A5079840751- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Salivary Gland Disorders and Functions
- Oral microbiology and periodontitis research
- HIV Research and Treatment
- Mosquito-borne diseases and control
- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- Virology and Viral Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Viral Infections and Vectors
- Parvovirus B19 Infection Studies
- Helicobacter pylori-related gastroenterology studies
- Head and Neck Cancer Studies
- Immune Cell Function and Interaction
- Gastric Cancer Management and Outcomes
- Microbial infections and disease research
- interferon and immune responses
- Oral health in cancer treatment
- CRISPR and Genetic Engineering
Osaka University of Pharmaceutical Sciences
2021-2025
Osaka Medical and Pharmaceutical University
2019-2022
Tokyo University of Agriculture and Technology
2018-2019
Kyoto University
2008-2019
Obihiro University of Agriculture and Veterinary Medicine
2008-2019
Kumamoto University
2015
Japan Society for the Promotion of Science
2013
Pathogen mutations present an inevitable and challenging problem for therapeutics the development of mutation-tolerant anti-infective drugs to strengthen global health combat evolving pathogens is urgently needed. While spike proteins on viral surfaces are attractive targets preventing entry, they mutate frequently, making it difficult develop effective therapeutics. Here, we used a structure-guided strategy engineer inhibitor peptide against SARS-CoV-2 spike, called CeSPIACE, with potent...
The raccoon dog (Nyctereutes procyonoides), classified in the order Carnivora within family Canidae, is native to East Asia and widely distributed throughout Japan due its adaptability various environments. Despite close relationship between dogs other animals, viruses infecting have not been thoroughly investigated Japan. In this study, we performed metatranscriptomic analyses using fecal samples collected from latrines of wild two locations on mainland Nearly complete viral genomes were...
Feline morbillivirus (FmoPV) is an emerging virus in domestic cats and considered to be associated with tubulointerstitial nephritis. Although FmoPV was first described China 2012, there has been no report of the isolation this other countries. In report, we describe characterization from Japan. By using reverse transcription (RT)-PCR, found that three 13 urine samples brought veterinary hospitals were positive for FmoPV. strains SS1 SS3 isolated RT-PCR-positive samples. Crandell-Rees feline...
Improved RNA virus understanding is critical to studying animal and plant health, environmental processes. However, the continuous rapid evolution makes their identification characterization challenging. While recent sequence-based advances have led extensive discovery, there growing variation in how viruses are identified, analyzed, characterized, reported. To this end, an RdRp Summit was organized a hybrid meeting took place Valencia, Spain May 2023 convene leading experts with emphasis on...
Oral bacteria have been reported to be associated with the pathogenesis of atherosclerosis; however, relationship between oral microbiota and atherosclerosis remains unclear. The present study aimed investigate whether or not salivary patients atherosclerotic cardiovascular disease (ACVD) differs from that subjects without ACVD, characterize ACVD.
RNA-dependent RNA polymerase (RdRp) is a marker gene for viruses; thus, it widely used to identify viruses from metatranscriptome data. However, because of the high diversity RdRp domains, remains difficult using sequences. To overcome this problem, we created NeoRdRp database containing 1,182 hidden Markov model (HMM) profiles utilizing 12,502 domain Since development database, more have been discovered, mainly through sequencing analyses. comprehensively and specifically, updated by...
To study the genetic diversity of enterovirus G (EV-G) among Japanese pigs, metagenomics sequencing was performed on fecal samples from pigs with or without diarrhea, collected between 2014 and 2016. Fifty-nine EV-G sequences, which were >5,000 nucleotides long, obtained. By complete VP1 sequence analysis, isolates classified into G1 (17 strains), G2 (four G3 (22 G4 (two G6 G9 (six G10 (five a new genotype (one strain). Remarkably, 16 one strain identified in diarrheic (23.5%; four strains)...
Uric acid exerts an important antioxidant effect against external oxidative stress under physiological conditions. However, uric itself can increase via reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase activation in adipocytes and vascular cells. transporter 1 is involved the generation of this stress. Furthermore, locally activates renin–angiotensin system, thus producing angiotensin II subsequently increasing intracellular Benzbromarone has been reported to suppress...
Chikungunya virus (CHIKV) induces a massive cytopathic effect (CPE) on various cell types. Therefore, the plaque assay, CPE-based titration method, remains gold standard for quantifying infectious units of CHIKV. However, manual counting is often labor-intensive task, especially in experiments involving multiple samples. In this study, we developed plaQuest, stand-alone plaque-counting software running Windows operating system, rapid and reliable quantification CHIKV plaques 24-well plate....
RNA viruses are distributed throughout various environments, and most have recently been identified by metatranscriptome sequencing. However, due to the high nucleotide diversity of viruses, it is still challenging identify novel from data. To overcome this issue, we created a dataset RNA-dependent polymerase (RdRp) domains that essential for all belonging Orthornavirae. Genes with RdRp were clustered based on amino acid sequence similarities. A multiple alignment was generated each cluster,...
Rotavirus C (RVC) is a major cause of diarrhoea in swine, cattle, and humans worldwide. RVC exhibits sequence diversity all 11 genes, especially VP4 VP7, segment-based genotyping has been performed similar to rotavirus A. To date, recombination events have reported A B. However, there are no reports describing gene RVC, except for NSP3 between H. In this study, nine porcine strains identified Japanese pigs were completely sequenced analysed together with sequences from the GenBank database....
Cats have an infectious endogenous retrovirus, named RD114 virus, and there is a possibility that virus has contaminated live attenuated vaccines, for which feline cells are used as substrate. To monitor in vaccines cats, we developed LacZ marker rescue assay to detect virus. Among four human cell lines examined, TE671 (human rhabdomyosarcoma) were most susceptible supported replication efficiently. Infection was enhanced approximately 5 times by the addition of polybrene at concentrations 2...
SARS-CoV-2 is the cause of COVID-19. The three-dimensional morphology viral particles existing and multiplying in infected cells has not been established by electron tomography, which different from cryo-electron tomography using frozen samples. In this study, we establish morphological structure reconstruction images obtained transmission microscopy biological samples embedded epoxy resin. characteristic roots spike structures were found to be arranged at surface a virion covered with an...
Feline morbillivirus (FmoPV) is an emerging virus that was recently discovered in domestic cats with chronic nephritis. Despite the potential role of FmoPV nephritis, little known about its biological characteristics. In this study, we established a quantitative assay by using indirect immunofluorescence technique. Viral titers were determined one week. Treatment polybrene® or trypsin which previously used isolation did not augment titers. notably stable at 4°C, retaining high for least 12...
Feline morbillivirus (FmoPV) is an emerging virus in cats, which associated with tubulointerstitial nephritis. To study the vitro host range of FmoPV, we inoculated FmoPV strain SS1 to 32 cell lines originated from 13 species and cultured for 2 weeks, followed by RNA extraction reverse-transcription-polymerase chain reaction detection. As a result, only derived cats African green monkeys were susceptible FmoPV. infects diverse feline lines: epithelial, fibroblastic, lymphoid glial cells....
ABSTRACT In 2001-2002, six of seven Japanese macaques ( Macaca fuscata ) died after developing hemorrhagic syndrome at the Kyoto University Primate Research Institute (KUPRI). While cause death was unknown time, we detected simian retrovirus 4 (SRV-4) in samples obtained from a similar outbreak 2008-2011, during which 42 43 exhibiting syndrome. this study, isolated SRV-4 strain PRI-172 macaque showing severe thrombocytopenia. When inoculated into four macaques, isolate induced...
Oral microbiota may be associated with serious local or systemic medical conditions resulting from chemotherapy. This study was conducted to evaluate the changes in oral following initiation of chemotherapy patients hematopoietic malignancies and identify characteristics mucositis. samples were collected 57 at 2 time points: before start 8 20 days after chemotherapy, when chemotherapy-induced mucositis often occurs, 16S rRNA metagenomic analyses performed. Comparative linear discriminant...