A5019462583- Vector-borne infectious diseases
- Insect Pest Control Strategies
- Insect and Pesticide Research
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Malaria Research and Control
- Toxin Mechanisms and Immunotoxins
- Veterinary Equine Medical Research
- Mosquito-borne diseases and control
- Animal Disease Management and Epidemiology
- Medicinal Plant Research
- Insect Resistance and Genetics
- Plant Parasitism and Resistance
- Microbial infections and disease research
- Veterinary medicine and infectious diseases
- Computational Drug Discovery Methods
- Animal health and immunology
- Toxoplasma gondii Research Studies
- Trypanosoma species research and implications
- Coccidia and coccidiosis research
- Veterinary Pharmacology and Anesthesia
- Animal Diversity and Health Studies
- Nigella sativa pharmacological applications
- Antimicrobial Peptides and Activities
- Parasitic Infections and Diagnostics
Mansoura University
2016-2025
Obihiro University of Agriculture and Veterinary Medicine
2015-2024
Hubei University of Arts and Science
2021
This study aimed to survey the prevalence, antimicrobial resistance, and virulence-associated genes of Salmonella enterica recovered from broiler chickens retail shops at El-Sharkia Province in Egypt. virulence factors were determined using polymerase chain reaction assays targeting invA, csgD, hilC, bcfC, stn, avrA, mgtC, ompF, sopE1 pefA genes.One hundred tweenty out 420- samples chickens' cloacal swabs, farm environmental samples, freshly dressed whole chicken carcasses positive species....
A rapid and accurate assay for evaluating antibabesial drugs on a large scale is required the discovery of novel chemotherapeutic agents against Babesia parasites. In current study, we evaluated usefulness fluorescence-based determining efficacies compounds bovine equine hemoparasites in vitro cultures. Three different hematocrits (HCTs; 2.5%, 5%, 10%) were used without daily replacement medium. The results high-throughput screening revealed that best HCT was 2.5% parasites 5% Theileria IC50...
Developing new antibabesial drugs with a low toxic effect to the animal and no resistance from Babesia parasites is in urgent demand. In this concern, antimalarial, anticancer antioxidant of thymoquinone (TQ), phytochemical compound found plant Nigella sativa, has been reported. Therefore, present study, was evaluated on growth piroplasm parasites. Significant inhibition (P < 0.05) vitro were observed after treatment by TQ IC50 values 35.41 ± 3.60, 7.35 0.17, 0.28 0.016, 74.05 4.55 67.33...
The study investigates the effectiveness of blind and ultrasound-guided epidural injections in Egyptian donkeys sacrococcygeal region, a topic limited research, by comparing assessing their onset 20 healthy adult both sexes. In first group, we used ten cadaver that were humanely euthanized. second 10 live, to assess efficacy time analgesia for at region. Cadavers randomly designated (n = 5) or US-guided region sternal recumbency using Methylene Blue (1%) as diagnostic marker caudal...
Aim: This study aimed to evaluate the inhibitory effects of fluoroquinolone antibiotics, including enrofloxacin, enoxacin, trovafloxacin, norfloxacin, and ofloxacin, on in vitro vivo growth Babesia divergens microti parasites, respectively. Materials methods: The antibiotics against B. , respectively were evaluated using fluorescence-based assay. Additionally, combination therapies highly effective (enrofloxacin, trovafloxacin) with diminazene aceturate, luteolin, or pyronaridine...
Introduction Osteoporosis, particularly postmenopausal osteoporosis, is a significant global health challenge with limited treatment options due to severe side effects associated the long-term use of conventional therapies. Therefore, this study aims provide potentially novel therapeutic approach by examining olive oil's on bone mineral density (BMD), biochemical markers, biomechanical properties, and histopathological changes in an ovariectomized (OVX) rat model. Methods In study,...
ABSTRACT In vitro evaluation of chemotherapeutic agents against Babesia and Theileria parasites has become routine, the effectiveness these chemicals is usually determined by comparing parasitemia dynamics untreated treated parasites. Although microscopy widely used to calculate parasitemia, several disadvantages are associated with this technique. The present study evaluated a fluorescence-based method using SYBR green I stain (SG I) screen antibabesial in cultures bovis . linearity between...
Abstract In this study, we evaluated the validity of a fluorescence-based assay using SYBR Green I (SG I) stain for screening antibabesial compounds against B. microti in mice. Two different hematocrits (HCTs; 2.5% and 5%) were used. Correlating relative fluorescence units (RFUs) with parasitemia showed significant linear relationships R 2 values 0.97 0.99 at HCTs 5%, respectively. Meanwhile, Z′ factors high-throughput (HTS) within permissible limit (≥0.5) HCT lower than value 5% HCT. Taken...
Heat shock protein 90 (Hsp90) is a chaperone that stabilizes cells during stress or non-stress responses. Previous reports have shown Hsp90 potential drug target to suppress the multiplication of several protozoan parasites. In this study, 17-dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG), an inhibitor, was evaluated for its inhibitory effect on five in vitro cultures Babesia and Theileria species, including B. bovis, bigemina, divergens, caballi, T. equi, microti–infected mouse...
Recently, we reported that clofazimine (CF) has an anti-piroplasm activity, but it could not completely eliminate parasites in the host. The currently available drug, diminazene aceturate (DA), sometimes been to have toxic side effects. In present study, evaluated combination treatment with CF and DA against piroplasms both vitro vivo. Additionally, mRNA level DNA amounts were analyzed CF‒ DA‒treated Babesia bovis by a qPCR. CF-DA had additive effects on bovis, B. bigemina, caballi...
The present experimental study was conducted for the assessment of efficacy in vitro inhibition myrrh oil on propagation Babesia bovis, B. divergens, bigemina, Theileria equi, and caballi vivo microti mice through fluorescence assay based SYBR green I. culture divergens bovis used to evaluate possible interaction between other commercial compound, such as pyronaridine tetraphosphate (PYR), diminazene aceturate (DA), or luteolin. Nested-polymerase chain reaction protocol using primers...
Since the last survey on gastrointestinal (GIT) parasites infecting dogs in Dakahlia governorate, Egypt, was published 40 years ago, present study detected various GIT feces of 78 stray this governorate. Twenty-one (35.9%) had eggs/oocysts eight different including Toxocara canis (19.2%), Toxascaris leonina (2.6%), hookworms (1.3%), Taenia species (5.1%), Dipylidium caninum Cystoisospora ohioensis and Neospora caninum-like oocysts (1.3%). These results were combined meta-analyses with...
The Xinjiang Uygur Autonomous Region (Xinjiang) borders eight countries and has a complex geographic environment. There are almost 45.696 million herded sheep in Xinjiang, which occupies 13.80% of China’s farming industry. However, there is scarcity reports investigating the role or ticks transmitting tick-borne diseases (TBDs). A total 894 (298 tick pools) were collected from southern Xinjiang. Out 298 pools investigated this study, Rhipicephalus turanicus (Rh. turanicus) Hyalomma...
To use two diagnostic antigens belonging to the frequently associated in Theileria domain, equi (T. equi) protein 82 (Te 82) and T. 104 kDa microneme-rhoptry antigen precursor 43), diagnose infection horses as compared with merozoite antigen-2 (EMA-2). In current study, we applied a cocktail-ELISA containing (EMA-2 + Te either experimentally infected or field infection. Our findings have revealed that cocktail formula of EMA-2 provided more practical sensitive candidate for diagnosing 43...