A5045424398- Immunotherapy and Immune Responses
- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Carbohydrate Chemistry and Synthesis
- vaccines and immunoinformatics approaches
- Escherichia coli research studies
- HIV Research and Treatment
- Ubiquitin and proteasome pathways
- Immune cells in cancer
- Pancreatic and Hepatic Oncology Research
- Cellular Mechanics and Interactions
- Platelet Disorders and Treatments
- RNA Interference and Gene Delivery
- Cancer Research and Treatments
- Antimicrobial Peptides and Activities
- Drug Transport and Resistance Mechanisms
- Parasites and Host Interactions
- RNA modifications and cancer
- Helminth infection and control
- Bacteriophages and microbial interactions
Amsterdam University Medical Centers
2018-2024
Vrije Universiteit Amsterdam
2008-2024
Cancer Center Amsterdam
2020-2024
Amsterdam UMC Location Vrije Universiteit Amsterdam
2011-2023
Amsterdam Institute for Addiction Research
2023
Oncode Institute
2020-2022
Amsterdam UMC Location University of Amsterdam
2019-2020
National Medical Research Center of Dentistry and Maxillofacial Surgery
1999-2016
Amsterdam Neuroscience
2015
Radboud University Nijmegen
1997-2002
Mycobacterium tuberculosis represents a world-wide health risk and immunosuppression is particular problem in M. infections. Although macrophages are primarily infected, dendritic cells (DCs) important inducing cellular immune responses against tuberculosis. We hypothesized that DCs represent target for the observed immuno-suppression results from modulation of DC functions. demonstrate DC-specific C-type lectin DC-SIGN an receptor on captures internalizes intact bovis bacillus...
Abstract Dendritic cells (DCs) capture Ags or viruses in peripheral tissue to transport them lymphoid organs induce cellular T cell responses. Recently, a DC-specific C-type lectin was identified, ICAM-grabbing non-integrin (DC-SIGN), that functions as adhesion receptor mediating both DC migration and activation. DC-SIGN also an HIV-1R captures HIVgp120 facilitates DC-induced HIV transmission of cells. Internalization motifs the cytoplasmic tail hint function endocytic receptor. In this...
Dendritic cells (DC) are instrumental in handling pathogens for processing and presentation to T cells, thus eliciting an appropriate immune response. C-type lectins expressed by DC function as pathogen-recognition receptors; yet their specificity carbohydrate structures on is not fully understood. In this study, we analyzed the of DC-specific ICAM-3-grabbing nonintegrin (SIGN)/CD209, recently documented HIV-1 receptor DC. Our studies show that DC-SIGN binds with high affinity both synthetic...
Human cholangiocytes are continuously exposed to millimolar levels of hydrophobic bile salt monomers. We recently hypothesized that an apical biliary HCO Symbol umbrella might prevent the protonation glycine–conjugated salts and uncontrolled cell entry corresponding acids, defects in this predispose chronic cholangiopathies. Here, we tested vitro whether human cholangiocyte integrity presence monomers is dependent on (1) pH, (2) adequate expression key exporter, anion exchanger 2 (AE2), (3)...
Changes in glycosylation during tumour progression are a key hallmark of cancer. One the glycan moieties generally overexpressed cancer sialic acids, which can induce immunomodulatory properties via binding to Siglec receptors. We here show that Pancreatic Ductal Adenocarcinoma (PDAC) cells present an increased sialylation be recognized by Siglec-7 and Siglec-9 on myeloid cells. identified expression α2,3 sialyltransferases ST3GAL1 ST3GAL4 as main contributor synthesis ligands for Analysing...
Abstract Despite recent advances in cancer immunotherapy, pancreatic ductal adenocarcinoma (PDAC) remains unresponsive due to an immunosuppressive tumor microenvironment, which is characterized by the abundance of cancer-associated fibroblasts (CAFs). Once identified, CAF-mediated immune inhibitory mechanisms could be exploited for immunotherapy. Siglec receptors are increasingly recognized as checkpoints, and their ligands, sialic acids, known overexpressed cells. Here, we unveil a...
The discovery of dendritic cell (DC)-specific intercellular adhesion molecule (ICAM)-3–grabbing nonintegrin (DC-SIGN) as a DC-specific ICAM-3 binding receptor that enhances HIV-1 infection T cells in trans has indicated potentially important role for molecules AIDS pathogenesis. A related called DC-SIGNR exhibits 77% amino acid sequence identity with DC-SIGN. DC-SIGN and genes map within 30-kb region on chromosome 19p13.2-3. Their strong homology close physical location indicate recent...
The human gastric pathogen Helicobacter pylori spontaneously switches lipopolysaccharide (LPS) Lewis (Le) antigens on and off (phase-variable expression), but the biological significance of this is unclear. Here, we report that Le+ H. variants are able to bind C-type lectin DC-SIGN present dendritic cells (DCs), demonstrate interaction blocks T helper cell (Th)1 development. In contrast, Le− escape binding DCs induce a strong Th1 response. addition, in biopsies challenged ex vivo with...
A new polysaccharide secreted by the human opportunistic fungal pathogen Aspergillus fumigatus has been characterized. Carbohydrate analysis using specific chemical degradations, mass spectrometry, 1H and 13C nuclear magnetic resonance showed that this is a linear heterogeneous galactosaminogalactan composed of α1-4 linked galactose N-acetylgalactosamine residues where both monosacharides are randomly distributed percentage per chain varied from 15 to 60%. This antigenic recognized majority...
Dendritic cells (DCs) are key to the maintenance of peripheral tolerance self-antigens and orchestration an immune reaction foreign antigens. C-type lectins, expressed by DCs, recognize carbohydrate moieties on antigens that can be internalized for processing presentation. Little is known about exact glycan structures pathogens specifically recognized different lectins how this interaction influences DC function. We have analyzed specificity human lectin macrophage galactose-type (MGL) using...
ABSTRACT DCs orchestrate immune responses to infectious pathogens and disturbances in tissue integrity. Equipped with C-type lectins, can respond environmental changes glycosylation. Many lectins are capable of modulating TLR activation, thereby facilitating tailor-made reactions. Here, we investigated the signaling properties lectin MGL show that engagement by agonistic antibodies or carbohydrate ligands couples signal transduction for increased IL-10 TNF-α secretion human monocyte-derived...
Glioblastoma is the most aggressive brain malignancy, for which immunotherapy has failed to prolong survival. Glioblastoma-associated immune infiltrates are dominated by tumor-associated macrophages and microglia (TAMs), key mediators of suppression resistance immunotherapy. We others demonstrated aberrant expression glycans in different cancer types. These trigger inhibitory signaling TAMs through glycan-binding receptors. investigated glioblastoma glycocalyx as a tumor-intrinsic...
Changes in glycosylation patterns have been associated with malignant transformation and clinical outcomes several cancer types, prompting ongoing research into the mechanisms involved potential applications. In this study, we performed an extensive transcriptomic analysis of glycosylation-related genes pathways, using publicly available bulk single cell datasets from tumor samples lines. We identified pathways strongly different which may represent novel diagnostic biomarkers. By RNA-seq...
The novel dendritic cell (DC)-specific human immunodeficiency virus type 1 (HIV-1) receptor DC-SIGN plays a key role in the dissemination of HIV-1 by DC. is thought to capture at mucosal sites entry, facilitating transport lymphoid tissues, where efficiently transmits T cells. also important initiation immune responses regulating DC-T interactions through intercellular adhesion molecule 3 (ICAM-3). We have characterized mechanism ligand binding and identified crucial amino acids involved...