A5034012372- Mosquito-borne diseases and control
- Malaria Research and Control
- Vector-borne infectious diseases
- vaccines and immunoinformatics approaches
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Poxvirus research and outbreaks
- Leptospirosis research and findings
- Pneumonia and Respiratory Infections
- Bacteriophages and microbial interactions
- HIV Research and Treatment
- Herpesvirus Infections and Treatments
- Bacillus and Francisella bacterial research
- Respiratory viral infections research
- Brucella: diagnosis, epidemiology, treatment
- Complement system in diseases
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- COVID-19 Clinical Research Studies
- Salmonella and Campylobacter epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Invertebrate Immune Response Mechanisms
- Biosensors and Analytical Detection
- Syphilis Diagnosis and Treatment
Antigen Discovery (United States)
2014-2025
University of California, Irvine
2009-2020
Irvine Valley College
2019
University of Maryland, Baltimore
2014
Howard Hughes Medical Institute
2014
National Institutes of Health
2008-2014
Medicina
2013
National Institute of Allergy and Infectious Diseases
2008
Texas A&M Health Science Center
2008
Washington State University
2008
Understanding the way in which immune system responds to infection is central development of vaccines and many diagnostics. To provide insight into this area, we fabricated a protein microarray containing 1,205 Burkholderia pseudomallei proteins, probed it with 88 melioidosis patient sera, identified 170 reactive antigens. This subset antigens was printed on smaller array collection 747 individual sera derived from 10 groups including patients Northeast Thailand Singapore, different...
Abstract The eradication of smallpox by vaccination with vaccinia virus was probably one the greatest achievements vaccinology. However, immunological basis this protection is not fully understood. To end, we have used protein microarrays (Western Reserve, WR) proteome to profile antibody reactivities after primary infection or boosting licensed vaccine, Dryvax®, and archival convalescent sera. Some 25 antigens were consistently recognized Dryvax® sera, which half envelope proteins (notably,...
Individuals that are exposed to malaria eventually develop immunity the disease with one possible mechanism being gradual acquisition of antibodies range parasite variant surface antigens in their local area. Major antibody targets include large and highly polymorphic Plasmodium falciparum Erythrocyte Membrane Protein 1 (PfEMP1) family proteins. Here, we use a protein microarray containing 123 recombinant PfEMP1-DBLα domains (VAR) from Papua New Guinea seroprofile 38 nonimmune children (<4...
Infection with Plasmodium can elicit antibodies that inhibit parasite survival in the mosquito, when they are ingested an infectious blood meal. Here, we determine transmission-reducing activity (TRA) of naturally acquired from 648 malaria-exposed individuals using lab-based mosquito-feeding assays. Transmission inhibition is significantly associated antibody responses to Pfs48/45, Pfs230, and 43 novel gametocyte proteins assessed by protein microarray. In field-based assays likelihood rate...
Abstract Complete sterile protection to Plasmodium falciparum ( Pf ) infection mediated by pre-erythrocytic immunity can be experimentally induced under chloroquine prophylaxis, through immunization with sporozoites from infected mosquitoes' bites (CPS protocol). To characterize the profile of CPS antibody (Ab) responses, we developed a proteome microarray containing 809 antigens showing distinct Ab recognition expressed in life-cycle stages. In contrast, plasma naturally exposed semi-immune...
Babesia microti, a tick-transmitted, intraerythrocytic protozoan parasite circulating mainly among small mammals, is the primary cause of human babesiosis. While most cases are transmitted by Ixodes ticks, disease may also be through blood transfusion and perinatally. A comprehensive analysis genome composition, genetic diversity, gene expression profiling seven B. microti isolates revealed that variation in from Northeast United States almost exclusively associated with genes encoding...
Schistosomiasis is a neglected tropical disease that responsible for almost 300,000 deaths annually. Mass drug administration (MDA) used worldwide the control of schistosomiasis, but chemotherapy fails to prevent reinfection with schistosomes, so MDA alone not sufficient eliminate disease, and prophylactic vaccine required. Herein, we take advantage recent advances in systems biology longitudinal studies schistosomiasis endemic areas Brazil pilot an immunomics approach discovery antigens. We...
ABSTRACT Q fever is a widespread zoonosis caused by Coxiella burnetii . Diagnosis of usually based on serological testing patient serum. The diagnostic antigen test kits formalin-fixed phase I and II organisms the Nine Mile reference strain. Deficiencies this include (i) potential for cross-reactivity with other pathogens; (ii) an inability to distinguish between C. strains; (iii) need propagate purify , difficult potentially hazardous process. Consequently, there sensitive specific...
Antigen profiling using comprehensive protein microarrays is a powerful tool for characterizing the humoral immune response to infectious pathogens. Coxiella burnetii CDC category B bioterrorist agent with worldwide distribution. In order assess antibody repertoire of acute and chronic Q fever patients we have constructed microarray containing 93% proteome burnetii, causative fever. Here report profile IgG IgM seroreactivity in 25 longitudinal samples. We found that both early late time...
Abstract Comprehensive evaluation of the humoral immune response to Coxiella burnetii may identify highly needed diagnostic antigens and potential subunit vaccine candidates. Here we report construction a protein microarray containing 1901 C. ORFs (84% entire proteome). This array was probed with Q‐fever patient sera naïve controls in order discover ‐specific seroreactive antigens. Among 21 identified, 13 were significantly more reactive cases than controls. The remaining eight...
Routine serodiagnosis of herpes simplex virus (HSV) infections is currently performed using recombinant glycoprotein G (gG) antigens from 1 (HSV-1) and HSV-2. This a single-antigen test has only one diagnostic application. Relatively little known about HSV antigenicity at the proteome-wide level, full potential mining antibody repertoire to identify with other useful properties candidate vaccine yet be realized. To this end we produced HSV-1 -2 proteome microarrays in Escherichia coli probed...
Leptospirosis is a widespread zoonotic disease worldwide. The lack of an adequate laboratory test major barrier for diagnosis, especially during the early stages illness, when antibiotic therapy most effective. Therefore, there critical need efficient diagnostic this life threatening disease.In order to identify new targets that could be used as makers leptopirosis, we constructed protein microarray chip comprising 61% Leptospira interrogans proteome and investigated IgG response from 274...
The rapid worldwide spread of SARS-CoV-2 has accelerated research and development for controlling the COVID-19 pandemic. A multi-coronavirus protein microarray was created containing full-length proteins, overlapping fragments various lengths, peptide libraries from four other human coronaviruses. Sera confirmed patients as well unexposed individuals were applied to multicoronavirus arrays identify specific antibody reactivity. High-level IgG, IgM, IgA reactivity structural proteins S, M, N...
BACKGROUND. The use of high-throughput technologies has enabled rapid advancement in the knowledge host immune responses to pathogens. Our objective was compare repertoire, protection, and maternal factors associated with human milk antibodies infectious pathogens different economic geographic locations.
ABSTRACT Inactivated, whole-cell pertussis (wP) vaccines remain at the frontline in global fight against resurgence of whooping cough, especially low- and middle-income countries. However, reliance on intracerebral mouse potency test (ic-MPT or Kendrick assay) as standard batch release assay is extremely burdensome for commercial wP vaccine production. The ic-MPT technically challenging, labor intensive, incongruous with modern animal welfare guidelines. Replacing a Bordetella enzyme-linked...
ABSTRACT Shigella is a leading cause of diarrheal morbidity and mortality in young children from low- middle-income countries. Here, we aimed to verify the ability generalized modules for membrane antigens (GMMA)-based sonnei candidate vaccine 1790GAHB elicit an anti-protein antibody response. Serum samples previous clinical trials adults (a dose-escalation study its extension France, efficacy after human challenge United States, Kenya) were investigated using pan-proteome microarrays...
Variant surface antigens (VSAs) play a critical role in severe malaria pathogenesis. Defining gaps, or "lacunae", immunity to these Plasmodium falciparum children with would improve our understanding of vulnerability and how protective develops. Using protein microarray 179 antigen variants from three VSA families as well more than 300 other blood stage P. antigens, reactivity was measured sera Malian cerebral malarial anaemia age-matched controls. Sera recognized fewer extracellular PfEMP1...
Leptospirosis is an important zoonotic disease worldwide. Humans usually present a mild non-specific febrile illness, but proportion of them develop more severe outcomes, such as multi-organ failure, lung hemorrhage and death. Such complications are thought to depend on several factors, including the host immunity. Protective immunity associated with humoral immune response, little known about response mounted during naturally-acquired Leptospira infection.Here, we used protein microarray...
Current diagnostic tests for typhoid fever, the disease caused by Salmonella Typhi, are poor. We aimed to identify serodiagnostic signatures of fever assessing microarray signals 4,445 S. Typhi antigens in sera from 41 participants challenged with oral Typhi. found broad, heterogeneous antibody responses increasing IgM/IgA at diagnosis. In down-selected 250-antigen arrays we validated a second challenge cohort (n=30), and selected using machine learning multivariable modelling. four models...
BACKGROUND Babesia microti is a protozoan parasite responsible for the majority of reported cases human babesiosis and major risk to blood supply. Laboratory screening donors may help prevent transfusion‐transmitted but there no Food Drug Administration–approved method yet available. Development sensitive, specific, highly automated B. antibody assay diagnosis acute could have an important impact on decreasing health burden infection. STUDY DESIGN AND METHODS Herein, we take advantage recent...
Abstract Vaccines based on Plasmodium falciparum apical membrane antigen 1 (AMA1) have failed due to extensive polymorphism in AMA1. To assess the strain-specificity of antibody responses malaria infection and AMA1 vaccination, we designed protein peptide microarrays representing hundreds unique variants. Following clinical episodes, children had short-lived, sequence-independent increases average whole-protein seroreactivity, as well strain-specific peptides diverse epitopes. Vaccination...
There is no vaccine to protect from cryptosporidiosis, a leading cause of diarrhea in infants low and middle income countries. Here we comprehensively identified parasite antigens associated with protection reinfection. A Cryptosporidium protein microarray was constructed by vitro transcription translation 1761 C. parvum, hominis or meleagridis antigens, including proteins signal peptide and/or transmembrane domain. Plasma IgG IgA Bangladeshi children longitudinally followed for...
Given the resurgence of syphilis, research endeavors to improve current assays for serological diagnosis and management this disease are a priority. A proteome-scale platform high-throughput profiling humoral response
Brucellosis is a widespread zoonotic disease that also potential agent of bioterrorism. Current serological assays to diagnose human brucellosis in clinical settings are based on detection agglutinating anti-LPS antibodies. To better understand the universe antibody responses develop after B. melitensis infection, protein microarray was fabricated containing 1,406 predicted proteins. The array probed with sera from experimentally infected goats and naturally humans an endemic region Peru....
A complete understanding of the factors that determine selection antigens recognized by humoral immune response following infectious agent challenge is lacking. Here we illustrate a systems biology approach to identify antibody signature associated with Brucella melitensis (Bm) infection in humans and predict proteomic features serodiagnostic antigens. By taking advantage full proteome microarray expressing previously cloned 1406 newly 1640 Bm genes, were able 122 immunodominant 33 The...