A5027161141- Vector-borne infectious diseases
- Malaria Research and Control
- Trypanosoma species research and implications
- Research on Leishmaniasis Studies
- Mosquito-borne diseases and control
- Vector-Borne Animal Diseases
- Parasites and Host Interactions
- Insect and Pesticide Research
- Insect symbiosis and bacterial influences
- Viral Infections and Vectors
- Parasite Biology and Host Interactions
- Redox biology and oxidative stress
- Heat shock proteins research
- Insect Resistance and Genetics
- Toxin Mechanisms and Immunotoxins
- Parasitic Infections and Diagnostics
- Trace Elements in Health
- Invertebrate Immune Response Mechanisms
- Helminth infection and control
- Agricultural pest management studies
- Toxoplasma gondii Research Studies
- Zoonotic diseases and public health
- Computational Drug Discovery Methods
- Insect Pest Control Strategies
- Plant Virus Research Studies
Obihiro University of Agriculture and Veterinary Medicine
2015-2024
Gifu University
2020
National Center for Global Health and Medicine
2012
Universidad de Panamá
2010
Hokkaido University
2010
Saitama International Medical Center
1999-2008
Japan Science and Technology Agency
2005-2008
National Institute of Infectious Diseases
2008
Osaka University
2008
Research Medical Center
2001
The surfaces of the infected erythrocyte (IE) and merozoite, two developmental stages malaria parasites, expose antigenic determinants to host immune system. We report on surface-associated interspersed genes (surf genes), which encode a novel polymorphic protein family, SURFINs, present both IEs merozoites. A SURFIN expressed in 3D7 SURFIN4.2, was identified by mass spectrometric analysis peptides cleaved off surface live with trypsin. SURFINs are encoded family 10 surf genes, including...
Mitochondrial (mt) genomes from diverse phylogenetic groups vary considerably in size, structure, and organization. The genus Plasmodium, causative agent of malaria, the phylum Apicomplexa, has smallest mt genome form a circular and/or tandemly repeated linear element 6 kb, encoding only three protein genes (cox1, cox3, cob). closely related genera Babesia Theileria also have small (6.6 kb) that are monomeric with an organization distinct Plasmodium. To elucidate structural divergence...
T-2307, a novel arylamidine, has been shown to exhibit broad-spectrum antifungal activities against clinically significant pathogens. Here, we evaluated the in vitro and vivo antimalarial activity of T-2307. The 50% inhibitory concentrations (IC₅₀s) T-2307 Plasmodium falciparum FCR-3 K-1 strains were 0.47 0.17 μM, respectively. at 2.5 10 mg/kg body weight/day exhibited blood stage liver parasites rodent malaria models. In conclusion, activity.
This study evaluated the stability of LAMP reagents when stored at 25 degrees C and 37 C, also assessed its detection efficiency on different DNA template preparations. Accordingly, using amplified in vitro cultured T. b. brucei (GUTat 3.1) from day 1 to 15 reagent storage. There were no significant differences (P>0.05) sensitivity among -20 (recommended storage temperature). above-mentioned temperatures serially diluted DNAs (genomic extracted by phenol-chloroform method, FTA card hemolysed...
Theileria orientalis is a benign bovine protozoan parasite that occasionally causes serious economic loss in the livestock industry. We report findings of molecular epidemiological survey T. 94 Vietnamese yellow cattle, 43 water buffaloes, 21 sheep, goats and 85 blood-sucking ticks cattle Thua Thien Hue province Vietnam. The major piroplasm surface protein (MPSP) gene was detected using polymerase chain reaction from 13 (13.8%), 11 buffaloes (25.6%), 1 sheep (4.8%) 9 (10.6%). Phylogenetic...
Background Babesia bovis is an apicomplexan intraerythrocytic protozoan parasite that induces babesiosis in cattle after transmission by ticks. During specific stages of the lifecycle, such as sporozoites Plasmodium falciparum and tachyzoites Toxoplasma gondii, host cells are targeted for invasion using a unique, active process termed "gliding motility". However, it not thoroughly understood how merozoites B. target invade red blood (RBCs), gliding motility has so far been observed parasite....
SUMMARY An enzyme-linked immunosorbent assay ( elisa ) was used to diagnose Corynebacterium (Rhodococcus) equi infection in foals. In tests done with different antigen-extraction procedures (sodium dodecyl sulfate, sodium deoxycholate, polyoxy-ethylene [9] p-tert -octylphenol, [9-10] sonification, homogenization, and heat treatment at 121 C), Tween 20 a satisfactory reactive antigen. Using hyperimmune rabbit sera or infected foal sera, we investigated the specificity sensitivity of antigen...
Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect characterize A. cattle ticks Cebu, Philippines. A total of 12 bovine samples 60 Rhipicephalus (Boophilus) microplus were collected on Cebu Island 2011. 16S rRNA-based screening-PCR DNA sequencing revealed 8 (66.7%) (13.3%) be positive for marginale, 1 tick (1.7%) centrale. Selected further characterized based rRNA...
Gastrointestinal zoonotic helminths of dogs and cats have a public health concern worldwide. We investigated the prevalence gastrointestinal significance in lower Northern Thailand utilized molecular tools for species identification hookworms Opisthorchis viverrini. Fecal samples 197 180 were collected. Overall infection using microscopy was 40.1% 33.9% cats. Helminth found both included hookworms, Spirometra spp., Taenia Toxocara O. viverrini, Strongyloides spp. Trichuris Hookworms most...
Background The presence of animal reservoirs in Schistosoma japonicum infection has been a major obstacle the control schistosomiasis. Previous studies have proven that inclusion measures on reservoir hosts for schistosomiasis contributed to decrease human cases. Animal surveillance should therefore be included strengthen and improve capabilities current serological tests. Methodology/Principal Findings Thioredoxin peroxidase-1 (SjTPx-1) four tandem repeat proteins (Sj1TR, Sj2TR, Sj4TR,...
The present study, conducted in Zambia's Luangwa valley where both animal African trypanosomiasis (AAT) and human (HAT) are endemic, combined the use of microscopy molecular techniques to determine presence trypanosome species cattle, goats tsetse flies.This study was between 2008 2010 Petauke, Chama Isoka districts, north-eastern Zambia. A total 243 36 546 flies, were examined for using microscopy, PCR loop-mediated isothermal amplification (LAMP).There poor agreement among test methods...
Bovine babesiosis is a tick-borne disease caused by several species of Babesia which produce acute and fatal in cattle affect livestock industry worldwide. ovata benign widespread east Asian countries causes anemia, particularly are co-infected with Theileria orientalis. The development genetic manipulation methods necessary to improve our understanding the basic biology protozoan pathogens toward better control disease. Such tools have not been developed for B. ovata, aim this study. In...
Babesia ovata, belonging to the phylum Apicomplexa, is an infectious parasite of bovids. It not associated with manifestation severe symptoms, in contrast other types bovine babesiosis caused by B. bovis and bigemina; however, upon co-infection Theileria orientalis, it occasionally induces exacerbated symptoms. Asymptomatic chronic infection bovines usually observed only for ovata. Comparative genomic analysis could potentially reveal factors involved these distinguishing characteristics;...
We have developed two loop-mediated isothermal amplification (LAMP) assays for specific detection of Trypanosoma cruzi and rangeli based on the 18S ribosomal RNA (rRNA) small nucleolar (snoRNA) genes, respectively. The limit is 100 fg 1 pg T. , respectively, with reactions conducted in 60 minutes. LAMP were used infections comparison polymerase chain reaction (PCR) DNA samples extracted from Rhodnius pallescens bugs collected palm trees Panama. Out a total 52 R. 17 (33%) 14 (27%) -positive...
Schistosomiasis continues to be a public health problem in many tropical and subtropical countries. Improving the diagnostic tools for surveillance monitoring areas that have reached elimination level will help hasten possible of this disease. This study therefore aims develop enzyme-linked immunosorbent assay through use recombinant proteins such as thioredoxin peroxidase-1 (SjTPx-1) four tandem repeat (Sj1TR, Sj2TR, Sj4TR, Sj7TR). Cutoff values were calculated using 38 serum samples from...