A5070867013- Rheumatoid Arthritis Research and Therapies
- Systemic Lupus Erythematosus Research
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Spondyloarthritis Studies and Treatments
- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Malaria Research and Control
- Immunotherapy and Immune Responses
- Vector-borne infectious diseases
- Viral Infections and Outbreaks Research
- Lymphoma Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Viral Infections and Immunology Research
- Chemokine receptors and signaling
- Inflammatory mediators and NSAID effects
- Systemic Sclerosis and Related Diseases
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Inflammatory Myopathies and Dermatomyositis
- Psoriasis: Treatment and Pathogenesis
- Insect symbiosis and bacterial influences
- Immune Response and Inflammation
Institut Pasteur du Cambodge
2017-2025
New York Academy of Sciences
2023
John Wiley & Sons (Germany)
2023
Moss Landing Marine Laboratories
2023
Hudson Institute
2023
Yale University
2012-2016
Amsterdam UMC Location University of Amsterdam
2006-2016
University of Amsterdam
2006-2016
KU Leuven
2016
University of Regensburg
2013
IgG fucosylation predicts dengue severity Secondary infections with virus (DENV) can produce life-threatening symptoms, including thrombocytopenia and hemorrhagic disease, when preexisting DENV-reactive immunoglobulin G1 (IgG1) antibodies promote the infection of immune cells. Although severe symptoms are associated increased levels afucosylated IgG1 glycoforms, it is unclear whether this simply a result or if phenomenon that dictate susceptibility to disease. Bournazos et al. studied Fab Fc...
Anti-citrullinated protein antibodies have been detected with high specificity in serum of patients rheumatoid arthritis (RA), and citrullination proteins may play a key role the pathogenesis RA. We therefore investigated presence two extra-articular manifestations RA, interstitial pneumonia (IP) nodules.Open-lung biopsy specimens from RA-associated IP (n = 18), idiopathic 20) controls 10), as well nodules 26 patients, were examined. All sections incubated an anti-modified citrulline...
Abstract Objective Peripheral spondylarthritis (SpA) is characterized by macrophages that express CD163, a marker of alternative activation (M2). The purpose this study was to assess whether differential infiltration with macrophage subsets associated different local inflammatory milieu in SpA as compared rheumatoid arthritis (RA). Methods effect and RA synovial fluid (SF) on polarization tested vitro normal peripheral blood monocytes. SF levels classically activated (M1)–derived...
Common variable immune deficiency (CVID) is an assorted group of primary diseases that clinically manifest with antibody deficiency, infection susceptibility, and autoimmunity. Heterozygous mutations in the gene encoding tumor necrosis factor receptor superfamily member TACI are associated CVID autoimmune manifestations, whereas two mutated alleles prevent To assess how number affects B cell activation tolerance checkpoints, we analyzed healthy individuals patients carrying one or mutations....
Multiple sclerosis (MS) is a genetically mediated autoimmune disease of the central nervous system. B cells have recently emerged as major contributors to pathogenesis, but mechanisms responsible for loss cell tolerance in patients with MS are largely unknown. In healthy individuals, developing autoreactive removed from repertoire at 2 checkpoints during early development. Both these and peripheral defective rheumatoid arthritis (RA) type 1 diabetes (T1D). Here, we found that only...
Objective To profile quantitatively the T-cell repertoire in multiple joints and peripheral blood of patients with recent onset (early) or established rheumatoid arthritis (RA) using a novel next-generation sequencing protocol to identify potential autoreactive clones. Methods Synovium RA (<6 months) (n=6) (>18 was screened for clones by over 10 000 receptors (TCR) per sample. T cells from paired samples were analysed comparison. From two synovial obtained inflamed joints. The degree...
Experimental and human data suggest that tumour necrosis factor (TNF) blockade may affect B cell responses, in particular the induction of T cell-dependent (TD) humoral immunity. This study aimed to assess this hypothesis directly patients with arthritis by analysing longitudinally effect TNF on activation maturation responses against TD cell-independent vaccines.Peripheral blood samples were obtained from 56 spondyloarthritis before after treatment either non-steroidal anti-inflammatory...
<b>Background:</b> Ectopic lymphoid neogenesis (LN) occurs in rheumatoid synovium, where it is thought to drive local antigen-dependent B cell development and autoantibody production. This process involves the expression of specific homing chemokines high endothelial venules (HEV). <b>Objective:</b> To investigate whether these mechanisms occur psoriatic arthritis (PsA) autoantibodies have not been described organisation function cells clear, analyse their clinical correlates....
Abstract Objective To address the ongoing debate concerning specificity of synovial citrullinated proteins for rheumatoid arthritis (RA) and to analyze their pathophysiologic relevance induction or perpetuation RA‐specific anti–citrullinated protein antibodies (ACPAs). Methods Synovium 19 RA patients non‐RA controls was immunostained presence with a mouse monoclonal antibody (F95), citrullinating enzyme peptidyl arginine deiminase type 2 (PAD‐2), free citrulline–producing inducible nitric...
Cross-regulation between TNF and type I IFN has been postulated to play an important role in autoimmune diseases. Therefore, we determined the effect of blockade rheumatoid arthritis (RA) on response gene activity relation clinical response.Peripheral blood from 33 RA patients was collected PAXgene tubes before after start infliximab treatment. In a first group 15 baseline expression IFN-regulated genes using cDNA microarrays compared levels one month The remaining 18 were studied as...
To investigate the relationship between lymphoid neogenesis in synovium of patients with rheumatoid arthritis (RA) and characteristics inflammation disease severity.Arthroscopic synovial biopsy was performed 103 active RA (Disease Activity Score 28-joint assessment >or=3.2) who had not received treatment biologic agents. Sections were stained assessed by digital image analysis. Lymphocyte aggregates counted graded for size (1-3). Synovial defined as presence grade 2 or 3 subclassified based...
Abstract B lymphocyte autoimmunity plays a crucial role in the pathogenesis of rheumatoid arthritis. The local production autoantibodies and presence ectopic lymphoid neogenesis synovium suggest that these dedicated microenvironments resembling canonical follicles may regulate initiation maturation cell autoimmunity. In this study, we assessed experimentally relevance for by detailed structural, molecular, serological analysis seropositive seronegative human synovitis. We demonstrate...
Type 1 diabetes (T1D) patients show abnormalities in early B cell tolerance checkpoints, resulting the accumulation of large numbers autoreactive cells their blood. Treatment with rituximab, an anti-CD20 mAb that depletes cells, has been shown to preserve β function T1D and improve other autoimmune diseases, including rheumatoid arthritis multiple sclerosis. However, it remains largely unknown how anti-B therapy thwarts autoimmunity these pathologies. Here, we analyzed reactivity Abs...
Dengue virus (DENV) induces strong T and B cell responses upon infection. Hence, it is difficult to determine the contribution of cell-mediated immunity alone in long lasting protection against DENV infection disease. Numerous CD4+ CD8+ epitopes have been identified, mainly non-structural proteins DENV. Taking into account immunogenicity peptide sequence conservation among different serotypes, a minimal antigen, called DENV1-NS, has designed. This antigen enriched conserved highly antigenic...
Dengue is the most prevalent arthropod-borne viral disease. Clinical symptoms of dengue virus (DENV) infection range from classical mild fever to severe, life-threatening shock syndrome. However, DENV infections cause few or no symptoms. Asymptomatic DENV-infected patients provide a unique opportunity decipher host immune responses leading elimination without negative impact on t v 'health. We used an integrated approach transcriptional profiling and immunological analysis comparing...
We studied the diagnostic performance of anti-human citrullinated fibrinogen antibody (AhFibA) ELISA for rheumatoid arthritis (RA) in a consecutive cohort (population 1) and evaluated agreement between AhFibA four other assays anti-citrullinated protein/peptide antibodies (ACPAs) as well factor patients with longstanding RA 2). Population 1 consisted 1024 rheumatic symptoms; serum samples from these were sent to our laboratory ACPA testing within context investigation RA. Ninety-two...
Abstract Objective Spondylarthritis (SpA) and rheumatoid arthritis (RA) have different patterns of bone damage, with more pronounced erosions in RA. The RANK/RANKL/osteoprotegerin (OPG) system plays a central role resorption by promoting the maturation activation osteoclasts. To assess potential this distinct phenotype, we studied synovial expression these mediators SpA RA peripheral synovitis. Methods Synovial biopsy specimens were obtained from actively inflamed joints 35 patients 19...
Objectives. To investigate the presence and characteristics of citrullinated vimentin in protein extracts inflamed synovial tissue. Methods. Cytosolic obtained from RA (n = 14) SpA patients were analysed by gel electrophoresis western blotting. Citrullinated isoforms visualized a combination anti-modified citrulline (AMC) staining anti-vimentin detections (V9, H-84). This was subsequently confirmed immunoprecipitation. Autoantibody detection verified using sera form 6) patients. Results. A...
Objective. It remains unclear whether autoantibodies are useful biomarkers to tailor the choice of biological treatment in RA. We investigated relationship between presence and levels different RF ACPA isotypes response TNF blockade an exploratory study. Methods. A total 101 active RA patients were prospectively treated with infliximab (3 mg/kg). Changes disease activity monitored by 28-joint DAS (DAS-28). Serum [immunoglobulins M, G (IgM, IgG IgA)] anti-citrullinated peptide antibodies...
Abstract Objective The molecular processes driving the distinct patterns of synovial inflammation and tissue remodeling in spondylarthritis (SpA) as compared to rheumatoid arthritis (RA) remain largely unknown. Therefore, we aimed identify novel unsuspected disease‐specific pathways SpA by a systematic unbiased gene expression analysis. Methods Differentially expressed genes were identified pan‐genomic microarray confirmed quantitative polymerase chain reaction immunohistochemical analyses...
Patients with mutations in AICDA, which encodes activation-induced cytidine deaminase (AID), display an impaired peripheral B cell tolerance. AID mediates class-switch recombination (CSR) and somatic hypermutation (SHM) cells, but the mechanism by prevents accumulation of autoreactive cells blood is unclear. Here, we analyzed tolerance AID-deficient patients, patients autosomal dominant (AD-AID), asymptomatic AICDA heterozygotes (AID+/–), uracil N-glycosylase (UNG) deficiency, impairs CSR...