A5085099174- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Toxoplasma gondii Research Studies
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Vector-borne infectious diseases
- Mosquito-borne diseases and control
- Malaria Research and Control
- Plant Virus Research Studies
- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Autophagy in Disease and Therapy
- Virus-based gene therapy research
- Rabies epidemiology and control
- Virology and Viral Diseases
- Vector-Borne Animal Diseases
- Insect and Pesticide Research
- Parasitic Infections and Diagnostics
- Glycosylation and Glycoproteins Research
- SARS-CoV-2 and COVID-19 Research
- Redox biology and oxidative stress
- SARS-CoV-2 detection and testing
- Diet and metabolism studies
- Venomous Animal Envenomation and Studies
- Proteoglycans and glycosaminoglycans research
Tokyo University of Agriculture and Technology
2021-2025
Nagoya University
2017-2021
Yamaguchi University
2019
Kasetsart University
2019
Central Mindanao University
2019
IPB University
2019
Rajamangala University of Technology Isan
2019
National Institute of Infectious Diseases
2019
The University of Tokyo
2009-2017
Obihiro University of Agriculture and Veterinary Medicine
2013-2017
Abstract During 2016–2018, we conducted surveillance for Japanese encephalitis virus (JEV) in mosquitoes and pigs Japan, Thailand, the Philippines, Indonesia. Phylogenetic analyses demonstrated that our isolates (genotypes Ia, Ib, III, IV) were related to JEV obtained from same regions many years ago. Indigenous strains persist Asia.
Abstract Diet is the primary factor affecting host nutrition and metabolism, with excess food intake, especially high-calorie diets, such as high-fat high-sugar causing an increased risk of obesity related disorders. Obesity alters gut microbial composition reduces diversity causes changes in specific bacterial taxa. Dietary lipids can alter obese mice. However, regulation microbiota energy homeostasis by different polyunsaturated fatty acids (PUFAs) dietary remains unknown. Here, we...
ABSTRACT Toxoplasma gondii CDPK1 (TgCDPK1) was found to be the target of toxoplasmocidal compound 1NM-PP1. When TgCDPK1 mutated at position 128 from glycine methionine, resistance gained. Inhibition gliding motility without inhibition micronemal secretion by 1NM-PP1 suggests a function for in motility.
The raccoon dog (Nyctereutes procyonoides), classified in the order Carnivora within family Canidae, is native to East Asia and widely distributed throughout Japan due its adaptability various environments. Despite close relationship between dogs other animals, viruses infecting have not been thoroughly investigated Japan. In this study, we performed metatranscriptomic analyses using fecal samples collected from latrines of wild two locations on mainland Nearly complete viral genomes were...
Lumpy skin disease virus (LSDV) affects cattle and causes significant economic damage. The live vaccine derived from an attenuated strain is effective but associated with mild lesions in some vaccinated cattle. Moreover, recombinant LSDV strains, particularly one wild-type field have recently emerged spread throughout Asian countries. A cost-effective typing method required. We developed a multi-locus real-time PCR high-resolution melting (HRM) assay to differentiate between the wild-type,...
Erythrocyte invasion is critical to the pathogenesis and survival of malarial parasite, Plasmodium falciparum. This process partly mediated by proteins that belong Duffy binding-like family, which are expressed on merozoite surface. One these proteins, BAEBL (also known as EBA-140), thought bind glycophorin C in a sialic acid-dependent manner. In this report, binding assay between recombinant protein enzyme-treated erythrocytes, we show erythrocytes primarily acid partially through heparan...
Background cAMP-dependent protein kinase (PKA) has been implicated in the asexual stage of Toxoplasma gondii life cycle through assaying effect a PKA-specific inhibitor on its growth rate. Since inhibition host cell PKA cannot be ruled out, more precise evaluation role PKA, as well characterization itself, is necessary. Methodology/Principal Finding The inhibitory effects two inhibitors, H89, an ATP-competitive chemical inhibitor, and PKI, substrate-competitive mammalian natural peptide were...
Heparin, a sulfated glycoconjugate, reportedly inhibits the blood-stage growth of malaria parasite Plasmodium falciparum. Elucidation inhibitory mechanism is valuable for developing novel invasion-blocking treatments based on heparin. Merozoite surface protein 1 has been reported as candidate target heparin; however, to better understand molecular mechanisms involved, we characterized molecules that bind heparin during merozoite invasion. Here, show binds only at apical tip and multiple...
Rotavirus C (RVC) is a major cause of diarrhoea in swine, cattle, and humans worldwide. RVC exhibits sequence diversity all 11 genes, especially VP4 VP7, segment-based genotyping has been performed similar to rotavirus A. To date, recombination events have reported A B. However, there are no reports describing gene RVC, except for NSP3 between H. In this study, nine porcine strains identified Japanese pigs were completely sequenced analysed together with sequences from the GenBank database....
Here, we assessed the sulfated derivative of microbial polysaccharide gellan gum and derivatives λ κ-carrageenans for their ability to inhibit Plasmodium falciparum 3D7 Dd2 growth invasion red blood cells in vitro. Growth inhibition was by means flow cytometry after a 96-h exposure inhibitors counting ring parasites 20-h them. Gellan sulfate strongly inhibited modestly both P. Dd2; inhibitory effects exceeded those achieved with native gum. The hydrolyzed λ-carrageenan oversulfated...
Toxoplasma rhoptry neck protein 4 (TgRON4) is a component of the moving junction macromolecular complex that plays central role during invasion. TgRON4 exposed on cytosolic side host cell invasion, but its molecular interactions remain unclear. Here, we identified cellular β-tubulin as binding partner TgRON4, not Plasmodium RON4. Coimmunoprecipitation studies in mammalian cells demonstrated C-terminal 15-kDa region was sufficient for to and 17-kDa proximal C-terminus required β-tubulin....
Toxoplasma gondii is an important food and waterborne pathogen that causes severe disease in immunocompromised patients. Bumped kinase inhibitors (BKIs) have antiparasitic effect on T. tachyzoite growth by targeting calmodulin-domain protein 1 (TgCDPK1). To identify mutations confer resistance to BKIs, chemical mutagenesis was performed, followed selection media containing either 250 or 1000 nM 1NM-PP1. Whole-genome sequence analysis of resistant clones revealed single nucleotide...
A PCR-based survey of hemoprotozoa parasites detected Babesia bigemina, Theileria orientalis and Trypanosoma theileri among cattle water buffalo in Vietnam, a new sp. closely related to ovata was only. In addition, annulata evansi were not both buffalo. Phylogenetic analysis T. MPSP genotypes 3, 5, 7 N3 7, N1 N2 Additionally, buffalo-derived CATL sequences clustered together with previously reported cattle-derived sequence from Vietnam. This is the first report cattle, genotype
Heparan and chondroitin sulfates play essential roles in growth factor signaling during development share a common linkage tetrasaccharide structure, GlcAbeta1,3Galbeta1,3Galbeta1,4Xylbeta1-O-Ser. In the present study, we identified Drosophila proteoglycan UDP-galactose:beta-xylose beta1,4-galactosyltransferase I (dbeta4GalTI), determined its substrate specificity. The enzyme transferred Gal to -beta-xylose (Xyl) residue, confirming it be ortholog of human I. Then established...
Toxoplasma gondii is an intracellular parasite that invades nucleated cells, causing toxoplasmosis in humans and animals worldwide. The extremely wide range of hosts susceptible to T. thought be the result interactions between ligands receptors on its target cells. In this study, a host cell-binding protein from was characterized, one identified. P104 (GenBank Access. No. CAJ20677) 991 amino acids length, containing putative 26 acid signal peptide 10 PAN/apple domains, shows low homology...
In the present study, we investigated whether Plasmodium falciparum 1‐Cys peroxiredoxin (Prx) (Pf1‐Cys‐Prx), a cytosolic protein expressed at high levels during haem‐digesting stage, can act as an antioxidant to cope with oxidative burden of haem (ferriprotoporphyrin IX; FP). Recombinant Pf1‐Cys‐Prx (rPf1‐Cys‐Prx) competed glutathione (GSH) for FP and inhibited degradation by GSH. When rPf1‐Cys‐Prx was added GSH‐mediated degradation, amount iron released reduced 23% reaction without ( P <...
Abstract Cryptosporidium parvum is an apicomplexan parasite that can cause serious watery diarrhea, cryptosporidiosis, in human and other mammals. C. invades gastrointestinal epithelial cells, which have abundant glycosaminoglycans on their cell surface. However, little known about the interaction between glycosaminoglycans. In this study, we assessed inhibitory effect of sulfated polysaccharides invasion host cells identified ligands interact with polysaccharides. Among five tested, heparin...
Infectious diseases are an important issue in the poultry industry, requiring early diagnosis and countermeasures. To address this, we present a system based on TaqMan real-time PCR to detect pathogen genome specimens collected from chickens. We designed 12 primer-probe sets for pathogens causing respiratory or systemic symptoms. In field samples, detected three viruses, including DNA RNA bacteria. The chicken anemia virus Avibacterium paragallinarum were only young laying hens,...
Heparan sulfate proteoglycan plays an important role in developmental processes by modulating the distribution and stability of morphogens Wingless, Hedgehog, Decapentaplegic. chondroitin sulfates share a common linkage tetrasaccharide structure, GlcAbeta1,3Galbeta1,3Galbeta1,4Xylbeta-O-Ser. In present study, we identified Drosophila galactosyltransferase II (dbeta3GalTII), determined its substrate specificity, performed functional analysis using RNA interference (RNAi) mutant flies. The...