A5101686221- Plant Reproductive Biology
- Photosynthetic Processes and Mechanisms
- Glycosylation and Glycoproteins Research
- Monoclonal and Polyclonal Antibodies Research
- Plant Molecular Biology Research
- Malaria Research and Control
- Protist diversity and phylogeny
- Algal biology and biofuel production
- Pancreatic and Hepatic Oncology Research
- HIV Research and Treatment
- Plant and animal studies
- Mosquito-borne diseases and control
- Plant tissue culture and regeneration
- Legume Nitrogen Fixing Symbiosis
- Marine and coastal plant biology
- Gastric Cancer Management and Outcomes
- Pancreatitis Pathology and Treatment
- Carbohydrate Chemistry and Synthesis
- Genomics and Phylogenetic Studies
- Kruppel-like factors research
- Pharmacogenetics and Drug Metabolism
- Peroxisome Proliferator-Activated Receptors
- Vitamin C and Antioxidants Research
- HIV/AIDS drug development and treatment
- Colorectal Cancer Surgical Treatments
Osaka University
2023
Juntendo University
2017-2021
Kyorin University Hospital
2014-2020
Tokyo University of Science
1996-2016
The University of Tokyo
1986-2016
Waseda University
2012-2015
Takeda (Japan)
2006-2015
RIKEN Advanced Science Institute
2009-2014
Graduate School USA
2014
Kyorin University
2005-2013
Small, compact genomes of ultrasmall unicellular algae provide information on the basic and essential genes that support lives photosynthetic eukaryotes, including higher plants1,2. Here we report 16,520,305-base-pair sequence 20 chromosomes red alga Cyanidioschyzon merolae 10D as first complete algal genome. We identified 5,331 in total, which at least 86.3% were expressed. Unique characteristics this genomic structure include: a lack introns all but 26 genes; only three copies ribosomal...
We have isolated and sequenced a novel 11-kDa virucidal protein, named cyanovirin-N (CV-N), from cultures of the cyanobacterium (blue-green alga) Nostoc ellipsosporum. also produced CV-N recombinantly by expression corresponding DNA sequence in Escherichia coli. Low nanomolar concentrations either natural or recombinant irreversibly inactivate diverse laboratory strains primary isolates human immunodeficiency virus (HIV) type 1 as well HIV 2 simian virus. In addition, aborts cell-to-cell...
Griffithsin (GRFT), a novel anti-HIV protein, was isolated from an aqueous extract of the red alga Griffithsia sp. The 121-amino acid sequence GRFT has been determined, and biologically active subsequently produced by expression corresponding DNA in Escherichia coli. Both native recombinant displayed potent antiviral activity against laboratory strains primary isolates T- M- tropic HIV-1 with EC50 values ranging 0.043 to 0.63 nm. also aborted cell-to-cell fusion transmission infection at...
Krüppel-like zinc finger transcription factors (KLFs) play diverse roles during cell differentiation and development in mammals. We have now shown by microarray analysis that expression of the KLF15 gene is markedly up-regulated 3T3-L1 preadipocytes into adipocytes. Inhibition function KLF15, either a dominant negative mutant or RNA interference, both reduced peroxisome proliferator-activated receptor γ (PPARγ) blocked adipogenesis exposed to inducers adipocyte differentiation. However, did...
Chloroplasts have retained the bacterial FtsZ for division, whereas mitochondria lack except in some lower eukaryotes. Instead, mitochondrial division involves a dynamin-related protein, suggesting that chloroplasts system, dynamin-based system replaced during evolution. In this study, we identified novel plant-specific group of dynamins from primitive red alga Cyanidioschyzon merolae. Synchronization chloroplast and immunoblot analyses showed protein (CmDnm2) associates with only division....
The unique double fertilisation mechanism in flowering plants depends upon a pair of functional sperm cells. During male gametogenesis, each haploid microspore undergoes an asymmetric division to produce large, non-germline vegetative cell and single germ that divides once the pair. Despite importance cells plant reproduction, relatively little is known about molecular mechanisms controlling proliferation specification. Here, we investigate role Arabidopsis germline-specific Myb protein DUO...
Abstract In most algae, the chloroplast division rate is held constant to maintain proper number of chloroplasts per cell. By contrast, land plants evolved cell and differentiation systems in which size change along with their respective cellular function by regulation rate. Here, we show that PLASTID DIVISION (PDV) proteins, plant–specific components apparatus, determine division. Overexpression PDV proteins angiosperm Arabidopsis thaliana moss Physcomitrella patens increased but decreased...
The rapid and aggressive spread of artemisinin-resistant Plasmodium falciparum carrying the C580Y mutation in kelch13 gene is a growing threat to malaria elimination Southeast Asia, but there no evidence their other regions. We conducted cross-sectional surveys 2016 2017 at two clinics Wewak, Papua New Guinea (PNG) where we identified three infections caused by mutants among 239 genotyped clinical samples. One these exhibited highest survival rate (6.8%) all parasites surveyed ring-stage...
ABSTRACT The novel antiviral protein cyanovirin-N (CV-N) was initially discovered based on its potent activity against the human immunodeficiency virus (HIV). Subsequent studies identified HIV envelope glycoproteins gp120 and gp41 as molecular targets of CV-N. More recently, mechanistic have shown that certain high-mannose oligosaccharides (oligomannose-8 oligomannose-9) found comprise specific sites to which CV-N binds. Such selective, carbohydrate-dependent interactions may account, at...
ABSTRACT Cyanovirin-N (CV-N), an 11-kDa protein isolated from the cyanobacterium Nostoc ellipsosporum , potently inactivates diverse strains of human immunodeficiency virus type 1 (HIV-1), HIV-2, and simian virus. While it has been well established that viral surface envelope glycoprotein gp120 is a molecular target CV-N, detailed mechanism action further interest. We compared matched native CV-N-treated preparations in panel assays measure replication, assessing successive stages life...
Fibroblast growth factors (FGFs) are important intercellular signaling molecules in developmental processes. Here, we show that FGF10 is secreted by cultured preadipocytes and prevention of inhibits the expression C/EBPβ subsequent differentiation these cells. An active form rescued cells which was blocked. Development white adipose tissue this knockout mice were markedly reduced, ability embryonic fibroblasts derived from to differentiate into adipocytes impaired. Therefore, plays an role...
Abstract Background The predominant mode of HIV‐1 transmission is by heterosexual contact. cervical/vaginal mucosa the main port HIV entry in women. A safe and effective topical microbicide against urgently needed to prevent sexual transmission. Hence, we evaluated griffithsin (GRFT), a 12.7 kDa carbohydrate‐binding protein, both native recombinant GRFT, potently inhibited CXCR4‐and CCR5‐tropic infection vitro . Methods antiviral efficacy GRFT SHIV strains SIVmac251 was assays. We also time...
All flowering plants exhibit a unique type of sexual reproduction called 'double fertilization' in which each pollen tube-delivered sperm cell fuses with an egg and central cell. Proteins that localize to the plasma membrane gametes regulate one-to-one gamete pairing fusion between male female for successful double fertilization. Here, we have identified protein from Lilium longiflorum generative cells using proteomic analysis found is ortholog Arabidopsis DUF679 DOMAIN MEMBRANE PROTEIN 9...
We investigated whether the T7 system of phage display could produce peptide libraries greater diversity than M13 due to differing processes lytic and filamentous morphogenesis. Using a bioinformatics-assisted computational approach, collections random sequences obtained from 12-mer library (X(12)) 7-mer disulfide-constrained (CX(7)C) were analyzed compared with populations New England BioLabs' Ph.D.-12 Ph.D.-C7C libraries. Based on this analysis, constructed have fewer amino acid biases,...
During plastid division, two structures have been detected at the division site in separate analyses. The plastid-dividing ring can be by transmission electron microscopy as (or three) electron-dense rings: an outer on cytosolic face of envelope, occasionally a middle intermembrane space, and inner stromal envelope. FtsZ ring, which plays central role bacterial also is involved believed to descended plastids from cyanobacterial endosymbiosis. relationship between not known, although there...
To evaluate the accuracy of transvaginal ultrasound (US) and gadolinium-enhanced magnetic resonance (MR) imaging in assessment intratumoral structure an adnexal mass.The population comprised 82 patients with mass who underwent both US MR had surgical confirmation. The findings from modalities were compared retrospectively those surgery.The sensitivity, specificity, 100%, 98%, 99% 97%, 46%, 68% for identification solid tissue masses. Transvaginal exhibited many (n = 25) false-positive...
Abstract The ultrasmall unicellular red alga Cyanidioschyzon merolae lives in the extreme environment of acidic hot springs and is thought to retain primitive features cellular genome organization. We determined 16.5-Mb nuclear sequence C. 10D as first complete algal genome. BLASTs annotation results showed that has a mixed gene repertoire plants animals, also implying relationship with prokaryotes, although its photosynthetic components were comparable other phototrophs. green Chlamydomonas...