Mark M. Fukuda
A5075351189- Malaria Research and Control
- Mosquito-borne diseases and control
- Computational Drug Discovery Methods
- Drug-Induced Hepatotoxicity and Protection
- Influenza Virus Research Studies
- HIV/AIDS drug development and treatment
- Trypanosoma species research and implications
- Viral Infections and Vectors
- Viral Infections and Outbreaks Research
- Travel-related health issues
- Zoonotic diseases and public health
- Drug Transport and Resistance Mechanisms
- Immune Response and Inflammation
- Vector-borne infectious diseases
- Hemoglobinopathies and Related Disorders
- Neonatal Health and Biochemistry
- Research on Leishmaniasis Studies
- Epilepsy research and treatment
- HIV Research and Treatment
- Data-Driven Disease Surveillance
- Animal Disease Management and Epidemiology
- HIV, Drug Use, Sexual Risk
- Infectious Encephalopathies and Encephalitis
- interferon and immune responses
- Pharmaceutical Quality and Counterfeiting
Armed Forces Research Institute of Medical Science
2013-2024
University of Oxford
2023
Centre for Human Genetics
2023
Institute of Medical Sciences
2017
University of Florida
2015
Armed Forces Health Surveillance Center
2011-2013
United States Army
2007-2010
Centre National de la Recherche Scientifique
2009
Hôpital de la Timone
2009
Medical University of Vienna
2006-2007
The recent emergence of artemisinin-resistant Plasmodium falciparum malaria in western Cambodia could threaten prospects for elimination. Identification the genetic basis resistance would provide tools molecular surveillance, aiding efforts to contain resistance. Clinical trials artesunate efficacy were conducted Bangladesh, northwestern Thailand near Myanmar border, and at two sites Cambodia. Parasites collected from trial participants genotyped 8,079 single nucleotide polymorphisms (SNPs)...
MalariaGEN is a data-sharing network that enables groups around the world to work together on genomic epidemiology of malaria. Here we describe new release curated genome variation data 7,000 Plasmodium falciparum samples from partner studies in 28 malaria-endemic countries. High-quality genotype calls 3 million single nucleotide polymorphisms (SNPs) and short indels were produced using standardised analysis pipeline. Copy number variants associated with drug resistance structural cause...
<ns3:p>We describe the MalariaGEN Pf7 data resource, seventh release of <ns3:italic>Plasmodium falciparum</ns3:italic> genome variation from network. It comprises over 20,000 samples 82 partner studies in 33 countries, including several malaria endemic regions that were previously underrepresented. For first time we include dried blood spot sequenced after selective whole amplification, necessitating new methods to genotype copy number variations. We identify a large newly emerging...
Background. Increasing rates of failure artemisinin-based combination therapy have highlighted the possibility emerging artemisinin resistance along Thai-Cambodian border. We used an integrated in vivo-in vitro approach to assess presence western Cambodia. This article provides additional data from a clinical trial that has been published The New England Journal Medicine. Methods. Ninety-four adult patients Battambang Province, Cambodia, who presented with uncomplicated falciparum malaria...
Malaria elimination will be possible only with serious attempts to address asymptomatic infection and chronic by both Plasmodium falciparum vivax. Currently available drugs that can completely clear a human of P. vivax (known as "radical cure"), reduce transmission malaria parasites, are those in the 8-aminoquinoline drug family, such primaquine. Unfortunately, people glucose-6-phosphate dehydrogenase (G6PD) deficiency risk having severe adverse reactions if exposed these at certain doses....
Abstract Human gingival fibroblasts (HGFs), a predominant cell type in tooth-supporting structure, are presently recognized for their active role the innate immune response. They produce variety of inflammatory cytokines response to microbial components such as LPS from key periodontal pathogen, Porphyromonas gingivalis. In this study, we demonstrated that HGFs expressed mRNA TLRs 1, 2, 3, 4, 5, 6, and 9, but not 7, 8, 10. Stimulation with highly purified TLR2 ligand (P. gingivalis LPS),...
Amplified copy number in the plasmepsin II/III genes within Plasmodium falciparum has been associated with decreased sensitivity to piperaquine. To examine this association and test whether additional loci might also contribute, we performed a genome-wide study of ex vivo P. susceptibility DNA from 183 samples collected primarily Cambodia was genotyped at 33716 single nucleotide polymorphisms (SNPs). Linear mixed models random forests were used estimate associations between parasite...
A simple double-site sandwich enzyme-linked immunosorbent assay (ELISA) for Plasmodium falciparum in vitro drug sensitivity tests based on measuring histidine-rich protein 2 (HRP2) is presented. The ELISA uses two commercial monoclonal antibodies and provides a drastically cheaper alternative to the test kits previously used HRP2 test. establish perform. comparable results very closely match those obtained with (R(2) = 0.979; P < 0.001; mean log difference at 50% inhibitory concentration 0.07).
Background The emergence of artemisinin resistance has raised concerns that the most potent antimalarial drug may be under threat. currently recommended daily dose artesunate (AS) is 4 mg/kg, and administered for 3 days together with a partner drug. This study investigated impact different AS doses on clinical parasitological responses in malaria patients from an area known western Cambodia. Methods Adult uncomplicated P. falciparum were randomized into one three 7-day monotherapy regimens:...
<ns3:p>MalariaGEN is a data-sharing network that enables groups around the world to work together on genomic epidemiology of malaria. Here we describe new release curated genome variation data 7,000 <ns3:italic>Plasmodium falciparum</ns3:italic> samples from MalariaGEN partner studies in 28 malaria-endemic countries. High-quality genotype calls 3 million single nucleotide polymorphisms (SNPs) and short indels were produced using standardised analysis pipeline. Copy number variants associated...
Abstract There is worldwide concern that the avian influenza H5N1 virus, with a mortality rate of &gt;50%, might cause next pandemic. Unlike most other infections, infection causes systemic disease. The underlying mechanisms for this effect are still unclear. In study, we investigate interplay between and human dendritic cells (DC). We showed virus can infect replicate in monocyte-derived blood myeloid DC, leading to cell death. These results suggest escapes viral-specific immunity,...
Human gingival epithelial cells (HGECs) are continually exposed to oral bacteria and other harmful agents. Their responses stimuli critical in maintaining periodontal homeostasis. The aim of this study was investigate the modulating effect cigarette smoke extract (CSE) on innate immune HGECs.Toll-like receptor (TLR) expression HGECs determined by reverse transcriptase-polymerase chain reaction (RT-PCR). CSE or nicotine antimicrobial peptide human beta-defensin-2 (hBD-2) pro-inflammatory...
Estimates of Plasmodium falciparum migration may inform strategies for malaria elimination. Here we elucidate fine-scale parasite population structure and infer recent across Southeast Asia using identity-by-descent (IBD) approaches based on genome-wide single nucleotide polymorphisms called in 1722 samples from 54 districts. IBD estimates are consistent with isolation-by-distance. We observe greater sharing larger segments between artemisinin-resistant parasites versus sensitive parasites,...
A hospital-based study was conducted along the Thai-Myanmar border to provide greater knowledge of causes febrile illness and determine what zoonotic vector-borne emerging infectious diseases might be present. total 613 adults were enrolled from June 1999 March 2002. Cases classified based on clinical findings laboratory results. An etiologic diagnosis made for 48% subjects. Malaria most common diagnosis, accounting 25% subjects, with two-thirds Plasmodium falciparum. Serologic evidence...
Because antimalarial drug resistance is spreading, there an urgent need for new combination treatments malaria, which kills >1 million people every year. Azithromycin a macrolide antibiotic that particularly attractive as because of its safety in children and the extensive experience with use during pregnancy.We undertook randomized, controlled, 28-day inpatient trial involving patients acute, uncomplicated Plasmodium falciparum malaria. We compared efficacy 2 azithromycin-artesunate...
Enzyme-linked immunosorbent assays (ELISAs) allow for the testing of large numbers samples within a short time frame. We tested sensitivity and specificity histidine-rich protein 2 (HRP2)-based, commercially available ELISA antigen detection assay Plasmodium falciparum (Malaria Antigen CELISA; Cellabs, Sydney, Australia). A total 700 whole blood obtained from symptomatic outpatients malaria clinics along Thai–Myanmar border were relative to blinded duplicate expert microscopy adjusted with...
Intrinsic resistance of Plasmodium falciparum is clearly a major determinant the clinical failure antimalarial drugs. However, complex interactions between host, parasite and drug obscure ability to define in vivo. The vitro susceptibility assay determines ex-vivo growth presence serial concentrations and, thus, eliminates host effects, such as metabolism immunity. Although sensitivity various antimalarials provided by test provides an important indicator intrinsic susceptibility, there are...
Fears of emerging artemisinin resistance in western Cambodia have prompted a series clinical trials investigating whether slow responses to antimalarial treatment can be overcome by increasing doses drug.Patients with uncomplicated malaria were allocated 1 3 oral artesunate monotherapy regimens (2, 4, or 6 mg/kg/day for 7 days) and observed 42 days. A safety measures, including complete blood count on days 0, 3, 6, 14, was implemented because lack data these experimental doses.After doses,...
Despite widespread coverage of the emergence artemisinin resistance, relatively little is known about parasite populations responsible. The use PCR genotyping around highly polymorphic Plasmodium falciparum msp1, msp2 and glurp genes has become well established both to describe variability in alleles within a population parasites, as classify treatment outcome cases recurrent disease. primary objective was assess minority clones during seven days artesunate (AS) location with resistance. An...
ABSTRACT Azithromycin when used in combination with faster-acting antimalarials has proven efficacious treating Plasmodium falciparum malaria phase 2 clinical trials. The aim of this study was to establish optimal ratios for azithromycin either dihydroartemisinin or quinine, determine the correlates vitro drug sensitivity these compounds, and assess cross-sensitivity patterns. Seventy-three fresh P. isolates originating from patients western border regions Thailand were successfully tested...