A5045892228- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral gastroenteritis research and epidemiology
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Parvovirus B19 Infection Studies
- Viral Infections and Immunology Research
- Plant Virus Research Studies
- Bacterial Identification and Susceptibility Testing
- Polyomavirus and related diseases
- Antibiotic Resistance in Bacteria
- Biosensors and Analytical Detection
- SARS-CoV-2 detection and testing
- Coral and Marine Ecosystems Studies
- Aquaculture disease management and microbiota
- Respiratory viral infections research
- Turtle Biology and Conservation
- Aquaculture Nutrition and Growth
- Herpesvirus Infections and Treatments
- COVID-19 epidemiological studies
- Microbial Community Ecology and Physiology
- Metalloenzymes and iron-sulfur proteins
- Spacecraft and Cryogenic Technologies
- SARS-CoV-2 and COVID-19 Research
Berkeley College
2024
Chan Zuckerberg Initiative (United States)
2022-2024
Nova Southeastern University
2024
University of California, San Francisco
2019-2024
McMaster University
2024
University of California, Berkeley
2024
University of KwaZulu-Natal
2022
Stellenbosch University
2022
Vitalant
2019-2020
Pacific Research Institute
2019-2020
Significance Sea stars inhabiting the Northeast Pacific Coast have recently experienced an extensive outbreak of wasting disease, leading to their degradation and disappearance from many coastal areas. In this paper, we present evidence that cause disease is transmissible disease-affected animals apparently healthy individuals, disease-causing agent a virus-sized microorganism, best candidate viral taxon, sea star-associated densovirus (SSaDV), in greater abundance diseased than stars.
Using viral metagenomics of brain tissue from a young adult crossbreed steer with acute onset neurologic disease, we sequenced the complete genome novel astrovirus (BoAstV-NeuroS1) that was phylogenetically related to an ovine astrovirus. In retrospective analysis 32 cases bovine encephalitides unknown etiology, 3 other infected animals were detected by using PCR and in situ hybridization for RNA. Viral RNA restricted nervous system cytoplasm affected neurons within spinal cord, brainstem,...
The viruses circulating among Antarctic wildlife remain largely unknown. In an effort to identify associated with Weddell seals (Leptonychotes weddellii) inhabiting the Ross Sea, vaginal and nasal swabs, faecal samples were collected between November 2014 February 2015. addition, a seal kidney South Polar skua (Stercorarius maccormicki) faeces opportunistically sampled. Using high throughput sequencing, we identified recovered 152 anellovirus genomes that share 63–70% genome-wide identities...
Feces from dogs in an unexplained outbreak of diarrhea were analyzed by viral metagenomics revealing the genome a novel parvovirus. The parvovirus was named cachavirus and classified within proposed Chapparvovirus genus. Using PCR, DNA detected two nine tested that outbreak. In order to begin elucidate clinical impact this virus, 2,053 canine fecal samples screened using real-time PCR. Stool 203 healthy positive for at rate 1.47%, while 802 collected 2017 964 2018 rates 4.0% 4.66%...
Plasma from patients with dengue-like symptoms was collected in 2013 to 2016 the Brazilian states of Tocantins and Amapa. 781 samples testing negative for IgM against Dengue, Zika, Chikungunya viruses flaviviruses, alphaviruses enteroviruses RNA using RT-PCRs were analyzed viral metagenomics. Viral particles-associated nucleic acids enriched, randomly amplified, deep sequenced 102 mini-pools generating over 2 billion reads. Sequence data presence known novel eukaryotic Anelloviruses detected...
Chemolithoautotrophic bacteria from the genera Hydrogenovibrio, Thiomicrorhabdus and Thiomicrospira are common, sometimes dominant, isolates sulfidic habitats including hydrothermal vents, soda salt lakes marine sediments. Their genome sequences confirm their membership in a deeply branching clade of Gammaproteobacteria. Several adaptations to heterogeneous apparent. genomes include large numbers genes for sensing responding environment (EAL- GGDEF-domain proteins methyl-accepting chemotaxis...
Chelonid alphaherpesvirus 5 (ChHV5) is a herpesvirus associated with fibropapillomatosis (FP) in sea turtles worldwide. Single-locus typing has previously shown differentiation between Atlantic and Pacific strains of this virus, low variation within each geographic clade. However, lack multi-locus genomic sequence data hinders understanding the rate mechanisms ChHV5 evolutionary divergence, as well how these changes may contribute to differences disease manifestation. To assess among five...
Cynomolgus macaques are common across South East Asian countries including Thailand. The National Primate Research Center of Thailand, Chulalongkorn University (NPRCT-CU) captures wild-borne cynomolgus macaque for research use. Limited information is available on the enteric viruses and possible zoonotic infections into or from macaques. We characterized compare fecal virome two populations; healthy wild-originated captive (n = 43) reared in NPRCT-CU wild 35). Over 90% recognized viral...
Metagenomic next-generation sequencing (mNGS) is the process of all genetic material in a biological sample. The technique growing popularity with myriad applications including outbreak investigation, biosurveillance, and pathogen detection clinical samples. However, mNGS programs are costly to build maintain, additional obstacles faced by low- middle-income countries (LMICs) may further widen global inequities capacity. Over past two decades, several important infectious disease outbreaks...
A viral metagenomic analysis of feces from an unexplained outbreak feline diarrhea revealed the presence Lyon-IARC polyomavirus (LIPyV) DNA. LIPyV, whose genome was originally sequenced swabs human skin, fecally shed by three out five diarrheic cats.
Viral infections were investigated in American black bears ( Ursus americanus ) from Nevada and northern California with without idiopathic encephalitis. Metagenomics analyses of tissue pools revealed novel viruses the genera Circoviridae , Parvoviridae Anelloviridae Polyomaviridae Papillomaviridae . The circovirus parvovirus particular interest due to their potential importance as pathogens. We characterized genomes these subsequently screened by PCR determine prevalence. (Ursus circovirus,...
All of the known porcine sapeloviruses (PSVs) currently belong to a single genotype in genus Sapelovirus (family Picornaviridae). Here, complete genome second, possibly recombinant, PSV strain SZ1M-F/PSV/HUN2013 (MN807752) from faecal sample paraplegic pig Hungary was characterized using viral metagenomics and RT-PCR. This sapelovirus showed only 64 % nucleotide identity VP1 region its closest PSV-1 relative. Complete sequence-based epidemiological investigations PSVs circulating presence...
Antimicrobial resistant (AMR) pathogens represent urgent threats to human health, and their surveillance is of paramount importance. Metagenomic next generation sequencing (mNGS) has revolutionized such efforts, but remains challenging due the lack open-access bioinformatics tools capable simultaneously analyzing both microbial AMR gene sequences. To address this need, we developed Chan Zuckerberg ID (CZ ID) module, an open-access, cloud-based workflow designed integrate detection microbes...
<title>Abstract</title> Antimicrobial resistant (AMR) pathogens represent urgent threats to human health, and their surveillance is of paramount importance. Metagenomic next generation sequencing (mNGS) has revolutionized such efforts, but remains challenging due the lack open-access bioinformatics tools capable simultaneously analyzing both microbial AMR gene sequences. To address this need, we developed CZ ID module, an open-access, cloud-based workflow designed integrate detection...
In this study, the full length genomes of three phylogenetically distant picornaviruses (family Picornaviridae) belonging to genus Rosavirus (rat08/rRoB/HUN, MN116648), Kobuvirus (rat08/rAiA/HUN, MN116647), and Cardiovirus (rat08/rCaB/HUN, MN116646) were obtained from a single faecal sample free-living Norway rat (Rattus norvegicus) in Hungary using viral metagenomics RT-PCR/Sanger sequencing. The acquired complete silico analyzed detail revealing presence second minor open reading frame...
Abstract New diseases in marine animals are emerging at an increasing rate, yet methodological limitations hinder characterization of viral infections. Viral metagenomics is effective method for identifying novel viruses diseased animals; however, determining virus pathogenesis remains a challenge. A anellovirus ( Zalophus californianus anellovirus, ZcAV) was recently reported the lungs captive California sea lions involved mortality event. ZcAV not detected by PCR blood these animals,...