determine prevalence and antigen specificity anti-U1RNP antibodies (anti-U1RNP) in patients with SSc, serum samples from 223 117 systemic lupus erythematosus (SLE), 18 mixed (MCTD) 40 healthy control subjects were examined indirect immunofluorescent analysis (IIF), double immunodiffusion, immunoblotting using nuclear extract HeLa cells. Eighteen (8%) SSc shown...