Deus S. Ishengoma
A5056509819- Malaria Research and Control
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Parasites and Host Interactions
- Global Maternal and Child Health
- Trypanosoma species research and implications
- Travel-related health issues
- Viral Infections and Outbreaks Research
- HIV/AIDS drug development and treatment
- Genetics, Bioinformatics, and Biomedical Research
- Hemoglobinopathies and Related Disorders
- vaccines and immunoinformatics approaches
- Viral Infections and Vectors
- Research on Leishmaniasis Studies
- Digital Imaging for Blood Diseases
- Drug-Induced Hepatotoxicity and Protection
- Vibrio bacteria research studies
- Invertebrate Immune Response Mechanisms
- Vaccine Coverage and Hesitancy
- Aquaculture disease management and microbiota
- HIV/AIDS Research and Interventions
- HIV/AIDS Impact and Responses
- Complement system in diseases
- Hepatitis C virus research
- CRISPR and Genetic Engineering
Kampala International University
2024-2025
National Institute for Medical Research
2016-2025
Monash University
2020-2024
Harvard University
2020-2024
International University
2024
Centre for Human Genetics
2023
University of Oxford
2023
University of Ghana
2015
University of Copenhagen
2009-2014
Copenhagen University Hospital
2009-2014
Mutations in the Plasmodium falciparum K13-propeller domain have recently been shown to be important determinants of artemisinin resistance Southeast Asia. This study investigated prevalence polymorphisms across sub-Saharan Africa. A total 1212 P. samples collected from 12 countries were sequenced. None mutations previously reported Asia found, but 22 unique detected, which 7 nonsynonymous. Allele frequencies ranged between 1% and 3%. Three observed >1 country, A578S was present parasites 5...
MalariaGEN is a data-sharing network that enables groups around the world to work together on genomic epidemiology of malaria. Here we describe new release curated genome variation data 7,000 Plasmodium falciparum samples from partner studies in 28 malaria-endemic countries. High-quality genotype calls 3 million single nucleotide polymorphisms (SNPs) and short indels were produced using standardised analysis pipeline. Copy number variants associated with drug resistance structural cause...
<ns3:p>We describe the MalariaGEN Pf7 data resource, seventh release of <ns3:italic>Plasmodium falciparum</ns3:italic> genome variation from network. It comprises over 20,000 samples 82 partner studies in 33 countries, including several malaria endemic regions that were previously underrepresented. For first time we include dried blood spot sequenced after selective whole amplification, necessitating new methods to genotype copy number variations. We identify a large newly emerging...
In 2021, nationwide malaria molecular surveillance revealed a high prevalence of validated artemisinin resistance marker, the kelch13 (k13) Arg561His mutation, in Kagera region northwestern Tanzania. We aimed to investigate efficacy artemether-lumefantrine and artesunate-amodiaquine confirm presence partial (ART-R) Karagwe district this region. This single-arm, therapeutic study was carried out at Bukangara dispensary Eligible participants were aged between 6 months 120 months, had confirmed...
Plasmodium falciparum with the histidine rich protein 2 gene (pfhrp2) deleted from its genome can escape diagnosis by HRP2-based rapid diagnostic tests (HRP2-RDTs). The World Health Organization (WHO) recommends switching to a non-HRP2 RDT for P. clinical case when pfhrp2 deletion prevalence causes ≥ 5% of RDTs return false negative results. Tanzania is country heterogenous transmission, some regions approaching elimination and others at varying levels control. In concordance current...
Abstract Background Despite implementation of effective interventions in the past two decades, malaria is still a major public health problem Tanzania. This study assessed prevalence and drivers infections among symptomatic asymptomatic members selected communities from five regions with varying endemicity mainland Methods A cross-sectional community survey was conducted districts, including one district/region Kagera, Kigoma, Njombe, Ruvuma Tanga July to August 2023. Participants aged ≥ 6...
Ebb and flow of parasite populations The population genetics the malaria Plasmodium falciparum across Africa is poorly understood but important to know for grasping risks dynamics spread drug resistance. Harnessing power genomics, Amambua-Ngwa et al. Diversity Network found substantial structure within that consistent with human vector divergence (see Perspective by Sibley). Specific signatures selection antimalarial drugs were detected, along indications effect colonization slavery....
Abstract The Democratic Republic of the Congo (DRC) harbors 11% global malaria cases, yet little is known about spatial and genetic structure parasite population in that country. We sequence 2537 Plasmodium falciparum infections, including a nationally representative sample from DRC samples surrounding countries, using molecular inversion probes - high-throughput genotyping tool. identify an east-west divide haplotypes to confer resistance chloroquine sulfadoxine-pyrimethamine. Furthermore,...
Abstract Background Emergence of artemisinin partial resistance (ART-R) in Plasmodium falciparum is a growing threat to the efficacy combination therapies (ACT) and efforts for malaria elimination. The emergence Kelch13 (K13) R561H Rwanda raised concern about impact neighboring Tanzania. In addition, regional over affecting sulfadoxine-pyrimethamine (SP), which used chemoprevention strategies, high. Methods To enhance longitudinal monitoring, Molecular Surveillance Malaria Tanzania (MSMT)...
Abstract In Africa, the first Plasmodium falciparum Kelch13 (K13) artemisinin partial resistance mutation 561H was detected and validated in Rwanda. Surveillance to better define extent of emergence Rwanda neighboring countries as other mutations arise East Africa is critical. We employ a novel scheme liquid blood drop preservation combined with pooled sequencing provide cost-effective rapid assessment frequencies at multiple collection sites across countries. Malaria-positive samples...
Abstract Background Although Tanzania adopted and has been implementing effective interventions to control eventually eliminate malaria, the disease is still a leading public health problem, country experiences heterogeneous transmission. Recent studies reported emergence of parasites with artemisinin partial resistance (ART-R) in Kagera region high prevalence (> 10.0%) two districts Karagwe Kyerwa. This study assessed predictors/risk malaria infections among asymptomatic individuals...
Abstract Background Artemisinin-based combination therapies (ACTs) are the recommended antimalarial drugs for treatment of uncomplicated malaria. The recent emergence artemisinin partial resistance (ART-R) in Rwanda, Uganda and Eritrea is great concern. In Tanzania, a nationwide molecular malaria surveillance 2021 showed high prevalence Kelch13 (K13) 561H mutation Plasmodium falciparum from north-western region, close to border with Rwanda Uganda. This study was conducted 2022 evaluate...
Abstract Background Recent studies point to the need incorporate detection of non-falciparum species into malaria surveillance activities in sub-Saharan Africa, where 95% world’s cases occur. Although caused by infection with Plasmodium falciparum is typically more severe than P. malariae , ovale spp. and vivax latter may be challenging diagnose, treat, control ultimately eliminate. The prevalence throughout Africa poorly defined. Tanzania has geographical heterogeneity transmission levels...
Abstract Background In Africa, the first Plasmodium falciparum artemisinin partial resistance mutation was Kelch13 (K13) 561H, detected and validated at appreciable frequency in Rwanda 2014. Surveillance to better define extent of emergence neighboring countries is critical. Methods We used novel liquid blood drop preservation with pooled sequencing provide cost-effective rapid assessment frequencies multiple collection sites across regions Uganda, Tanzania, Democratic Republic Congo....
Despite some problems related to accuracy and applicability of malaria rapid diagnostic tests (RDTs), they are currently the best option in areas with limited laboratory services for improving case management through parasitological diagnosis reducing over-treatment. This study was conducted declining burden assess; 1) RDTs when used at different community settings, 2) impact using on anti-malarial dispensing by community-owned resource persons (CORPs) 3) adherence CORPs treatment guidelines...
Artemisinin-based combination therapy (ACT) is the first-line anti-malarial treatment of uncomplicated malaria in most endemic countries, including Tanzania. Unfortunately, there have been reports artemisinin resistance and ACT failure from South East Asia highlighting need to monitor therapeutic efficacy these countries as recommended by World Health Organization.Open-label single arm studies mainland Tanzania were conducted nine sentinel sites 2011, 2012 2015 assess safety...
Abstract Background The World Health Organization recommends regular therapeutic efficacy studies (TES) to monitor the performance of first and second-line anti-malarials. In 2016, safety artemether-lumefantrine (AL) for treatment uncomplicated falciparum malaria were assessed through a TES conducted between April October 2016 at four sentinel sites Kibaha, Mkuzi, Mlimba, Ujiji in Tanzania. study also molecular markers artemisinin lumefantrine (partner drug) resistance. Methods Eligible...
High-throughput Plasmodium genomic data is increasingly useful in assessing prevalence of clinically important mutations and malaria transmission patterns. Understanding parasite diversity for identification specific human or populations that can be targeted by control programmes, to monitor the spread associated with drug resistance. An up-to-date understanding regional population dynamics also critical impact efforts. However, this largely absent from high-burden nations Africa, date, no...
<ns3:p>MalariaGEN is a data-sharing network that enables groups around the world to work together on genomic epidemiology of malaria. Here we describe new release curated genome variation data 7,000 <ns3:italic>Plasmodium falciparum</ns3:italic> samples from MalariaGEN partner studies in 28 malaria-endemic countries. High-quality genotype calls 3 million single nucleotide polymorphisms (SNPs) and short indels were produced using standardised analysis pipeline. Copy number variants associated...
Recent data indicate that non-Plasmodium falciparum species may be more prevalent than thought in sub-Saharan Africa. Although Plasmodium malariae, ovale spp., and vivax are less severe P. falciparum, treatment control challenging, their geographic distributions not well characterized. We randomly selected 3284 of 12 845 samples collected from cross-sectional surveys 100 health facilities across 10 regions Mainland Tanzania performed quantitative real-time PCR to determine presence...