A5053871659- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Malaria Research and Control
- Virology and Viral Diseases
- HIV Research and Treatment
- Trypanosoma species research and implications
- Herpesvirus Infections and Treatments
- Animal Disease Management and Epidemiology
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Research on Leishmaniasis Studies
- HIV/AIDS drug development and treatment
- Drug Transport and Resistance Mechanisms
- Genomic variations and chromosomal abnormalities
- Biochemical and Molecular Research
- Plant Virus Research Studies
- Peroxisome Proliferator-Activated Receptors
- Synthesis and Biological Evaluation
- Pleural and Pulmonary Diseases
- Vector-Borne Animal Diseases
- Hepatitis Viruses Studies and Epidemiology
- Bacteriophages and microbial interactions
- Phenothiazines and Benzothiazines Synthesis and Activities
Tokyo University of Agriculture and Technology
2015-2024
Gifu University
2014-2015
American Type Culture Collection
2003-2013
National Institutes of Health
2003-2012
Yamaguchi University
1997-2010
The University of Tokyo
1989-2008
National Institute of Allergy and Infectious Diseases
2003-2008
Vector Oncology (United States)
2003-2008
Pennsylvania State University
2007
National Sanyo Hospital
2007
The emergence of virulent Plasmodium falciparum in Africa within the past 6000 years as a result cascade changes human behavior and mosquito transmission has recently been hypothesized. Here, we provide genetic evidence for sudden increase African malaria parasite population about 10,000 ago, followed by migration to other regions on basis variation 100 worldwide mitochondrial DNA sequences. However, both world some regional populations appear be older (50,000 100,000 old), suggesting an...
Mutations and/or overexpression of various transporters are known to confer drug resistance in a variety organisms. In the malaria parasite Plasmodium falciparum, homologue P-glycoprotein, PfMDR1, has been implicated responses chloroquine (CQ), quinine (QN) and other drugs, putative transporter, PfCRT, was recently demonstrated be key molecule CQ resistance. However, unknown molecules probably involved, as different clones carrying same pfcrt pfmdr1 alleles show wide range quantitative QN....
Malaria transmission requires the production of male and female gametocytes in human host followed by fertilization sporogonic development mosquito midgut. Although essential for spread malaria through population, little is known about initiation gametocytogenesis vitro or vivo. Using a gametocyte-defective parasite line genetic complementation, we show that Plasmodium falciparum gametocyte 1 gene (Pfgdv1), encoding peri-nuclear protein, critical early sexual differentiation. Transcriptional...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXT6-(1H-Imidazol-1-yl)-7-nitro-2,3(1H,4H)-quinoxalinedione hydrochloride (YM90K) and related compounds: structure-activity relationships for the AMPA-type non-NMDA receptorJ. Ohmori, S. Sakamoto, H. Kubota, M. Shimizu-Sasamata, Okada, Kawasaki, K. Hidaka, J. Togami, T. Furuya, MuraseCite this: Med. Chem. 1994, 37, 4, 467–475Publication Date (Print):February 1, 1994Publication History Published online1 May 2002Published inissue 1 February...
ABSTRACT Histone acetylation, regulated by the opposing actions of histone acetyltransferases (HATs) and deacetylases, is an important epigenetic mechanism in eukaryotic transcription. Although acetyltransferase (PfGCN5) has been shown to preferentially acetylate H3 at K9 K14 Plasmodium falciparum , scale acetylation parasite genome its role transcriptional activation are essentially unknown. Using chromatin immunoprecipitation (ChIP) DNA microarray, we mapped global distribution PfGCN5,...
To better understand the role of histone lysine acetylation in transcription Plasmodium falciparum, we sought to attenuate acetyltransferase (HAT) activity using anacardic acid (AA). We showed that AA reversibly and noncompetitively inhibited HAT recombinant PfGCN5. a lesser extent, PfGCN5 parasite nuclear extracts but did not affect deacetylase activity. blocked growth both chloroquine-sensitive -resistant strains, with 50% inhibitory concentration approximately 30 microM. Treatment...
Trypanosomatids, the etiologic agents of sleeping sickness, leishmaniasis, and Chagas' disease, compartmentalize glycolysis within glycosomes, metabolic organelles related to peroxisomes. Here, we identify a trypanosome homologue PEX14, one components peroxisomal protein import docking complex. We have used double-stranded RNA interference target PEX14 transcript for degradation. Glycosomal matrix was compromised, both glycolytic bloodstream stage parasites mitochondrially respiring...
The study of genetic variation in malaria parasites has practical significance for developing strategies to control the disease. Vaccines based on highly polymorphic antigens may be confounded by allelic restriction host immune response. In response drug pressure, a plastic genome generate resistant mutants more easily than monomorphic one. Additionally, distribution genomic polymorphisms provide information leading identification genes associated with traits such as parasite development and...
Objectives. We monitored the mRNA expression profiles of peripheral blood cells during treatment with a TNF-α inhibitor, infliximab, in patients RA. Using DNA microarray analysis, we demonstrated unique set genes, distinct baseline and post-treatment changes between responders non-responders to infliximab treatment. Methods. customized low-density cDNA 747 genes reliable data collection system, whole from 18 RA before after infusion for up 22 weeks. The clinical response was determined using...
The phosphoinositide (PI)-specific phospholipase C gene (TcPI-PLC) of the protozoan parasiteTrypanosoma cruzi was cloned, sequenced, expressed inEscherichia coli, and protein product shown to have enzymatic characteristics similar those mammalian δ-type PI-PLCs. TcPI-PLC is at high levels in epimastigote amastigote stages parasite, its expression induced during differentiation trypomastigotes into amastigotes, where associates with plasma membrane increases catalytic activity. In contrast...
The termini of viral genomic RNA and its complementary strand are important in the initiation replication, which probably involves both cellular proteins. To detect possible proteins involved replication mouse hepatitis virus RNA, we performed RNA-protein binding studies with RNAs representing 5' 3' ends end negative-strand RNA. Gel-retardation assays showed that 5'-end-positive- 3'-end-negative-strand formed an complex from uninfected cells. UV cross-linking experiments further identified a...
A male gametocyte defect in the Plasmodium falciparum Dd2 parasite was previously discovered through observation that all progeny clones a × HB3 genetic cross were result of fertilization events between female and gametes. determinant linked to subsequently mapped an 800-kb segment on chromosome 12. Here, we report further mapping 82-kb region identification candidate gene, P. development gene 1 ( pfmdv-1 ), is expressed at lower level compared with wild-type normal gametocyte-producing...
We constructed 16 deletion mutants from an infectious molecular clone of feline immunodeficiency virus (FIV) and a reporter plasmid carrying the bacterial chloramphenicol acetyltransferase (CAT) gene to identify rev transactivator activity virus. Cotransfections various bearing portion FIV env in addition CAT revealed that sequence important for augmentation production was located three separate parts genome. This enhancement specific human retrovirus rex products did not activate reporter....
A novel dsRNA virus was identified from the arboreal ant Camponotus yamaokai. The complete nucleotide sequence analysis of revealed that consisted 5704 bp with two ORFs. ORF1 (3084 nt) encoded a putative capsid protein. ORF2 (1977 viral RNA-dependent RNA polymerase (RdRp). could be translated as fusion product by − 1 frameshift in overlapping ORF1. Phylogenetic analyses based on RdRp C. yamaokai most likely totivirus, but it not closely related to previously known totiviruses arthropods....
Abstract To clarify the genetic pathway(s) involved in development and progression of oral squamous cell carcinoma (OSCC), as well relationship between aberrations biological characteristics OSCC tumours, comparative genomic hybridization was used to analyse alterations both primary OSCCs adjacent dysplastic lesions same biopsy specimens from 35 patients. Gain 8q22–23 most frequent alteration mild dysplasia, considered earliest event process tumourigenesis. The average number DNA sequence...