A5007422098- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- IL-33, ST2, and ILC Pathways
- Lymphatic System and Diseases
- Immune cells in cancer
- Monoclonal and Polyclonal Antibodies Research
- Eosinophilic Esophagitis
- Retinoids in leukemia and cellular processes
- Rheumatoid Arthritis Research and Therapies
- Reproductive System and Pregnancy
- CAR-T cell therapy research
- Cancer Immunotherapy and Biomarkers
- Pancreatic and Hepatic Oncology Research
- Glycosylation and Glycoproteins Research
- Neonatal Respiratory Health Research
- Angiogenesis and VEGF in Cancer
- Cancer Cells and Metastasis
- Chemokine receptors and signaling
- Single-cell and spatial transcriptomics
- Atherosclerosis and Cardiovascular Diseases
- Neuroinflammation and Neurodegeneration Mechanisms
- Platelet Disorders and Treatments
Vrije Universiteit Amsterdam
2014-2025
Amsterdam University Medical Centers
2018-2025
Netherlands Center for Occupational Diseases
2025
Cancer Center Amsterdam
2018-2025
University of Amsterdam
1987-2024
Academic Center for Dentistry Amsterdam
2024
Oncode Institute
2023-2024
Amsterdam UMC Location Vrije Universiteit Amsterdam
2011-2023
Amsterdam Institute for Addiction Research
2022
Amsterdam UMC Location University of Amsterdam
2017
Most developing thymocytes undergo apoptosis because they cannot interact productively with molecules encoded by the major histocompatibility complex. Here, we show that mice lacking orphan nuclear hormone receptor RORγ lose thymic expression of anti-apoptotic factor Bcl-xL. thus regulates survival CD4 + 8 and may control temporal window during which can positive selection. was also required for development lymph nodes Peyer's patches, but not splenic follicles. In its absence, there loss a...
The incidence of food allergies in western countries has increased dramatically recent decades. Tolerance to antigens relies on mucosal CD103(+) dendritic cells (DCs), which promote differentiation regulatory T (Treg) cells. We show that high-fiber feeding mice improved oral tolerance and protected from allergy. High-fiber reshaped gut microbial ecology the release short-chain fatty acids (SCFAs), particularly acetate butyrate. enhanced against allergy by enhancing retinal dehydrogenase...
For more than a decade, the biological roles and apparent redundancy of cytokines tumor necrosis factor (TNF) lymphotoxin (LT) have been debated. LT alpha exists in its soluble form as homotrimer, which like TNF only binds receptors, TNF-R55 or TNF-R75. The cell surface heteromer beta subunits this complex specifically receptor (LT beta-R). To discriminate functions systems, beta-R-immunoglobulin (Ig) TNF-R-Ig fusion proteins were introduced into embryonic circulation by injecting pregnant...
Atherosclerosis involves a macrophage-rich inflammation in the aortic intima. It is increasingly recognized that this intimal paralleled over time by distinct inflammatory reaction adjacent adventitia. Though cross talk between coordinated foci intima and adventitia seems implicit, mechanism(s) underlying their communication unclear. Here, using detailed imaging analysis, microarray analyses, laser-capture microdissection, adoptive lymphocyte transfers, functional blocking studies, we...
Abstract The gastrointestinal tract is continuously exposed to many environmental factors that influence intestinal epithelial cells and the underlying mucosal immune system. In this article, we demonstrate dietary fiber short chain fatty acids (SCFAs) induced expression of vitamin A–converting enzyme RALDH1 in vivo vitro, respectively. Furthermore, our data showed levels small correlated with activity enzymes mesenteric lymph node dendritic cells, along increased numbers regulatory T a...
Abstract Despite recent advances in cancer immunotherapy, pancreatic ductal adenocarcinoma (PDAC) remains unresponsive due to an immunosuppressive tumor microenvironment, which is characterized by the abundance of cancer-associated fibroblasts (CAFs). Once identified, CAF-mediated immune inhibitory mechanisms could be exploited for immunotherapy. Siglec receptors are increasingly recognized as checkpoints, and their ligands, sialic acids, known overexpressed cells. Here, we unveil a...
Proper lymph node (LN) development requires tumor necrosis factor-related activation-induced cytokine (TRANCE) expression. Here we demonstrate that the defective LN in TRANCE(-/)- mice correlates with a significant reduction lymphotoxin (LT)alphabeta(+)alpha(4)beta(7)(+)CD45(+)CD4(+)CD3(-) cells and their failure to form clusters rudimentary mesenteric LNs. Transgenic TRANCE overexpression results selective restoration of this cell population into clusters, full development. TRANCE-mediated...
Abstract We identified an IL-7Rα+Sca-1lowc-Kitlow population in E14 fetal liver, which is the phenotypical analog of common lymphoid progenitors (CLP) adult bone marrow. After transfer into newborn mice, rapidly differentiated CD45+CD4+CD3− cells, are candidate cells for initiating lymph node and Peyer’s patch formation. In addition, this also gave rise to B, T, NK, CD8α+ CD8α− dendritic cells. The liver precursors expressed a significantly lower level myeloid-suppressing transcription...
IN adult mice, the dominant adhesion molecules involved in homing to lymph nodes are L-selectin receptors on lymphocytes and peripheral node addressins specialized high endothelial venules. Here we show that, from fetal life through first 24 hr of life, mucosal addressin MAdCAM-1 venules its counterreceptor, Peyer's patch receptor, integrin alpha 4 beta 7 circulating cells. Before birth, 40-70% blood leukocytes L-selectin-positive, while only 1-2% expresses 7. However, preferentially attract...
Access to the splenic white pulp is restricted lymphocytes and dendritic cells. Here we show that movement of molecules from blood into these confined areas also limited. Large molecules, such as bovine serum albumin (68 kD), immunoglobulin G (150 500 kD dextran are unable enter pulp, whereas smaller blood-borne can directly permeate this compartment. The distribution a stromal network refer conduit system. small lumen contains collagen fibers surrounded in T cell by reticular fibroblasts...
Abstract The interaction between TNFR family member CD27 and its ligand CD70 promotes lymphocyte expansion effector cell formation. In humans, control of function is partly regulated by the restricted expression CD70. We used newly developed mAbs to characterize murine (m) in vitro vivo. On resting lymphocytes immature dendritic cells (DC), mCD70 absent. vitro, Ag receptor triggering induced mRNA T cells, but surface protein was very low. Activated B synthesized much higher levels than...
The blood–brain barrier (BBB) is crucial in the maintenance of a controlled environment within brain to safeguard optimal neuronal function. endothelial cells (ECs) BBB possess specific properties that restrict entry and metabolites into CNS. specialized phenotype induced during neurovascular development by surrounding However, molecular differentiation endothelium remains poorly understood. Retinoic acid (RA) plays role embryogenesis. Because radial glial supply with RA developmental...