A5024695151- Malaria Research and Control
- Mosquito-borne diseases and control
- Global Maternal and Child Health
- Computational Drug Discovery Methods
- Parasites and Host Interactions
- Invertebrate Immune Response Mechanisms
- Complement system in diseases
- Child Nutrition and Water Access
- Trypanosoma species research and implications
- Drug Transport and Resistance Mechanisms
- Hemoglobinopathies and Related Disorders
- SARS-CoV-2 and COVID-19 Research
- Drug-Induced Hepatotoxicity and Protection
- Neonatal Health and Biochemistry
- COVID-19 Clinical Research Studies
- Pregnancy and preeclampsia studies
- Plant Pathogenic Bacteria Studies
- Methemoglobinemia and Tumor Lysis Syndrome
- Pharmacological Effects and Toxicity Studies
- Insect Resistance and Genetics
- Psidium guajava Extracts and Applications
- Insect symbiosis and bacterial influences
- Vaccine Coverage and Hesitancy
- SARS-CoV-2 detection and testing
- HIV Research and Treatment
Université des Sciences, des Techniques et des Technologies de Bamako
2016-2025
University of Bamako
2011-2021
National Institute of Malaria Research
2020
Maladies Infectieuses et Vecteurs: Écologie, Génétique, Évolution et Contrôle
2018
Institut de Recherche pour le Développement
2018
Centre National de la Recherche Scientifique
2018
A randomized trial reported by Alassane Dicko and colleagues shows that intermittent preventive treatment for malaria in children who are protected from mosquitoes insecticide-treated bednets provides substantial protection malaria.
Understanding the importance of gametocyte density on human-to-mosquito transmission is immediate relevance to malaria control. Previous work (Churcher et al., 2013) indicated a complex relationship between and mosquito infection. Here we use data from 148 feeding experiments naturally infected carriers show that much simpler depends both female male parasite density. The proportion mosquitoes primarily determined by gametocytes though low densities may be impeded lack parasites. Improved...
Seasonal malaria chemoprevention (SMC) is a new strategy recommended by WHO in areas of highly seasonal transmission March 2012. Although randomized controlled trials (RCTs) have shown SMC to be effective, evidence and experience from routine implementation are limited. A non-randomized pragmatic trial with pre-post design was used, one intervention district (Kita), where four rounds sulfadoxine + amodiaquine (SP AQ) took place August–November 2014, comparison (Bafoulabe). The primary aims...
BackgroundPrimaquine and methylene blue are gametocytocidal compounds that could prevent Plasmodium falciparum transmission to mosquitoes. We aimed assess the efficacy safety of primaquine in preventing human mosquito P among glucose-6-phosphate dehydrogenase (G6PD)-normal, gametocytaemic male participants.MethodsThis was a phase 2, single-blind, randomised controlled trial done at Clinical Research Centre Malaria Training (MRTC) University Bamako (Bamako, Mali). enrolled participants aged...
The transmission of malaria to mosquitoes depends on the presence gametocytes that circulate in peripheral blood infected human hosts. Sensitive estimates densities female (FG) and male (MG) may allow prediction infectivity thus a molecular estimate infectious reservoir for transmission. A novel multiplex qRT-PCR assay with intron-spanning primers was developed parallel quantification FG MG. CCp4 (PF3D7_0903800) transcripts specific PfMGET (PF3D7_1469900) MG were quantified total nucleic...
Single low-dose primaquine (PQ) reduces Plasmodium falciparum infectivity before it impacts gametocyte density. Here, we examined the effect of PQ on sex ratio as a possible explanation for this early sterilizing effect.
Background The R21/Matrix-M malaria vaccine has been shown to provide high protective efficacy against in a phase III trial, and recommended for use by WHO. will soon be deployed at scale sub-Saharan Africa. This study aimed understand the caregiver community acceptability of alongside existing prevention interventions, according communities participants seasonal trial Mali. Methods Qualitative data were collected assess three or control priming injections given first year trial. A total 33...
Abstract Background In polyclonal human malaria infections, the roles of individual clones in human-to-mosquito transmission and their relative transmissibility remain poorly understood. addition, mutations conferring drug resistance can result a advantage or disadvantage. Methods Amplicon sequencing complexity infection markers was used to analyse post-treatment stage-specific parasite dynamics blood infections midguts mosquitoes that became infected after direct membrane feeding assays...
Severe acute malnutrition ( SAM ) has been reported to be associated with increased malaria morbidity in Sub‐Saharan African children and may affect the pharmacology of antimalarial drugs. This population pharmacokinetic (PK)‐pharmacodynamic study included 131 266 non‐ administered artemether‐lumefantrine twice daily for 3 days. Lumefantrine capillary plasma concentrations were adequately described by two transit‐absorption compartments followed distribution compartments. Allometrically...
Pyronaridine-artesunate is the most recently licensed artemisinin-based combination therapy. WHO has recommended that a single low dose of primaquine could be added to therapies reduce
Quantifying gametocyte densities in natural malaria infections is important to estimate transmission potential. Two molecular methods (Pfs25 mRNA quantitative reverse transcriptase PCR (qRT-PCR) and Pfs25 nucleic acid sequence based amplification (QT-NASBA)) are commonly used determine clinical epidemiological studies allow detection at below the microscopic threshold for detection. Here, reproducibility of these measurements association between estimated mosquito infection rates were...
Abstract Gametocyte density and sex ratio can predict the proportion of mosquitoes that will become infected after feeding on blood patients receiving nongametocytocidal drugs. Because primaquine methylene blue sterilize gametocytes before affecting their ratio, mosquito experiments are required to demonstrate early transmission-blocking effects.
Severe acute malnutrition (SAM) affects almost all organs and has been associated with reduced intestinal absorption of medicines. However, very limited information is available on the pharmacokinetic properties antimalarial drugs in this vulnerable population. We assessed artemether-lumefantrine (AL) clinical efficacy children SAM compared to those without. Children under 5 years age uncomplicated P. falciparum malaria were enrolled between November 2013 January 2015 Mali Niger, one third...
Seasonal vaccination with the RTS,S/AS01
Background Intermittent preventive treatment of malaria in children (IPTc) is a highly efficacious method control where transmission seasonal. However, no studies published to date have examined community perceptions IPTc. Methods A qualitative study was undertaken parallel with double-blind, placebo-controlled, randomized trial IPTc conducted Mali and Burkina Faso 2008–2009 assess recommendations for Caregivers health workers (CHWs) were purposively sampled. Seventy-two in-depth individual...
The World Health Organization recommendation on the use of a single low dose primaquine (SLD-PQ) to reduce Plasmodium falciparum malaria transmission requires more safety data. We conducted an open-label, nonrandomized, dose-adjustment trial 3 doses in glucose-6-phosphate dehydrogenase (G6PD)-deficient adult males Mali, followed by assessment G6PD-deficient boys aged 11–17 years and those 5–10 years, including G6PD-normal control groups. primary outcome was greatest within-person percentage...
Emergence of high-grade sulfadoxine-pyrimethamine (SP) resistance in parts Africa has led to growing concerns about the efficacy intermittent preventive treatment malaria during pregnancy (IPTp) with SP. The incremental cost-effectiveness screening and (ISTp) artemether-lumefantrine (AL) as an alternative strategy IPTp-SP was estimated followed by a simulation effects on decreasing due SP resistance. analysis based results from multi-centre, non-inferiority trial conducted West Africa.A...
Background The WHO recommends use of the RTS,S/AS01 E (RTS,S) malaria vaccine for young children living in areas moderate to high Plasmodium falciparum transmission and suggests countries consider seasonal vaccination with highly malaria. Seasonal is uncommon may require adaptations potential cost consequences. This study prospectively estimates delivery Mali Burkina Faso. Methods Three scenarios are costed (1) mass campaign only, (2) routine Expanded Programme on Immunisation (EPI) (3)...
Background: Plasmodium falciparum (Pf) parasitemia during pregnancy causes maternal, fetal, and infant mortality. We conducted two trials in Ouélessébougou, Mali to optimize PfSPZ Vaccine dosage, schedule, antimalarial pre-treatment for future maternal immunizations.Methods: In MLSPZV3 trial (NCT03510481), we randomly assigned healthy 18-35-year-olds 3 doses of 9x105 (versus placebo) at 0,1,4-weeks or 0,8,16-weeks without pre-treatment, with 1-year booster dose. MLSPZV4 (NCT03989102),...
ABSTRACT Background Interrupting human-to-mosquito transmission is important for malaria elimination strategies as it can reduce infection burden in communities and slow the spread of drug resistance. Antimalarial medications differ their efficacy clearing stages Plasmodium falciparum (gametocytes) preventing mosquito infection. Here we present a combined analysis six trials conducted at same study site with highly consistent methodologies that allows direct comparison gametocytocidal...
Clinical studies have shown that adding a single 0.25 mg base/kg dose of primaquine to standard antimalarial regimens rapidly sterilizes Plasmodium falciparum gametocytes. However, the mechanism action and overall impact on malaria transmission is still unknown. Using data from 81 adult Malians with P. gametocytemia who received dihydroartemisinin-piperaquine treatment course were randomized receive either between 0.0625 0.5 or placebo, we characterized pharmacokinetic-pharmacodynamic...
Background: Serological tests are an indispensable tool to understand the epidemiology of SARS-CoV-2 pandemic, particularly in areas where molecular diagnostics limited. Poor assay performance may hinder utility these tests, including high rates false-positivity previously reported sub-Saharan Africa.Methods: From 312 Malian samples collected prior 2020, we measured antibodies commonly tested antigens and four other betacoronaviruses by ELISA, assessed functional cross-reactivity a subset...