A5029116837- Mosquito-borne diseases and control
- Malaria Research and Control
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- Vector-borne infectious diseases
- Computational Drug Discovery Methods
- Genomics and Phylogenetic Studies
- COVID-19 epidemiological studies
- Zoonotic diseases and public health
- Bacteriophages and microbial interactions
- Viral Infections and Outbreaks Research
- Microbial infections and disease research
- Travel-related health issues
- Animal Disease Management and Epidemiology
- Drug-Induced Hepatotoxicity and Protection
- Complement system in diseases
- Animal Virus Infections Studies
- Biosensors and Analytical Detection
- Viral gastroenteritis research and epidemiology
- COVID-19 Clinical Research Studies
- Cardiac electrophysiology and arrhythmias
- Dengue and Mosquito Control Research
- Research on Leishmaniasis Studies
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 detection and testing
National Institutes of Health
2018-2024
National Institute of Allergy and Infectious Diseases
2018-2024
Vector (United States)
2022-2024
Ministry of Health
2024
Virginia Commonwealth University
2023
Cambodia National Malaria Center
2022
National Institute of Biomedical Imaging and Bioengineering
2022
Frederick National Laboratory for Cancer Research
2022
University of New Hampshire
2021
Government of the United States of America
2021
Metagenomic next-generation sequencing (mNGS) has enabled the rapid, unbiased detection and identification of microbes without pathogen-specific reagents, culturing, or a priori knowledge microbial landscape. mNGS data analysis requires series computationally intensive processing steps to accurately determine composition sample. Existing tools typically require bioinformatics expertise access local server-class hardware resources. For many research laboratories, this presents an obstacle,...
SignificanceMetagenomic pathogen sequencing offers an unbiased approach to characterizing febrile illness. In resource-scarce settings with high biodiversity, it is critical identify disease-causing pathogens in order understand burden and prioritize efforts for control. Here, metagenomic next-generation (mNGS) characterization of the landscape Cambodia revealed diverse vector-borne zoonotic irrespective age gender as risk factors. Identification key led changes national program...
The prevalence and epidemiology of important viral (equine influenza virus [EIV], equine herpesvirus type 1 [EHV-1] EHV-4) bacterial (Streptococcus equi subspecies equi) respiratory pathogens shed by horses presented to veterinarians with upper tract signs and/or acute febrile neurological disease were studied. Veterinarians from throughout the USA enrolled in a surveillance programme asked collect blood nasal secretions cases infectious onset disease. A questionnaire was used information...
Introduction Emerging antimalarial drug resistance in mobile populations remains a significant public health concern. We compared two regimens of dihydroartemisinin-piperaquine military and civilians on the Thai-Cambodian border to evaluate national treatment policy. Methods Efficacy safety three-day were as nested open-label evaluation within malaria cohort study 222 otherwise healthy volunteers (18% malaria-infected at baseline). The first 80 with slide-confirmed Plasmodium falciparum or...
Global dengue incidence has increased dramatically over the past few decades from approximately 500 000 reported cases in 2000 to 5 million 2019. This trend been attributed population growth endemic areas, rapid unplanned urbanization, increasing global connectivity, and climate change expanding geographic range of
Despite widespread coverage of the emergence artemisinin resistance, relatively little is known about parasite populations responsible. The use PCR genotyping around highly polymorphic Plasmodium falciparum msp1, msp2 and glurp genes has become well established both to describe variability in alleles within a population parasites, as classify treatment outcome cases recurrent disease. primary objective was assess minority clones during seven days artesunate (AS) location with resistance. An...
Dihydroartemisinin-piperaquine, the current first-line drug for uncomplicated malaria caused by Plasmodium falciparum and vivax in Cambodia, was previously shown to be of benefit as chemoprophylaxis when administered a monthly 3-day regimen. We sought evaluate protective efficacy compressed 2-day treatment course Royal Cambodian Armed Forces. The safety dosing regimen dihydroartemisinin-piperaquine were evaluated two-arm, randomized, double-blind, placebo-controlled cohort study with 2:1...
Inhabitants of the Greater Mekong Subregion in Cambodia are exposed to pathogens that might influence serologic cross-reactivity with severe acute respiratory syndrome coronavirus 2. A prepandemic serosurvey 528 malaria-infected persons demonstrated higher-than-expected positivity nonneutralizing IgG spike and receptor-binding domain antigens. These findings could affect interpretation large-scale serosurveys.
Mosquito-borne viruses are a growing global threat. Initial viral inoculation occurs in the skin via mosquito 'bite', eliciting immune responses that shape establishment of infection and pathogenesis. Here we assess cutaneous innate adaptive to controlled Aedes aegypti feedings humans living Aedes-endemic areas. In this single-arm, cross-sectional interventional study (trial registration #NCT04350905), enroll 30 healthy adult participants aged 18 45 years age from Cambodia between October...
Abstract Rapid production and publication of pathogen genome sequences during emerging disease outbreaks provide crucial public health information. In resource-limited settings, especially near an outbreak epicenter, conventional deep sequencing or bioinformatics are often challenging. Here we successfully used metagenomic next generation on iSeq100 Illumina platform paired with open-source pipeline to quickly characterize Cambodia’s first case COVID-2019.
Long-term antimalarial chemoprophylaxis is currently used by deployed U.S. military personnel. Previous small, short-term efficacy studies have shown variable rates of side effects among patients taking various forms chemoprophylaxis, though reliable safety and tolerability data on long-term use are limited. We conducted a survey troops returning to Fort Drum, NY following 12-month deployment Operation Enduring Freedom, Afghanistan from 2006 2007. Of the 2,351 respondents, 95% reported at...
ABSTRACT Background Metagenomic next generation sequencing (mNGS) has enabled the rapid, unbiased detection and identification of microbes without pathogen-specific reagents, culturing, or a priori knowledge microbial landscape. mNGS data analysis requires series computationally intensive processing steps to accurately determine composition sample. Existing tools typically require bioinformatics expertise access local server-class hardware resources. For many research laboratories, this...
Abstract Background We established the first prospective cohort to understand how infection with dengue virus is influenced by vector-specific determinants such as humoral immunity Aedes aegypti salivary proteins. Methods Children aged 2–9 years were enrolled in PAGODAS (Pediatric Assessment Group of Dengue and Saliva) informed consent their guardians. followed semi-annually for antibodies proteins Ae. gland homogenate using enzyme-linked immunosorbent assays dengue-specific neutralization...
Metagenomic next-generation sequencing (mNGS) is the process of all genetic material in a biological sample. The technique growing popularity with myriad applications including outbreak investigation, biosurveillance, and pathogen detection clinical samples. However, mNGS programs are costly to build maintain, additional obstacles faced by low- middle-income countries (LMICs) may further widen global inequities capacity. Over past two decades, several important infectious disease outbreaks...
The host response to infection by Plasmodium falciparum, the parasite most often responsible for severe malaria, ranges from asymptomatic parasitaemia death. clinical trajectory of malaria is influenced genetics and load, but factors determining why some infections produce uncomplicated proceed disease remain incompletely understood. To identify molecular markers falciparum human gene expression patterns were compared between children aged 6 months 5 years with who enrolled in a case–control...
The incidence of dengue virus disease has increased globally across the past half-century, with highest number cases ever reported in 2019 and again 2023. We analyzed climatological, epidemiological, phylogenomic data to investigate drivers two decades Cambodia, an understudied endemic setting. Using epidemiological models fit a 19-y dataset, we first demonstrate that climate-driven transmission alone is insufficient explain three epidemics time series. then use wavelet decomposition...
ABSTRACT Despite the rising rates of resistance to dihydroartemisinin-piperaquine (DP), DP remains a first-line therapy for uncomplicated malaria in many parts Cambodia. While is generally well tolerated as 3-day (3DP) regimen, compressed 2-day (2DP) regimens were associated with treatment-limiting cardiac repolarization effects recent clinical trial. To better estimate risks piperaquine on QT interval prolongation, we pooled data from three randomized trials conducted between 2010 and 2014...
Aedes mosquitoes are some of the most important and globally expansive vectors disease. Public health efforts largely focused on prevention human-vector contact. A range entomological indices used to measure risk disease, though with conflicting results (i.e. larval or adult abundance does not always predict disease). There is a growing interest in development use biomarkers for exposure mosquito saliva, including spp, as proxy disease risk. In this study, we conduct comprehensive...
Southeast Asia (SEA) emerged relatively unscathed from the first year of global SARS-CoV-2 pandemic, but as July 2021 region is experiencing a surge in case numbers primarily driven by Alpha (B.1.1.7) and subsequently more transmissible Delta (B.1.617.2) variants. While initial disease burden was mitigated swift government responses, favorable cultural societal factors, recent rise cases suggests an under-appreciation prior prevalence over-appreciation possible cross-protective immunity...