A5021597985- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- Virus-based gene therapy research
- Viral Infections and Vectors
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- T-cell and Retrovirus Studies
- Toxoplasma gondii Research Studies
- Effects of Environmental Stressors on Livestock
- Salmonella and Campylobacter epidemiology
- Respiratory viral infections research
- Cancer-related molecular mechanisms research
- Microbial infections and disease research
- Microbial Fuel Cells and Bioremediation
- Cervical Cancer and HPV Research
- Parasitic Infections and Diagnostics
- Bacteriophages and microbial interactions
- Wastewater Treatment and Nitrogen Removal
- RNA Research and Splicing
- Bone Metabolism and Diseases
- RNA modifications and cancer
Oklahoma State University
2019-2025
South Dakota State University
2017-2022
Oklahoma State University Medical Center
2022
Oklahoma State University Center for Health Sciences
2022
Zoetis (United States)
2020
National Animal Disease Center
2012-2020
Ames National Laboratory
2019
Universidade Federal do Rio Grande do Sul
2019
University of California, Davis
2019
Agricultural Research Service
2012-2017
The goal of this study was to evaluate survival important viral pathogens livestock in animal feed ingredients imported daily into the United States under simulated transboundary conditions. Eleven viruses were selected based on global significance and impact industry, including Foot Mouth Disease Virus (FMDV), Classical Swine Fever (CSFV), African (ASFV), Influenza A (IAV-S), Pseudorabies virus (PRV), Nipah (NiV), Porcine Reproductive Respiratory Syndrome (PRRSV), Vesicular (SVDV),...
The emergence of a newly recognized group pestiviruses in cattle, the HoBi-like viruses, requires an evaluation available diagnostic tools and vaccines. present study compared antigenic characteristics Bovine viral diarrhea virus 1 2 (BVDV-1, -2) strains HoBi virus. This comparison was based on detection antibodies against it by commercial enzyme-linked immunosorbent assays (ELISAs) level cross-neutralizing sera from animals vaccinated with BVDV. Reactivity panel monoclonal (mAbs) revealed...
Senecavirus A (SVA), an emerging picornavirus of swine, causes vesicular disease (VD) that is clinically indistinguishable from foot-and-mouth (FMD) in pigs. Many aspects SVA interactions with the host and immune responses to infection, however, remain unknown. In present study, humoral cellular were evaluated following infection We show elicited early robust virus-neutralizing (VN) antibody response, which coincided was strongly correlated VP2- VP3-specific IgM responses. Notably,...
Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic losses to the swine industry worldwide. While PRRSV has been endemic in North America since 1989, it was not until 1999 that first described South America. Notably, recently an increased number of outbreaks have reported American countries. However, epidemiological information related these is limited genetic characteristics strains circulating region are poorly understood. In this study, we describe...
Bovine respiratory disease complex (BRDC) is a widely distributed and multifactorial syndrome, leading to significant economic losses the cattle industry. Many viruses are considered causative agents of BRDC, including bovine herpesvirus 1 (BoHV-1), syncytial virus (BRSV), parainfluenza 3 (PI-3). This study aimed determine seroprevalence BoHV-1, BRSV, PI-3 in serum samples collected from Villavicencio, Colombia. A total 725 animals 29 herds were sampled tested for BoHV-1 BRSV using ELISA....
Malvidin-3-glucoside (M3G), an anthocyanin found in blueberries and grapes, shows promise as a natural anti-diabetic agent. However, its effect on insulin secretion underlying mechanisms remains unclear. This study investigated the impact of M3G β-cells (INS-1) through real-time Ca2+ imaging assays. increased intracellular levels concentration-dependent manner, specifically targeting without affecting other pancreatic cell types. It enhanced under both basal (4 mM) stimulatory (11 glucose...
Abstract Objective —To compare acute infection of cattle exposed to a high-virulence (HV) bovine viral diarrhea virus (BVDV), low-virulence (LV) BVDV, or HoBi-like virus. Animals —24 Holstein bull calves. Procedures —Colostrum-deprived 2- 4-week-old calves, free BVDV antigen and antibodies, were allocated into 4 groups (6 calves/group). Calves in 3 an LV strain (BVDV2-RS886), HV (BVDV2–1373), (D32/00 HoBi), whereas calves the fourth group not but cohoused with Circulating WBCs, platelets,...
Senecavirus A (SVA) is a picornavirus that causes acute vesicular disease (VD), clinically indistinguishable from foot-and-mouth (FMD), in pigs. Notably, SVA RNA has been detected lymphoid tissues of infected animals several weeks following resolution the clinical disease, suggesting virus may persist select host tissues. Here, we investigated occurrence persistent infection and contribution stressors (transportation, immunosuppression, or parturition) to recrudescence infection. Our results...
A infecção pelo Neospora caninum é distribuída mundialmente e tem sido considerada uma importante causa de abortos em bovinos, que são hospedeiros intermediários do protozoário. O presente artigo relata um estudo sorológico da N. 1.024 amostras ovinos bubalinos, oriundos 55 propriedades 16 municípios Estado Rio Grande Sul (RS). Anticorpos contra o agente foram detectados por teste imunoenzimático tipo ELISA 11,4% (89/781) das 14,6% (24/164) dos bubalinos 3,2% (2/62) ovinos. Bovinos...
The detection of an emerging pestivirus species, "HoBi-like virus," in fetal bovine serum (FBS) labeled as U.S. origin, but packaged Europe, raised concerns that HoBi-like virus may have entered the United States. In current study, 90 lots FBS originating North America (NA) were screened for antigen and antibodies. Lots group 1 (G1, 72 samples) 2 (G2, 9 originated NA Group 3 (G3) was composed collected processed Europe. G1 claimed negative Bovine viral diarrhea (BVDV), while G2 G3 positive...
Infection with bovine leukemia virus (BLV) results in economic loss because of reduced productivity, especially milk production, and early culling. In the United States, studies 1996, 1999, 2007 showed BLV infection to be widespread, dairy herds. We updated information herein on seroprevalence using samples submitted for testing found negative antibodies Brucella by Kentucky Eastern Regional Federal Brucellosis Laboratory. From October 2014 through August 2015, 2,000 from all regions...
The present study aimed to describe the bacterial community at an anaerobic up flow bioreactor with ANAMMOX activity, inoculated sludge from pond of a swine slurry treatment system. description was based on molecular DNA techniques using primers for amplification complete 16S rRNA gene and also new amplify smaller fragments rRNA. During operation time, changed significantly, increasing nitrogen removal efficiency, reaching after 500 days rate 94%. PCR generated 17 clones, where three...
ABSTRACT The identification and elimination of persistently infected (PI) cattle are the most effective measures for controlling bovine pestiviruses, including viral diarrhea virus (BVDV) emerging HoBi-like viruses. Here, colostrum-deprived calves with pestivirus (HoBi-like PI calves) were generated sampled (serum, buffy coat, ear notches) on day birth (DOB) weekly 5 consecutive weeks. samples subjected to diagnostic tests BVDV—two reverse transcriptase PCR (RT-PCR) assays, two commercial...
Bovine viral diarrhea virus (BVDV) is an RNA that often associated with respiratory disease in cattle. MicroRNAs have been proposed as indicators of exposure to pathogens. The objective this study was identify microRNAs cattle had challenged a non-cytopathic field strain BVDV. Five colostrum deprived neonate Holstein calves were inoculated BVDV (challenged) and 4 mock (control). Serum from all collected at four different times: prior challenge (day 0) 4, 9, 16 days post-challenge. extracted...
Influenza D virus has been detected predominantly in cattle from several countries. In the United States, regional and state seropositive rates for influenza have previously reported, but little information exists to evaluate national seroprevalence. We performed a serosurveillance study with 1,992 bovine serum samples collected across country 2014 2015. found high overall rate of 77.5% nationally; varied 47.7% 84.6%. Samples Upper Midwest Mountain West regions showed highest rates....