A5085283841- Influenza Virus Research Studies
- Respiratory viral infections research
- Gut microbiota and health
- Animal Disease Management and Epidemiology
- Hepatitis B Virus Studies
- MicroRNA in disease regulation
- Viral gastroenteritis research and epidemiology
- Liver Disease Diagnosis and Treatment
- Hepatitis C virus research
- Viral Infections and Vectors
- interferon and immune responses
- Diet and metabolism studies
- Viral Infections and Immunology Research
- RNA Interference and Gene Delivery
- Mosquito-borne diseases and control
- SARS-CoV-2 detection and testing
- Clostridium difficile and Clostridium perfringens research
- Animal Virus Infections Studies
- CRISPR and Genetic Engineering
- Dermatology and Skin Diseases
- Cervical Cancer and HPV Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Advanced biosensing and bioanalysis techniques
- Biosensors and Analytical Detection
Chulalongkorn University
2016-2025
Center for Systems Biology
2023
King Chulalongkorn Memorial Hospital
2012
Thai Red Cross Society
2012
Bangkok University
2010
Thailand Center of Excellence in Physics
2010
Centers for Disease Control and Prevention
2008
Abstract Influenza virus is not known to affect wild felids. We demonstrate that avian influenza A (H5N1) caused severe pneumonia in tigers and leopards fed on infected poultry carcasses. This finding extends the host range of has implications for epidemiology wildlife conservation.
Avian influenza H5N1 virus is known to cross the species barrier and infect humans felines.We report a fatal infection in dog following ingestion of an H5N1-infected duck during outbreak Thailand 2004.With new reports continuing across Asia, Europe, Africa, this finding highlights need for monitoring domestic animals outbreaks.H ighly pathogenic avian (HPAI) has spread Africa.Not limited poultry, also been shown infecting (1) felines, including cats (2) tigers (3-5).Both were reported as...
Abstract We report H5N1 virus infection in a domestic cat infected by eating pigeon carcass. The isolated from the and showed same cluster as viruses obtained during outbreak Thailand. Since cats are common house pets, concern regarding disease transmission to humans exists.
Abstract During the second outbreak of avian influenza H5N1 in Thailand, probable horizontal transmission among tigers was demonstrated tiger zoo. Sequencing and phylogenetic analysis those viruses showed no differences from first isolate obtained January 2004. This finding has implications for virus epidemiology pathogenicity mammals.
Abstract In 2004, canine influenza virus subtype H3N8 emerged in greyhounds the United States. Subsequent serologic evidence indicated circulation dog breeds other than greyhounds, but had not been isolated from affected animals. 2005, we conducted virologic investigation of 7 nongreyhound dogs that died respiratory disease Florida and virus. Antigenic genetic analysis A/canine/Jacksonville/2005 (H3N8) A/canine/Miami/2005 found similarity to earlier isolates which indicates viruses are...
To determine the prevalence of human rhinoviruses (HRV) infections in children with lower respiratory disease Thailand and monitor association between species HRV clinical presentation hospitalized paediatric patients.Two hundred eighty-nine nasopharyngeal (NP) suction specimens were collected from patients admitted to King Chulalongkorn Memorial Hospital, during February 2006-2007. Nucleic acids extracted each sample subsequent amplification VP4/2 by semi-nested RT-PCR for detection. Other...
Background: Non-Alcoholic Fatty Liver Disease (NAFLD) is an over accumulation of triglyceride in the liver without alcohol consumption. Its major cause insulin resistance. Patients with NAFLD can develop fibrosis, cirrhosis and hepatocellular carcinoma (HCC). MicroRNAs (miRNAs) are non-coding RNAs that regulate post-transcriptional gene silencing. Previous research reported miR-29 family (a, b c) miR-122 have important role regulating resistance related to NAFLD. Objective: The purpose this...
When severe, COVID-19 shares many clinical features with bacterial sepsis. Yet, secondary infection is uncommon. However, as epithelium injured and barrier function lost, products entering the circulation might contribute to pathophysiology of COVID-19. We studied 19 adults, severely ill patients infection, who were admitted King Chulalongkorn Memorial Hospital, Bangkok, Thailand, between 13th March 17th April 2020. Blood samples on days 1, 3, 7 enrollment analyzed for endotoxin activity...
Due to the common symptoms of COVID-19, patients are similar influenza-like illness. Therefore, detection method would be crucial discriminate between SARS-CoV-2 and influenza virus-infected patients. In this study, CRISPR-Cas12a-based was applied for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), A virus, B virus which a practical attractive application screening with COVID-19 in areas limited resources. The limit SARS-CoV-2, A, 10, 10 3 , copies/reaction, respectively....
Background One of the key barriers preventing rapid diagnosis leptospirosis is lack available sensitive point-of-care testing. This study aimed to develop and validate a clustered regularly-interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein 12a (CRISPR/Cas12a) platform combined with isothermal amplification detect leptospires from extracted patient DNA samples. Methodology/Principal findings A Recombinase Polymerase Amplification (RPA)-CRISPR/Cas12a-fluorescence assay...
The first publication demonstrating that major depressive disorder (MDD) is associated with alterations in the gut microbiota appeared 2008 (Maes et al., 2008). purpose of present study to delineate a) microbiome signature phenome depression, including suicidal behaviours (SB) and cognitive deficits; effects adverse childhood experiences (ACEs) recurrence illness index (ROI) on microbiome; lowered high-density lipoprotein cholesterol (HDLc). We determined isometric log-ratio abundances or...
Abstract Background & Aims The role of quantitative serum hepatitis B core‐related antigen ( HB crAg) in patients with chronic CHB ) receiving pegylated interferon PEG ‐ IFN is unclear. This study was aimed at comparing its usefulness sAg eAg‐positive therapy. Methods A total 46 treated for 48 weeks were retrospectively analysed. Intrahepatic covalently closed circular DNA (ccc from paired liver biopsies and serial crAg during therapy assessed. Results Virological response VR ), defined...
MicroRNAs (miRNAs) play an important role in the regulation of gene expression and are involved many cellular processes including inhibition viral replication infected cells. In this study, three subtypes influenza A viruses (pH1N1, H5N1 H3N2) were analyzed to identify candidate human miRNAs targeting silencing genes expression. Candidate predicted by miRBase RNAhybrid based on minimum free energy (MFE) hybridization patterns between target genes. silico analysis presented 76 viruses, 70...
Rapid, accurate, and low-cost detection of SARS-CoV-2 is crucial to contain the transmission COVID-19. Here, we present a cost-effective smartphone-based device coupled with machine learning-driven software that evaluates fluorescence signals CRISPR diagnostic SARS-CoV-2. The consists three-dimensional (3D)-printed housing optic components allow excitation fluorescent reporters selective emission smartphone. Custom equipped binary classification model has been developed quantify acquired...
Studies investigating the effect of multispecies synbiotic supplementation in obesity management are limited. The current study was performed to evaluate effects probiotics mixed with fructooligosaccharides on body composition, antioxidant status, and gut microbiome composition overweight obese individuals. We employed a randomized, double-blind, placebo-controlled trial design, which 63 individuals aged 18-45 years were assigned receive either supplement or placebo for 12 weeks. group...
Maes et al. (2008) published the first paper demonstrating that major depressive disorder (MDD) is accompanied by abnormalities in microbiota–gut–brain axis, as evidenced elevated serum IgM/IgA to lipopolysaccharides (LPS) of Gram-negative bacteria, such Morganella morganii and Klebsiella Pneumoniae. The latter aberrations, which point increased gut permeability (leaky gut), are linked activated neuro-immune oxidative pathways MDD. To delineate profile composition microbiome Thai patients...
This study aimed to investigate the effects of gac fruit juice and its probiotic fermentation (FGJ) utilizing Lactobacillus paracasei on modulation gut microbiota production short-chain fatty acids (SCFAs). We conducted a comparison between FGJ, non-fermented (GJ), control samples through in vitro digestion colonic using human derived from fecal inoculum. Our findings revealed that both GJ FGJ led an increase viability Lactobacilli, with exhibiting even higher levels compared control. The...
Abstract Background Edible plants such as Cratoxylum formosum (Jack) Dyer, Curcumin longa Lin, Momordica charantia Lin and Moringa oleifera Lam have long been believed in Thai culture to relieve ulcers the symptoms of liver disease. However, little is known about their anti-liver cancer properties antiviral activity against hepatitis B virus (HBV). The aim this study was investigate anti-HBV activities crude extracts from these edible on human cells. Methods Plant samples were prepared...
MicroRNAs (miRNAs) play an important role in regulation of gene silencing and are involved many cellular processes including inhibition infected viral replication. This study investigated miRNA expression profiles operating response to influenza virus early stage infection which might be useful for understanding control infection. A549 cells were with different subtypes (pH1N1, H3N2 H5N1). After 24 h post-infection, miRNAs extracted then used DNA library construction. All libraries indexes...
Cratoxylum formosum Dyer is a medicinal plant widely found in Asia and commonly consumed for food folk medicine. It rich phenolic compounds. The present study utilized water crude extract of C. leaves to synthesize zinc oxide nanoparticles (ZnO NPs) by green synthesis. synthesized ZnO NPs with the average electronic band gap ∼3 eV were obtained either have spherical shape or sheet-like structures depending on synthesis process concentration extract. Higher also eliminates impurity Zn(OH)2...
Immune dysregulation can involve invasion and survival of endometrial glands inside the myometrium adenomyosis. There is limited available data concerning alterations bacterial microbiome in reproductive tract adenomyosis women. The present cross-sectional age-matched study aims to compare vaginal microbiota between women with without We recruited (N = 40) from Departments Obstetrics Gynaecology, Ramathibodi Hospital Mahidol University, August 2020 January 2021. Vaginal swab samples were...