A5062202170- Neurogenesis and neuroplasticity mechanisms
- Carcinogens and Genotoxicity Assessment
- Genomics, phytochemicals, and oxidative stress
- Anesthesia and Neurotoxicity Research
- Neuroscience and Neuropharmacology Research
- Effects and risks of endocrine disrupting chemicals
- Cancer-related Molecular Pathways
- Epigenetics and DNA Methylation
- Neuroinflammation and Neurodegeneration Mechanisms
- Glutathione Transferases and Polymorphisms
- Birth, Development, and Health
- Pharmacogenetics and Drug Metabolism
- Cancer, Hypoxia, and Metabolism
- Potato Plant Research
- Liver physiology and pathology
- Veterinary Oncology Research
- DNA Repair Mechanisms
- Liver Disease Diagnosis and Treatment
- Toxic Organic Pollutants Impact
- Drug Transport and Resistance Mechanisms
- Trace Elements in Health
- Heavy Metal Exposure and Toxicity
- Mycotoxins in Agriculture and Food
- Estrogen and related hormone effects
- Cancer Cells and Metastasis
Tokyo University of Agriculture and Technology
2016-2025
Life Science Institute
2017-2025
Azabu University
2016
Gifu University
2004-2014
Suzuki (Japan)
2014
National Institute of Health Sciences
2003-2012
Division of Chemistry
2009
National Institute of Health Sciences
2001-2008
MRC Laboratory of Molecular Biology
2000
National Institutes of Health
1994-1998
Harmonization of diagnostic nomenclature used in the pathology analysis tissues from rodent toxicity studies will enhance comparability and consistency data sets different laboratories worldwide. The INHAND Project ( International Nomenclature Diagnostic Criteria for Lesions Rats Mice) is a joint initiative four major societies toxicologic to develop globally recognized proliferative nonproliferative lesions rodents. This article recommends standardized terms classifying changes observed...
Indocyanine green (ICG) is a fluorescent agent approved for clinical applications by the Food and Drug Administration European Medicines Agency. This study examined mechanism of tumor imaging using intravenously administered ICG. The in vivo kinetics ICG were determined xenografts microscopic approaches that enabled both spatio-temporal high-magnification analyses. ICG-based was at cellular level six phenotypically different human colon cancer cell lines exhibiting grades epithelioid...
Abstract In human and dogs, bladder cancer ( BC ) is the most common neoplasm affecting urinary tract. Dog resembles muscle‐invasive in histopathological characteristics gene expression profiles, could be an important research model for this disease. Cancer patient‐derived organoid culture can recapitulate organ structures maintains profiles of original tumor tissues. a previous study, we generated dog prostate organoids using urine samples, however had never been produced. Therefore aimed...
Bat is considered a natural reservoir of various important pathogens, including severe acute respiratory syndrome coronavirus (SARS-CoV), SARS-CoV-2, Ebola virus, and Nipah virus. To study these viruses' pathogenicity proliferation efficacy viral tolerance mechanisms in bats, bat-derived cell lines, primary cultured cells are used. However, do not adequately reflect the exact biology establishing new bat-related research models necessary. Organoid culture can recapitulate organ structure,...
Dilated cardiomyopathy is characterized by ventricular dilation with systolic dysfunction of cardiac muscle. Recent genetic studies have revealed that mutations in genes for cytoskeleton proteins distributed the Z-disc and/or intercalated discs muscle are major predictors cardiomyopathy. However, as these can account only a part patient population, there should be another disease-causing gene(s) Cypher/ZASP appears to an ideal candidate causative gene, because encodes associated protein, and...
Bladder cancer (BC), a main neoplasm of urinary tract, is usually inoperable and unresponsive to chemotherapy. As novel experimental model for muscle-invasive BC, we previously established culture method dog BC organoids. In the present study, detailed in vitro vivo anti‐tumor effects trametinib were investigated by using this model. each organoid strain, epidermal growth factor receptor (EGFR)/ERK signaling was upregulated compared with normal bladder cells. Trametinib even at low...
Abstract Three-dimensional (3D) organoid culture holds great promises in cancer precision medicine. However, Matrigel and stem cell-stimulating supplements are necessary for culturing 3D cells. It costs a lot of money consumes more time effort compared with 2D cultured Therefore, the establishment cheaper Matrigel-free that can maintain characteristics part organoids is demanded. In previous study, we established dog bladder (BC) system by using their urine samples. Here, successfully...
Despite its adverse effects, chemotherapy is generally used for the treatment of colorectal cancer (CRC). Development supplement preparations targeting stem cells (CSCs) that cause distant metastasis and drug resistance required. Although curcumin known to have anti-tumor, hepatoprotective, hypoglycemic-like actions, low water solubility, oral absorption, bioavailability impede therapeutic uses. Patient-derived organoid cultures can recapitulate heterogeneity, epithelial structures,...
We have shown that maternal manganese (Mn) exposure caused sustained disruption of hippocampal neurogenesis mouse offspring. To clarify the effects Mn on epigenetic gene regulation contributing to neurogenesis, we treated pregnant ICR mice with MnCl2 in diet from gestational day 10 through 21 after delivery weaning and searched epigenetically downregulated genes by global promoter methylation analysis dentate gyrus male offspring postnatal (PND) PND 77. By CpG microarray following 800-ppm...
Indole-3-carbinol (I3C) has a liver tumor promoting activity in rats, and is also known as cytochrome p450 1A (CYP1A) inducer. The generation of reactive oxygen species (ROS) resulting from CYP1A induction due to I3C, probably involved the promotion. To clarify whether ROS contributes I3C's hepatocellular altered foci, partially hepatectomized rats were fed diet containing 0.5% I3C for 8 weeks with or without 0.3% N-acetyl-L-cysteine (NAC), an antioxidant, their drinking water after...
For molecular analysis in anatomically-specific brain regions for rodent studies, it is necessary to establish a fast and accurate procedure tissue sampling achieve high integrity expression fidelity of extracted molecules. The present study was performed examine suitability whole fixation with methacarn subsequent using punch-biopsy devices gene rats. After fixation, each specific region, i.e., hippocampal dentate gyrus, corpus callosum, cingulate cortex or cerebellar vermis collected, the...
Various pathogens, such as Ebola virus, Marburg Nipah Hendra Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV), Middle East (MERS-CoV), and SARS-CoV-2, are threatening human health worldwide. The natural hosts of these pathogens thought to be bats. rousette bat, a megabat, is reservoir filoviruses, including viruses. Additionally, the bat showed transient infection in experimental inoculation SARS-CoV-2. In current study, we established characterized intestinal organoids from...