A5066269606- Sphingolipid Metabolism and Signaling
- Slime Mold and Myxomycetes Research
- Lipid Membrane Structure and Behavior
- Chemical synthesis and alkaloids
- Biocrusts and Microbial Ecology
- Glycosylation and Glycoproteins Research
- Lysosomal Storage Disorders Research
- Endoplasmic Reticulum Stress and Disease
- Protein Kinase Regulation and GTPase Signaling
- Peroxisome Proliferator-Activated Receptors
- Cellular transport and secretion
- Lipid metabolism and biosynthesis
- RNA Interference and Gene Delivery
- Diatoms and Algae Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Telomeres, Telomerase, and Senescence
- Drug Transport and Resistance Mechanisms
- Pharmacological Effects of Natural Compounds
- DNA Repair Mechanisms
- Fungal Biology and Applications
- Cell Adhesion Molecules Research
- Cellular Mechanics and Interactions
- Protist diversity and phylogeny
- Heat shock proteins research
- Cell death mechanisms and regulation
Ochanomizu University
2012-2023
Tokyo University of Science
2004-2011
University of Tennessee Health Science Center
1998-2010
Otsuka (Japan)
2004-2009
Richard Wolf (Germany)
2006
Brocade (United States)
2006
University of Tennessee at Knoxville
1996
The University of Tokyo
1976-1992
Nagoya University
1992
Tokyo Metropolitan Institute of Gerontology
1992
Lysophosphatidic acid (LPA), together with sphingosine 1-phosphate, is a bioactive lipid mediator that acts on G-protein-coupled receptors to evoke multiple cellular responses, including Ca<sup>2+</sup> mobilization, modulation of adenylyl cyclase, and mitogen-activated protein (MAP) kinase activation. In this study, we isolated human cDNA encoding novel receptor, designated EDG7, characterized it as receptor for LPA. The amino sequence the EDG7 53.7 48.8% identical those functional LPA EDG2...
Autotaxin (ATX, nucleotide pyrophosphate/phosphodiesterase-2) is an autocrine motility factor initially characterized from A2058 melanoma cell-conditioned medium. ATX known to contribute cancer cell survival, growth, and invasion. Recently was shown be responsible for the lysophospholipase D activity that generates lysophosphatidic acid (LPA). Production of LPA sufficient explain effects on tumor cells. Cyclic phosphatidic (cPA) a naturally occurring analog in which sn-2 hydroxy group forms...
Ro09-0198 (Ro) is a tetracyclic peptide antibiotic that binds specifically to phosphatidylethanolamine (PE) and causes cytolysis. To investigate the molecular basis of transbilayer movement PE in biological membranes, we have isolated series budding yeast mutants are hypersensitive Ro peptide. One most sensitive mutants, designated <i>ros3</i>(Ro-sensitive 3), showed no significant change cellular phospholipid composition or sensitivity amphotericin B, sterol-binding polyene macrolide...
Lysophosphatidic acid (LPA), plasmalogen-glycerophosphate (alkenyl-GP) and, cyclic-phosphatidic (cyclic-PA) are naturally occurring phospholipid growth factors (PLGFs). PLGFs elicit diverse biological effects via the activation of G protein-coupled receptors in a variety cell types. In NIH3T3 fibroblasts, LPA and alkenyl-GP both induced proliferation, whereas cyclic-PA was antiproliferative. decreased cAMP pertussis toxin-sensitive manner, caused to increase. stimulated activity...
A humanized anti‐HER2 monoclonal antibody pertuzumab (Omnitarg, 2C4), binding to a different HER2 epitope than trastuzumab, is known as an inhibitor of heterodimerization the HER receptors. Potent antitumor activity against HER2‐expressing breast and prostate cancer cell lines has been clarified, but this potential not clear lung cancers. The authors investigated in vitro anti‐tumor eight non‐small cells expressing various members 11_18 line expressed large amount HER3, its growth was...
Cholesteryl glucoside (β-ChlGlc), a monoglucosylated derivative of cholesterol, is involved in the regulation heat shock responses. β-ChlGlc, which rapidly induced response to shock, activates transcription factor 1 (HSF1) leading expression protein 70 (HSP70) human fibroblasts. Identification and biochemical characterization enzyme responsible for β-ChlGlc formation important complete understanding molecular mechanisms HSP70-induction following shock. Recently, we demonstrated that...
A specific inhibitor of DNA polymerase alpha was isolated from the lipid fraction prepared myxoamoebae a true slime mold, Physarum polycephalum. The purified substance subjected to structural studies by fast atom bombardment mass spectroscopy, infrared and two-dimensional nuclear magnetic resonance spectroscopy. structure this thereby suggested be novel lysophosphatidic acid (LPA) composed cyclic phosphate cyclopropane-containing hexadecanoic acid. Then we named PHYLPA (Physarum LPA)....
The endothelial differentiation gene family encodes three highly homologous G protein-coupled receptors for lysophosphatidic acid (LPA). Based on baculoviral overexpression studies, differences have been proposed in the structure-activity relationship (SAR) of these receptors. We compared SAR individual either overexpressed transiently at high or lower levels following stable transfection LPA-nonresponsive RH7777 cells. transfected cells was markedly different from that described insect...
Multiple sclerosis is a neuroinflammatory demyelinating and neurodegenerative disease of the central nervous system characterized by recurrent progressive demyelination/remyelination cycles, neuroinflammation, oligodendrocyte loss, demyelination, axonal degeneration. Cyclic phosphatidic acid (cPA) natural phospholipid mediator with unique cyclic phosphate ring structure at sn-2 sn-3 positions glycerol backbone. We reported earlier that cPA elicits neurotrophin-like action protects...
Fetal calf serum (FCS) and 1-oleoyl lysophosphatidic acid (LPA) were previously found to be potent inducers of invasion (transcellular migration) in an vitro system. A novel LPA, composed cyclic phosphate cyclopropane-containing hexadecanoic (PHYLPA), first isolated from myxoamoebae Physarum polycephalum, its synthetic derivatives (cLPA) tested for their ability inhibit tumor cell metastasis. Amoung these, Pal-cLPA, which has a palmitoyl moiety, was most inhibiting invasion, with 93.8%...
Cofilin is a low-molecular weight actin-modulating protein, which binds to, severs, and depolymerizes actin filaments in vitro. Aip1, an actin-interacting was recently identified as product of gene on multicopy plasmid suppresses the temperature-sensitive phenotype cofilin mutant Saccharomyces cerevisiae. Actin cytoskeleton plays essential role resistance to hyperosmotic stress Dictyostelium discoideum. The roles Aip1 this are not known.In response stress, D. discoideum cells round up. This...
We previously reported that steryl glucoside (SG) is rapidly induced in cells from molds to humans by exposure environmental stress (Murakami-Murofushi et al. (1997) J. Biol. Chem., 272, 486-489, Kunimoto (2000) Cell Stress & Chaperones, 5, 3-7), and mold SG production followed activation of a certain protein kinase induction heat shock proteins (HSP) (Maruya Struct. Funct., 21, 533-538). To determine the biological significance responsive signal transduction, we added culture human...
Cyclic phosphatidic acid (cPA; 1-acyl-sn-glycerol-2,3-cyclic phosphate) is an analog of the growth factor-like phospholipid mediator lysophosphatidic (LPA). As brain tissue richest source cPA we tested its effects on hippocampal neurons from day 16/17 embryonic rat cultured in a serum-free medium. Nanomolar concentrations elicited neurotrophic effect and promoted neurite outgrowth that exceeded 50 ng/mL nerve factor (NGF). Pertussis toxin, LPA1/LPA3 receptor-selective antagonist...
In Xenopus laevis oocytes, both lysophosphatidic acid (LPA) and a cyclic phosphate-containing analogue 1-acyl-sn-glycero-2,3-cyclic phosphate (cLPA) isolated from Physarum polycephalum activated oscillatory Cl- currents. cLPA elicited currents only when applied extracellularly and, similarly to LPA, evoked homologous desensitization. oocytes previously desensitized b y LPA failed elicit current, indicating that completely the receptors. contrast, were by cLPA, still large The lack of...