A5044988737- Malaria Research and Control
- Vector-borne infectious diseases
- Parasitic Infections and Diagnostics
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Viral Infections and Immunology Research
- Genomics and Phylogenetic Studies
- Insect symbiosis and bacterial influences
- Animal Disease Management and Epidemiology
- Clostridium difficile and Clostridium perfringens research
- RNA and protein synthesis mechanisms
- Parasite Biology and Host Interactions
- Toxoplasma gondii Research Studies
- Bacterial Genetics and Biotechnology
- Genetics, Bioinformatics, and Biomedical Research
- Coccidia and coccidiosis research
- Agricultural pest management studies
- Plant and Fungal Interactions Research
- Trypanosoma species research and implications
- Plant Virus Research Studies
- Biofuel production and bioconversion
- Insect Pest Control Strategies
- Botulinum Toxin and Related Neurological Disorders
- Insect and Pesticide Research
- Tumors and Oncological Cases
Institut de Recherche en Cancérologie de Montpellier
2015-2024
Université de Montpellier
2013-2024
Inserm
2015-2024
National Institute Of Hygiene And Epidemiology
2024
Institut Regional du Cancer de Montpellier
2019-2023
Institut de Radiobiologie Cellulaire et Moléculaire
2020-2021
Institut Montpelliérain Alexander Grothendieck
2013-2019
Laboratoire de Biologie Computationnelle et Quantitative
2019
Centre National de la Recherche Scientifique
1998-2017
Laboratoire d'Informatique, de Robotique et de Microélectronique de Montpellier
2017
Microsporidia are obligate intracellular parasites infesting many animal groups1. Lacking mitochondria and peroxysomes, these unicellular eukaryotes were first considered a deeply branching protist lineage2 that diverged before the endosymbiotic event led to mitochondria. The discovery of gene for mitochondrial-type chaperone3,4,5 combined with molecular phylogenetic data6,7,8,9 later implied microsporidia atypical fungi lost during evolution. Here we report DNA sequences 11 chromosomes...
Degeneration is the process whereby Clostridium acetobutylicum ATCC 824 loses capacity to produce acetone and butanol after repeated vegetative transfers or in continuous culture. Two degenerate mutants (M5 DG1) of C. do not contain four genes (ctfA, ctfB, adc, aad) for formation. Strain contains a 210-kb plasmid (pSOL1) which absent M5 DG1. pSOL1 carries formation genes. A restriction map was constructed by using ApaI, SmaI, SstII, NarI digestions. DG1 could be complemented expressing...
We have sequenced the genome of emerging human pathogen Babesia microti and compared it with that other protozoa. B. has smallest nuclear among all Apicomplexan parasites to date three chromosomes encoding ∼3500 polypeptides, several which are species specific. Genome-wide phylogenetic analyses indicate is significantly distant from Babesidae Theileridae defines a new clade in phylum Apicomplexa. Furthermore, unlike Apicomplexa, its mitochondrial circular. Genome-scale reconstruction...
Summary The location of the cpe gene, encoding enterotoxin responsible for food poisoning in humans, has been studied a series enterotoxigenic Ciostridium perfringens strains by means pulsed field gel electrophoresis genomic DNA. gene was found at same chromosomal locus associated with humans and shown to be linked repetitive sequence, Hin dlll repeat, an open reading frame, ORF3, that may part insertion sequence. In contrast, when originated from domesticated livestock located on large...
Babesiosis is a malaria-like disease in humans and animals that caused by Babesia species, which are tick-transmitted apicomplexan pathogens. duncani causes severe to lethal infection humans, but despite the risk this parasite poses as an emerging pathogen, little known about its biology, metabolic requirements or pathogenesis. Unlike other parasites infect red blood cells, B. can be continuously cultured vitro human erythrocytes mice resulting fulminant babesiosis death. We report...
The main goals of this work were to identify the metabolic pathways bacterial community in a lacustrine ecosystem and establish links between taxonomic composition relative abundances these pathways. For purpose, we analysed 16S rRNA gene library obtained by amplification together with sequence both insert ends on c. 7700 fosmids. Whatever used, Actinobacteria was most abundant group, followed Proteobacteria Bacteroidetes. Specific aquatic clades such as acI acIV (Actinobacteria) or LD12...
Babesia microti, a tick-transmitted, intraerythrocytic protozoan parasite circulating mainly among small mammals, is the primary cause of human babesiosis. While most cases are transmitted by Ixodes ticks, disease may also be through blood transfusion and perinatally. A comprehensive analysis genome composition, genetic diversity, gene expression profiling seven B. microti isolates revealed that variation in from Northeast United States almost exclusively associated with genes encoding...
The study of cellular networks mediated by ligand-receptor interactions has attracted much attention recently owing to single-cell omics. However, rich collections bulk data accompanied with clinical information exists and continue be generated no equivalent in so far. In parallel, spatial transcriptomic (ST) analyses represent a revolutionary tool biology. A large number ST projects rely on multicellular resolution, for instance the Visium™ platform, where several cells are analyzed at each...
de documents scientifiques niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
de niveau recherche, publiés ou non, émanant des établissements d'enseignement et recherche français étrangers, laboratoires publics privés.
Carbon catabolite repression is mediated in Aspergillus nidulans by the negative acting protein CreA. The CreA repressor plays a major role control of expression alc regulon, encoding proteins required for ethanol utilization pathway. It represses directly, at transcriptional level, specific transacting gene alcR, two structural genes alcA and aldA, other all physiological growth conditions. Among seven putative sites identified alcApromoter region, we have determined functional targets AlcR...
The human malaria parasite Plasmodium falciparum is absolutely dependent on the acquisition of host pantothenate for its development within erythrocytes. Although biochemical properties this transport have been characterized, molecular identity parasite-encoded transporter remains unknown. Here we report identification and functional characterization first protozoan transporter, PfPAT, from P. falciparum. We show using cell biological, biochemical, genetic analyses that localized to plasma...
Babesia microti is the primary causative agent of human babesiosis, an emerging pathogen that causes a malaria-like illness with possible fatal outcome in immunocompromised patients. The genome sequence B. R1 strain was reported 2012 and revealed distinct evolutionary path for this relative to other apicomplexa. Lacking from first assembly initial molecular analyses information about terminal ends each chromosome, both exact number chromosomes nuclear organization mitochondrial remained...
Dengue virus remains a major threat in Vietnam, while chikungunya is expected to become one. Surveillance was conducted from 2012 2014 Vietnam assess the presence of dengue and viruses patients hospitalized with acute fever five provinces neighboring Lao PDR Cambodia. extended mosquitoes present vicinity patients' households.A total 558 human serum samples were collected along 1104 adult 12,041 larvae 2250 households. found 17 (3%) 9 (0.8%) mosquitoes. Chikungunya detected 2 (0.18%) no...
BACKGROUND Babesia microti is a protozoan parasite responsible for the majority of reported cases human babesiosis and major risk to blood supply. Laboratory screening donors may help prevent transfusion‐transmitted but there no Food Drug Administration–approved method yet available. Development sensitive, specific, highly automated B. antibody assay diagnosis acute could have an important impact on decreasing health burden infection. STUDY DESIGN AND METHODS Herein, we take advantage recent...
Babesiosis, caused by protozoan parasites of the genus Babesia, is an emerging tick-borne disease significance for both human and animal health. Babesia infect erythrocytes vertebrate hosts where they develop multiply rapidly to cause pathological symptoms associated with disease. The identification new species underscores ongoing risk zoonotic pathogens capable infecting humans, a concern amplified anthropogenic activities environmental changes. One such pathogen, MO1, previously implicated...
A restriction map of the 2.8-Mb genome unicellular eukaryote Encephalitozoon cuniculi (phylum Microspora), a mammal-infecting intracellular parasite, has been constructed using two enzymes with 6 bp recognition sites (BssHII and MluI). The fragments resulting from either single digestions whole molecular karyotype or double 11 individual chromosomes have separated by two-dimensional pulsed field gel electrophoresis (2D-PFGE) procedures. average distance between successive is ~19 kb. terminal...
The apicomplexan intraerythrocytic parasite Babesia microti is an emerging human pathogen and the primary cause of babesiosis, a malaria-like illness endemic in United States. transmitted to humans by tick vector, Ixodes scapularis, transfusion blood from asymptomatic B. microti-infected donors. Whereas nuclear mitochondrial genomes this have been sequenced, assembled annotated, its apicoplast genome remained incomplete, mainly due low representation high A+T content. Here we report complete...
Multiple synergistic combination approaches with cancer drugs are developed to overcome primary resistance immunotherapy; however, the mechanistic rationale combine chemoradiotherapy (CRT) immune checkpoint inhibitors remains elusive. This study described immunological landscape of tumor microenvironment (TME) exposed CRT. Tumor samples from patients rectal (n=43) treated neoadjuvant CRT or radiotherapy were analyzed by nanostring and immunohistochemistry. Studies in mice performed using...
Protozoan parasites are known to attach specific and diverse group of proteins their plasma membrane via a GPI anchor. In malaria parasites, GPI-anchored (GPI-APs) have been shown play an important role in host-pathogen interactions key function host cell invasion immune evasion. Because immunogenic properties, some these considered as vaccine candidates. However, identification all possible GPI-APs encoded by remains challenging due sequence diversity limitations the tools used for...
In 2011-2012, Northern Vietnam suffered its first large scale hand foot and mouth disease (HFMD) epidemic. Two sets of official guidelines were issued during the outbreak to handle HFMD crisis. The city Hai Phong was therefore used as a model analyze impact release. 9621 cases reported in from April 2011 December 2012. Three distinct waves occurred. Enterovirus A71 Coxackievirus A16 successively associated epidemics. periods, before after realease, could be distinguished which characterized...
ABSTRACT Babesia and Plasmodium pathogens, the causative agents of babesiosis malaria, are vector-borne intraerythrocytic protozoan parasites, posing significant threats to both human animal health. The widespread resistance exhibited by these pathogens various classes antiparasitic drugs underscores need for development novel more effective therapeutic strategies. Antifolates have long been recognized as attractive they target folate pathway, which is essential biosynthesis purines...
A simple method for complete genome radiolabelling is described, involving long-wave UV exposure of agarose-embedded chromosomal DNA and [α-32P]dCTP incorporation mediated by the Klenow fragment. Experiments on budding yeast show that labelling procedure can be coupled with two new two-dimensional pulsed field gel electrophoresis (2D-PFGE) protocols analysis: (i) KARD (karyotype restriction display)-PFGE which provides a view fragments resulting from single whole (ii) DDIC (double digestion...
Abstract The metabolic machinery for the biosynthesis of Coenzyme A (CoA) from exogenous pantothenic acid (Vitamin B 5 ) has long been considered as an excellent target development selective antimicrobials. Earlier studies in human malaria parasite Plasmodium falciparum have shown that pantothenate analogs interfere with phosphorylation and block asexual blood stage development. Although two eukaryotic-type putative kinase genes ( PanK1 PanK2 identified all species, their role life cycle...