Osteoarthritis (OA) is a degenerative joint disease leading to pain and stiffness. Due lack of effective treatments, physical psychological disabilities caused by OA have detrimental impact on the patient's quality life. Emerging evidence suggests that intra-articular injection platelet-rich plasma (PRP) may provide favorable results since PRP comprises not only high level platelets but also huge amount cytokines, chemokines, growth factors. However, precise mechanism standardization method...

Studies investigating the effect of multispecies synbiotic supplementation in obesity management are limited. The current study was performed to evaluate effects probiotics mixed with fructooligosaccharides on body composition, antioxidant status, and gut microbiome composition overweight obese individuals. We employed a randomized, double-blind, placebo-controlled trial design, which 63 individuals aged 18-45 years were assigned receive either supplement or placebo for 12 weeks. group...

This study aimed to investigate the effects of gac fruit juice and its probiotic fermentation (FGJ) utilizing Lactobacillus paracasei on modulation gut microbiota production short-chain fatty acids (SCFAs). We conducted a comparison between FGJ, non-fermented (GJ), control samples through in vitro digestion colonic using human derived from fecal inoculum. Our findings revealed that both GJ FGJ led an increase viability Lactobacilli, with exhibiting even higher levels compared control. The...

Osteoarthritis (OA) is a chronic progressive degenerative joint disorder which characterised by strongly age-related regressive changes in articular cartilage. The objective of this study was to evaluate oxidative stress and antioxidant parameters plasma synovial fluid patients with primary knee osteoarthritis.Thirty-five OA 35 healthy controls were recruited for study. Nitrite, malondialdehyde (MDA), vitamin E, Trolox Equivalent Antioxidant Capacity (TEAC), Ferric Reducing Power (FRAP)...

Abstract Postprandial hyperglycemia and hyperlipidemia are key contributors to chronic metabolic disturbances, thereby promoting the onset progression of cardiovascular diseases diabetes. Current dietary interventions aim effectively control postprandial glucose lipid levels while ensuring adequate antioxidant intake. Black rice ( Oryza sativa L.) cultivations have gained attention for their resistant starch content phytochemical compositions. Notably, black is rich in anthocyanins, known...

Abstract High-protein diets have gained popularity for weight management; however, concerns persist regarding increased meat protein intake. This study explored the potential of egg white noodles (EWN) as a novel substitute, utilizing advanced encapsulation techniques to enhance their functional properties. An isocaloric diet with high proportion including 30% replacement by EWN was provided overweight and obese participants 6-week intervention period, 2-week follow-up period without test...

Isoferulic acid (IFA), a naturally occurring cinnamic derivative, is main active ingredient of the rhizoma Cimicifuga dahurica. It has been shown various pharmacological activities. The aim study was to investigate effect IFA against MG-induced protein glycation and oxidative DNA damage. Free radical scavenging activity MGO-trapping abilities were also investigated. fluorescent MG-derived AGEs non-fluorescent Nε-(carboxymethyl) lysine (Nε-CML) measured using spectrofluorometer an enzyme...

Obesity and overweight are consistently associated with metabolic disorders including hyperglycemia hyperlipidemia. Herbal medicines have been currently suggested as an alternative source of potentially useful antihyperglycemic, antihyperlipidemic, antioxidant activities. The objective this study was to assess the in vitro inhibitory effects eleven herbal on carbohydrate lipid digestive enzymes key steps digestion inhibition micelle formation ability bind bile acid. In addition, activity...

This study was performed to evaluate the antioxidative and anti-inflammatory effects of vitamin E on oxidative stress in plasma, synovial fluid, tissue patients with knee osteoarthritis. Seventy-two late-stage osteoarthritis scheduled for total arthroplasty were randomized take oral placebo (Group A) or 400 IU B) once a day 2 months before undergoing surgery. The blood levels endpoints indicating antioxidant capacity, Knee Society Score (KSS), Western Ontario McMaster Universities...

Cyanidin is a natural anthocyanidin present in fruits and vegetables with anti-diabetic properties including stimulation of insulin secretion. However, its mechanism action remains unknown. In this study, we elucidated the mechanisms cyanidin for stimulatory secretion from pancreatic β-cells. Rat β-cells INS-1 were used to investigate effects on secretion, intracellular Ca2+ signaling, gene expression. We detected presence space stimulated increased signals concentration-dependent manner....

Riceberry rice (Oryza sativa L.) is a new pigmented variety of from Thailand. Despite its high anthocyanin content, effect on adipogenesis and adipocyte function remains unexplored. We investigated whether extract (RBE) impacted cell proliferation by examining viability cycle, using preadipocyte 3T3-L1 cells. To test RBE’s formation, cells were cultured in adipogenic medium supplemented with number triglyceride levels quantified. Furthermore, Akt1 phosphorylation along RT-qPCR intracellular...

Cyanidin is the most abundant anthocyanin found in red-purple plants and possesses anti-obesity properties. However, its mechanism of action adipocytes remains unknown. The objective this study was to elucidate how cyanidin inhibits adipocyte formation 3T3-L1 preadipocytes. Cells were cultured adipogenic differentiation medium supplemented with examined for adipogenesis, cell viability, gene expression using Oil Red O staining, MTT assay, RT-qPCR. Real-time Ca2+ imaging analysis performed...

Recently, the mechanisms responsible for anti-glycation activity of cyanidin and its derivatives on inhibition methylglyoxal (MG)-induced protein glycation advanced glycation-end products (AGEs) as well oxidative DNA damage were reported. In this study, we investigated protective effect against MG-induced stress apoptosis in rat INS-1 pancreatic β-cells. Exposure cells to cytotoxic levels MG (500 µM) 12 h caused a significant reduction cell viability. However, pretreatment with alone...

Background: Connective tissue growth factor (CTGF) has been implicated in development of osteoarthritis (OA).Objective: To determine the correlation between plasma and synovial fluid CTGF levels severity knee patients.Methods: A total 100 subjects were recruited into this study (75 OA patients 25 controls). concentrations analyzed by enzyme-linked immunosorbent assay.Results: Plasma correlated with radiographic severity. There was a positive levels.Conclusion: could be useful for monitoring...

Lumbar disc degeneration (LDD) contributes to low back pain. This study aimed determine relative telomere length (RTL), oxidative stress status, and antioxidant levels examine the relationships between RTL, stress, severity in LDD patients. A total of 100 subjects, 50 patients healthy controls, were enrolled case−control study. Blood leukocyte RTL was analyzed using quantitative real-time polymerase chain reaction. Lipid peroxidation determined by malondialdehyde (MDA) assay. Plasma...

Abstract Objectives Investigate the role of transient receptor potential melastatin 4 ( TRPM 4) channel in rat dental pulp stem cell DPSC ) proliferation and survival. Materials methods Immunofluorescence FACS analysis were used to detect marker CD 90. Alizarin Red S Oil O staining identify osteoblast adipocyte differentiation, respectively. To characterize 4, patch‐clamp recordings obtained from single cells whole‐cell configuration mode. The significance for survival was examined with...

Abstract Intracellular Ca 2+ signals are essential for stem cell function and play a significant role in the differentiation process. Dental pulp cells (DPSCs) potential source of cells; however, mechanisms controlling remain largely unknown. Utilizing rat DPSCs, we examined effect adenosine triphosphate (ATP) on osteoblast characterized its mechanism action using real‐time imaging analysis. Our results revealed that ATP enhanced osteogenesis as indicated by deposition extracellular matrix...

Cyanidin-3-rutinoside (C3R) is an anthocyanin with anti-diabetic properties found in red-purple fruits. However, the molecular mechanisms of C3R on Ca2+-dependent insulin secretion remains unknown. This study aimed to identify C3R's action pancreatic β-cells. Rat INS-1 cells were used elucidate effects secretion, intracellular Ca2+ signaling, and gene expression. The results showed that at 60, 100, 300 µM concentrations significantly increased via signaling. exposure up 100 μM did not affect...