A5040552985- Ion Channels and Receptors
- Pancreatic function and diabetes
- Natural Antidiabetic Agents Studies
- Magnesium in Health and Disease
- Ion channel regulation and function
- Advanced Glycation End Products research
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- Receptor Mechanisms and Signaling
- Microbial infections and disease research
- Pluripotent Stem Cells Research
- Pneumonia and Respiratory Infections
- Biochemical effects in animals
- Neuroendocrine regulation and behavior
- Sperm and Testicular Function
- Phytochemicals and Antioxidant Activities
- Advanced MRI Techniques and Applications
- Quinazolinone synthesis and applications
- Cardiovascular and exercise physiology
- Antibiotics Pharmacokinetics and Efficacy
- Bioactive Compounds and Antitumor Agents
- Immune Response and Inflammation
- Biochemical Analysis and Sensing Techniques
- Cassava research and cyanide
- Plant Stress Responses and Tolerance
Oklahoma State University
2022-2025
Louisiana State University
2012-2021
University of Hawaiʻi at Mānoa
2004-2006
University of Hawaii System
2004-2006
Queen's Medical Center
2004-2006
Iowa State University
1999-2005
University of South Florida
2005
Lovelace Medical Center
1995
University of New Mexico
1994
TRPM4 has recently been described as a calcium-activated nonselective (CAN) cation channel that mediates membrane depolarization. However, the functional importance of in context calcium (Ca2+) signaling and its effect on cellular responses are not known. Here, molecular inhibition endogenous T cells was shown to suppress currents, with profound influence receptor-mediated Ca2+ mobilization. Agonist-mediated oscillations intracellular concentration ([Ca2+]i), which driven by store-operated...
To better understand the role of irisin in humans, we examined effects human primary adipocytes and fresh subcutaneous white adipose tissue (scWAT). Human derived from 28 female donors' scWAT were used to examine on browning mitochondrial respiration, preadipocytes adipogenesis osteogenesis. Cultured fragments perirenal brown fat for investigating signal transduction pathways that mediate irisin's effect by Western blotting detect phosphorylated forms p38, ERK, STAT3 as well uncoupling...
3,5-Bis(trifluoromethyl)pyrazole derivative (BTP2) or <i>N</i>-[4-3, 5-bis(trifluromethyl)pyrazol-1-yl]-4-methyl-1,2,3-thiadiazole-5-carboxamide (YM-58483) is an immunosuppressive compound that potently inhibits both Ca<sup>2+</sup> influx and interleukin-2 (IL-2) production in lymphocytes. We report here BTP2 dosedependently enhances transient receptor potential melastatin 4 (TRPM4), a Ca<sup>2+</sup>-activated nonselective (CAN) cation channel decreases by depolarizing The effect of on...
Exercise offers short-term and long-term health benefits, including an increased metabolic rate energy expenditure in myocardium. The newly-discovered exercise-induced myokine, irisin, stimulates conversion of white into brown adipocytes as well mitochondrial biogenesis expenditure. Remarkably, irisin is highly expressed myocardium, but its physiological effects the heart are unknown. objective this work to investigate irisin's potential multifaceted on cardiomyoblasts For purpose, H9C2...
Ferulic acid (FA) is the ubiquitous phytochemical phenolic derivative of cinnamic acid. Experimental studies in diabetic models demonstrate that FA possesses multiple mechanisms action associated with anti-hyperglycemic activity. The mechanism by which prevents diabetes-associated vascular damages remains unknown. aim study was to investigate protective effects on protein glycation, lipid peroxidation, membrane ion pump activity, and phosphatidylserine exposure high glucose-exposed human...
Three dietary monosaccharides, (glucose, fructose, and ribose), have different rates of protein glycation that accelerates the production advanced end-products (AGEs). The present work was conducted to investigate effect ferulic acid (FA) on three monosaccharide-mediated glycations oxidation BSA. Comparing percentage reduction, FA (1–5 mM) reduced level fluorescence AGEs (F-AGEs) Nε-(carboxymethyl) lysine (Nε-CML) in glucose-glycated BSA (F-AGEs = 12.61%–36.49%; Nε-CML 33.61%–66.51%),...
Although various biological activities of Phellinus gilvus (PG) have been reported, the active compounds responsible for these effects are not known. Here, we evaluated activity solvent extracts PG, and found ethyl acetate extract (Fd) to be most fraction, showing a strong DPPH free radical scavenging activity, inhibitory on LPS-induced nitric oxide (NO) production COX-2 mRNA expression in RAW264.7 macrophages. Six major were identified from protocatechualdehyde (PCA) was supposed phenolic...
The transient receptor potential melastatin type 7 channel (TRPM7) is a member of the TRP family ion channels that essential for cell proliferation and viability. Mesenchymal stem cells (MSCs) from bone marrow are source tissue repair due to their ability differentiate into specialized cells. However, role TRPM7 in unknown. In this study, we characterized mouse MSCs using molecular biology, immunocytochemistry, patch clamp. We also investigated function lentiviral vector specific shRNA...
Cyanidin is a natural anthocyanidin present in fruits and vegetables with anti-diabetic properties including stimulation of insulin secretion. However, its mechanism action remains unknown. In this study, we elucidated the mechanisms cyanidin for stimulatory secretion from pancreatic β-cells. Rat β-cells INS-1 were used to investigate effects on secretion, intracellular Ca2+ signaling, gene expression. We detected presence space stimulated increased signals concentration-dependent manner....
Riceberry rice (Oryza sativa L.) is a new pigmented variety of from Thailand. Despite its high anthocyanin content, effect on adipogenesis and adipocyte function remains unexplored. We investigated whether extract (RBE) impacted cell proliferation by examining viability cycle, using preadipocyte 3T3-L1 cells. To test RBE’s formation, cells were cultured in adipogenic medium supplemented with number triglyceride levels quantified. Furthermore, Akt1 phosphorylation along RT-qPCR intracellular...
Cyanidin is the most abundant anthocyanin found in red-purple plants and possesses anti-obesity properties. However, its mechanism of action adipocytes remains unknown. The objective this study was to elucidate how cyanidin inhibits adipocyte formation 3T3-L1 preadipocytes. Cells were cultured adipogenic differentiation medium supplemented with examined for adipogenesis, cell viability, gene expression using Oil Red O staining, MTT assay, RT-qPCR. Real-time Ca2+ imaging analysis performed...
Recently, the mechanisms responsible for anti-glycation activity of cyanidin and its derivatives on inhibition methylglyoxal (MG)-induced protein glycation advanced glycation-end products (AGEs) as well oxidative DNA damage were reported. In this study, we investigated protective effect against MG-induced stress apoptosis in rat INS-1 pancreatic β-cells. Exposure cells to cytotoxic levels MG (500 µM) 12 h caused a significant reduction cell viability. However, pretreatment with alone...
We studied changes in muscle proton (1H) transverse relaxation times (T2) by magnetic resonance imaging during exercise and compared these with alterations metabolism measured phosphorus-31 spectroscopy (31P-MRS). Eleven subjects completed two trials of intermittent incremental forearm wrist flexion requiring 30 contractions/min for 5 min, 7 min recovery between stages, 5-N load increments/stage. Between stages the first trial, T2 images 1H were obtained. Muscle increased from 27.3 +/- 1.1...