A5061539308- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Influenza Virus Research Studies
- Rabies epidemiology and control
- Malaria Research and Control
- Viral Infections and Outbreaks Research
- Virology and Viral Diseases
- Animal Disease Management and Epidemiology
- COVID-19 epidemiological studies
- Zoonotic diseases and public health
- Respiratory viral infections research
- Viral gastroenteritis research and epidemiology
- Data-Driven Disease Surveillance
- Yersinia bacterium, plague, ectoparasites research
- SARS-CoV-2 and COVID-19 Research
- Microbial infections and disease research
- Dengue and Mosquito Control Research
- Streptococcal Infections and Treatments
- Insect symbiosis and bacterial influences
- Long-Term Effects of COVID-19
- Bacillus and Francisella bacterial research
- Salmonella and Campylobacter epidemiology
- Pneumonia and Respiratory Infections
- Computational Drug Discovery Methods
- COVID-19 Clinical Research Studies
Institut Pasteur du Cambodge
2016-2025
Hôpital Saint-André
2023
Scotland's Rural College
2022
Australian Institute of Tropical Health and Medicine
2018
James Cook University
2018
Federation University
2018
Ministry of Health
2012-2017
Cambodia National Malaria Center
2016
Institut Pasteur
2007-2015
National Institute for Nanotechnology
2010
DrugBank ( http://www.drugbank.ca ) is a richly annotated database of drug and target information. It contains extensive data on the nomenclature, ontology, chemistry, structure, function, action, pharmacology, pharmacokinetics, metabolism pharmaceutical properties both small molecule large (biotech) drugs. also comprehensive information diseases, proteins, genes organisms which these drugs act. First released in 2006, has become widely used by pharmacists, medicinal chemists, researchers,...
Significance Our work provides evidence that people who are infected with dengue virus without developing detectable clinical symptoms or prior to the onset of infectious mosquitoes. At a given level viremia, symptom-free were markedly more mosquitoes than clinically symptomatic patients. results fundamentally change current paradigm for epidemiology and control, based on detection virus-infected cases apparent illness.
IgG fucosylation predicts dengue severity Secondary infections with virus (DENV) can produce life-threatening symptoms, including thrombocytopenia and hemorrhagic disease, when preexisting DENV-reactive immunoglobulin G1 (IgG1) antibodies promote the infection of immune cells. Although severe symptoms are associated increased levels afucosylated IgG1 glycoforms, it is unclear whether this simply a result or if phenomenon that dictate susceptibility to disease. Bournazos et al. studied Fab Fc...
Background Detection of dengue NS1 antigen in acute infection has been proposed for early diagnosis disease. The aim this study was to evaluate the clinical and virological factors influencing performance Platelia Ag kit (BioRad) assess potential use viral loads as markers disease severity. Methodology/Principal Findings Blood specimens were collected from patients hospitalized at Kampong Cham hospital during 2006 2007 epidemics Cambodia. Dengue confirmed 243/339 symptomatic 17 asymptomatic...
Tens of thousands people die from dog-mediated rabies annually. Deaths can be prevented through post-exposure prophylaxis for who have been bitten, and the disease eliminated dog vaccination. Current use saves many lives, but availability remains poor in rabies-endemic countries due to high costs, access, supply.
Dengue laboratory diagnosis is essentially based on detection of the virus, its components or antibodies directed against virus in blood samples. Blood, however, may be difficult to draw some patients, especially children, and sampling during outbreak investigations epidemiological studies face logistical challenges limited compliance invasive procedures from subjects. The aim this study was assess possibility using saliva urine samples instead for dengue diagnosis.Serial plasma, were...
Background Dengue vaccines are now in late-stage development, and evaluation robust estimates of dengue disease burden needed to facilitate further development introduction. In Cambodia, the national case-definition only allows reporting children less than 16 years age, little is known about rural areas among older persons. To estimate true largest province Kampong Cham, we conducted community-based active fever surveillance 0-to-19–year age group villages urban during 2006–2008. Methods...
Background Disease incidence data are needed to guide decision-making for public health interventions. Although dengue is a reportable disease in Thailand and Cambodia, the degree that reported underrecognizes true burden unknown. We utilized calculated from laboratory-confirmed outpatient inpatient cases prospective cohort studies estimate magnitude of underrecognition establish more accurate estimates these countries. Methods Findings Cohort were conducted among children aged <15 years by...
Background Rabies, a fatal but preventable zoonosis, is major public health problem in developing countries. In Cambodia the disease burden largely underestimated because patients with encephalitis following dog bites are rarely hospitalized and die at home. Since 1998 Institut Pasteur (IPC), Phnom Penh has been only source of free post-exposure prophylaxis (PEP) post-mortem diagnosis. Methods The 1998–2007 data compiled by IPC was analyzed to describe all treated for PEP, results human...
We conducted investigations in 2 villages Cambodia where outbreaks of influenza H5N1 occurred among humans and poultry to determine the frequency risk factors for virus transmission.During May 2006, approximately 7 weeks after occurred, villagers living near households patients with were interviewed about potential exposures had blood samples obtained serological testing by microneutralization assay. A seropositive result was defined as an neutralizing antibody titer 1:80, confirmation...
Wet markets are common in many parts of the world and may promote emergence, spread maintenance livestock pathogens, including zoonoses. A survey was conducted order to assess potential Vietnamese Cambodian live bird (LBMs) sustain circulation highly pathogenic avian influenza virus subtype H5N1 (HPAIV H5N1). Thirty 8 LBMs were visited, structured interviews with market managers 561 84 traders. Multivariate cluster analysis used construct a typology traders based on their poultry management...
Dengue is the most prevalent arthropod-borne viral disease. Clinical symptoms of dengue virus (DENV) infection range from classical mild fever to severe, life-threatening shock syndrome. However, DENV infections cause few or no symptoms. Asymptomatic DENV-infected patients provide a unique opportunity decipher host immune responses leading elimination without negative impact on t v 'health. We used an integrated approach transcriptional profiling and immunological analysis comparing...
Southeastern Asia is a recognised hotspot for emerging infectious diseases, many of which have an animal origin. Mammarenavirus infections contribute significantly to the human disease burden in both Africa and Americas, but little data exists Asia. To date only two mammarenaviruses, widely spread lymphocytic choriomeningitis virus recently described Wēnzhōu been identified this region, zoonotic impact remains unknown. Here we report presence novel mammarenavirus genetic variant provide...
Abstract We describe a retrospective study on circulation of Zika virus in Cambodia during 2007–2016 among patients with dengue-like symptoms and Aedes aegypti mosquitoes. Our findings suggest that belongs to the Asia genotype, is endemic, has low prevalence, had low-level impact public health.
Surveillance for avian influenza viruses (AIVs) in poultry and environmental samples was conducted four live-bird markets Cambodia from January through November 2013. Through real-time RT-PCR testing, AIVs were detected 45% of 1048 collected throughout the year. Detection rates ranged 32% 18% duck chicken swabs, respectively, to 75% carcass wash water samples. Influenza A/H5N1 virus 79% positive A 35% all collected. Sequence analysis full-length haemagglutinin (HA) neuraminidase (NA) genes...
Abstract To determine potential risk for bird-to-human transmission during influenza A virus (H5N1) outbreaks among backyard poultry in rural Cambodia, we collected environmental specimens. Viral RNA was detected 27 (35%) of 77 specimens mud, pond water, water plants, and soil swabs. Our results underscore the need regular disinfection areas.
Abstract Because avian influenza H5N1 infection risks are associated with exposure to infected poultry, we conducted a knowledge, attitudes, and practices survey of poultry-handling behavior among villagers in rural Cambodia. Despite widespread knowledge personal protection measures, most Cambodians still have high level at-risk poultry handling.
SUMMARY Robust disease burden estimates are important for decision-making concerning introduction of new vaccines. Dengue is a major public health problem in the tropics but robust lacking. We conducted two-sample, capture–recapture study largest province Cambodia to determine under-recognition National Surveillance System (NDSS). During 2006–2008, community-based active surveillance acute febrile illness was 0- 19-year-olds rural and urban areas combined with testing dengue virus infection....
Following almost 30 years of relative silence, chikungunya fever reemerged in Kenya 2004. It subsequently spread to the islands Indian Ocean, reaching Southeast Asia 2006. The virus was first detected Cambodia 2011 and a large outbreak occurred village Trapeang Roka Kampong Speu Province March 2012, which 44% villagers had recent infection biologically confirmed. epidemic curve constructed from number biologically-confirmed CHIKV cases per day determined date onset, self-reported during data...
The East/Central/South African genotype of Chikungunya virus with the E1-A226V mutation emerged in 2011 Cambodia and spread 2012. An outbreak 190 cases was documented Trapeang Roka, a rural village. We surveyed 425 village residents within 3-4 weeks after outbreak, determined sensitivity specificity case definitions factors associated infection by CHIKV. Self-reported clinical presentation consisted mostly fever, rash arthralgia. presence all three signs or symptoms identified as most...
Longitudinal surveillance of 2 live bird markets in Cambodia revealed year-round, high co-circulation H5, H7, and H9 influenza viruses. We detected A viruses 51.3% ducks 39.6% chickens, co-infections, mainly by H5 viruses, 0.8% 4.5% chickens.
Chikungunya virus (CHIKV) is an alphavirus circulating worldwide. Its presence in Asia has been reported since the 1950s, constituting Asian genotype. Since 2005, strains from Eastern, Central, and Southern African (ECSA) genotype have caused several outbreaks across Asia. Viruses ECSA were also detected Cambodia late 2011 led to outbreak a rural community 2012. A former investigation 2012 found higher risk of infection people younger than 40 years, suggesting pre-existing herd immunity...
The pathogenesis of dengue involves a complex interplay between the viral factor and host immune response. A mismatch infecting serotype adaptive memory response is hypothesized to lead exacerbated responses resulting in severe dengue. Here, we aim define detail phenotype function different regulatory T cell (Treg) subsets their association with disease severity cohort acute virus (DENV)-infected Cambodian children. Treg frequencies proliferation Tregs are increased patients compared...