A5090430304- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Insect and Pesticide Research
- Plant Parasitism and Resistance
- Insect Pest Control Strategies
- Toxin Mechanisms and Immunotoxins
- Mosquito-borne diseases and control
- Phytochemistry and Biological Activities
- Natural product bioactivities and synthesis
- Malaria Research and Control
- Phytochemistry and Bioactive Compounds
- Yersinia bacterium, plague, ectoparasites research
- Agricultural pest management studies
- Insect and Arachnid Ecology and Behavior
- Trypanosoma species research and implications
- Plant Virus Research Studies
- Rabies epidemiology and control
- Insect Resistance and Genetics
- Insect symbiosis and bacterial influences
- Research on Leishmaniasis Studies
- Phytochemical compounds biological activities
- Parasitic Infections and Diagnostics
- Heat shock proteins research
- Poxvirus research and outbreaks
Mongolian University of Life Sciences
2015-2024
Obihiro University of Agriculture and Veterinary Medicine
2014-2023
Bovine babesiosis, mainly caused by Babesia bovis and B. bigemina, is a huge threat to the livestock industry. In Indonesia, current distribution of disease unknown due lack scientific study.In present study, 487 blood samples were collected from cattle with different breeding age groups in broad geographical area across archipelago. The presence antibodies infections bigemina determined using enzyme-linked immunosorbent assay (ELISA), immunochromatographic test (ICT), nested PCR (nPCR)...
Theileria annulata is a haemoprotozoan parasite that causes cancer-like illness known as tropical theileriosis in cattle. In the course of analyzing genetic diversity T. Sri Lanka, we observed merozoite-piroplasm surface antigen (tams1) and protein (tasp)-like gene sequences obtained from bovine blood DNA samples, which were PCR-positive for annulata, conserved but shared low identity with GenBank sequences. Moreover, 18S rRNA Lankan samples contained ten unique single-nucleotide...
The present study evaluated the growth-inhibitory effects of clofazimine, currently used for treating leprosy, against Babesia bovis, B. bigemina, caballi, and Theileria equi in vitro culture microti mice. 50% inhibitory concentrations (IC50s) clofazimine growth T. were 4.5, 3, 4.3, 0.29 μM, respectively. In mice infected with microti, treatment 20 mg/kg body weight administered orally resulted a significantly lower peak parasitemia (5.3%) than that control group (45.9%), which was...
Bovine babesiosis is a serious threat to the cattle industry. We prepared blood DNA samples from 13 with clinical Badulla (n = 8), Jaffna 3), and Kilinochchi 2) districts in Sri Lanka. These tested positive PCR assays specific for Babesiabovis 9), Babesia bigemina Babesiaovata 1). Twelve were B. bovis and/or One cow was negative species but on microscopic examination; phylogenetic positions of 18S rRNA cytochrome oxidase subunit III gene sequences suggested that infected sp. Mymensingh,...
Eight new flavonoid-based 3′-O-β-d-glucopyranosides (1–8) and three galloyl glucosides (9, 11, 12), were isolated from the aerial parts of Saxifraga spinulosa, along with 25 known compounds. The structures compounds elucidated by spectroscopic methods. Most exhibited potent DPPH radical-scavenging activities. Further, their inhibitory activities evaluated against Babesia bovis, bigemina, caballi, Theileria equi, protozoan parasites that cause piroplasmosis in livestock. results indicated...
Heat shock protein 90 (Hsp90) is a chaperone that stabilizes cells during stress or non-stress responses. Previous reports have shown Hsp90 potential drug target to suppress the multiplication of several protozoan parasites. In this study, 17-dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG), an inhibitor, was evaluated for its inhibitory effect on five in vitro cultures Babesia and Theileria species, including B. bovis, bigemina, divergens, caballi, T. equi, microti–infected mouse...
Abstract Background Babesiosis represents a veterinary and medical threat, with need for novel drugs. Artemisinin-based combination therapies (ACT) have been successfully implemented malaria, human disease caused by related parasites, Plasmodium spp. The aim of this study was to investigate whether ACT is active against Babesia in vitro vivo . Methods Mefloquine, tafenoquine, primaquine, methylene blue lumefantrine, alone or artesunate, were tested bovis Parasite growth verified using SYBR...
Recently, we reported that clofazimine (CF) has an anti-piroplasm activity, but it could not completely eliminate parasites in the host. The currently available drug, diminazene aceturate (DA), sometimes been to have toxic side effects. In present study, evaluated combination treatment with CF and DA against piroplasms both vitro vivo. Additionally, mRNA level DNA amounts were analyzed CF‒ DA‒treated Babesia bovis by a qPCR. CF-DA had additive effects on bovis, B. bigemina, caballi...
Diminazene aceturate (DA) and imidocarb dipropionate are commonly used in livestock as antipiroplasm agents. However, toxic side effects common animals treated with these two drugs. Therefore, evaluations of novel therapeutic agents high efficacy against piroplasm parasites low toxicity to host paramount importance. In this study, the 400 compounds Pathogen Box provided by Medicines for Malaria Venture foundation were screened Babesia bovis, bigemina, caballi, Theileria equi. A...
Diminazene aceturate (DA) is commonly used in the treatment of bovine babesiosis caused by Babesia bovis. In this study, we attempted to develop resistance B. bovis vitro DA and clofazimine (CF, a novel antibabesial agent) using short- long-term drug pressures. short term, found that 6.7 ± 2 (0.54 0.16 μM)-, 12.9 8.6 (1.05 0.7 14 5.9 (1.14 0.48 μM)-fold increases half-maximal inhibitory concentration (IC50) were demonstrated on cultivated with 0.04 μM pressure for 4, 8, 12 days,...
Abstract Background The recently discovered Babesia sp. Mymensingh, which causes clinical bovine babesiosis, has a wide geographical distribution. We investigated the phylogenetic position of Mymensingh using its mitochondrial, plastid, and nuclear genes. Based on morphological molecular data, is unique species we named it as naoakii n. Methods A blood DNA sample from Mymensingh-infected cow was subjected to genome sequencing obtain sequences Six trees were then constructed with (1)...
Bovine babesiosis represents a serious threat to the cattle industry in tropics and subtropics. Although several Babesia species infect cattle, only B. bovis, bigemina divergens are known cause clinical babesiosis. However, our recent study demonstrated that newly discovered sp. Mymensingh might be virulent capable of causing cattle. The objective this was determine host range geographical distribution on global scale. A total 2,860 archived DNA samples from 2,263 Sri Lanka (n = 672),...
Persistent and relapsing babesiosis caused by Babesia microti often occurs in immunocompromised patients, has been associated with resistance to antimicrobial agents such as atovaquone. Given the rising incidence of United States, novel drugs are urgently needed. In current study, we tested whether clofazimine (CFZ), an antibiotic used treat leprosy drug-resistant tuberculosis, is effective against B. microti.Mice severe combined immunodeficiency were infected 107B. microti-infected...
Although vaccines are one of the environmentally friendly means to prevent spread ticks, there is currently no commercial vaccine effective against Haemaphysalis longicornis ticks. In this study, we identified, characterized, localized, and evaluated expression patterns, tested immunogenic potential a homologue Rhipicephalus microplus ATAQ in H. (HlATAQ). HlATAQ was identified as 654 amino acid-long protein present throughout midgut Malpighian tubule cells containing six full partial...