A5031027918- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Influenza Virus Research Studies
- Respiratory viral infections research
- Microbial infections and disease research
- T-cell and Retrovirus Studies
- SARS-CoV-2 and COVID-19 Research
- Vector-Borne Animal Diseases
- Herpesvirus Infections and Treatments
- Salmonella and Campylobacter epidemiology
- Plant Virus Research Studies
- Effects of Environmental Stressors on Livestock
- Tissue Engineering and Regenerative Medicine
- Molecular Biology Techniques and Applications
- Musicology and Musical Analysis
- Genomics and Phylogenetic Studies
- Animal health and immunology
South Dakota State University
2015-2025
Iowa State University
2020-2021
University of Minnesota System
2020-2021
Kansas State University
2020-2021
University of Minnesota
2020-2021
Pipestone (United States)
2021
University of California, Davis
2020
Cornell University
2020
National Animal Disease Center
2020
Beltsville Agricultural Research Center
2013
The goal of this study was to evaluate survival important viral pathogens livestock in animal feed ingredients imported daily into the United States under simulated transboundary conditions. Eleven viruses were selected based on global significance and impact industry, including Foot Mouth Disease Virus (FMDV), Classical Swine Fever (CSFV), African (ASFV), Influenza A (IAV-S), Pseudorabies virus (PRV), Nipah (NiV), Porcine Reproductive Respiratory Syndrome (PRRSV), Vesicular (SVDV),...
Genetic sequencing, or DNA using the Sanger technique has become widely used in veterinary diagnostic community. This technology plays a role verification of PCR results and is to provide genetic sequence data needed for phylogenetic analysis, epidemiologic studies, forensic investigations. The Laboratory Technology Committee American Association Veterinary Diagnosticians prepared guidelines sample preparation, submission sequencing facilities instrumentation, quality assessment nucleic acid...
Pestiviruses are some of the most significant pathogens affecting ruminants and swine. Here, we assembled a 11 276 bp contig encoding predicted 3635 aa polyprotein from porcine serum with 68 % pairwise identity to that recently partially characterized Rhinolophus affinis pestivirus (RaPV) approximately 25-28 those other pestiviruses. The virus was provisionally named atypical (APPV). Metagenomic sequencing 182 samples identified four additional APPV-positive samples. Positive originated five...
Since its initial detection in May 2013, porcine epidemic diarrhea virus (PEDV) has spread rapidly throughout the US swine industry. Initially, contaminated feed was proposed as a risk factor for PEDV; however, data were not available to support this theory. Here we provide proof of concept by describing novel means recovering PEDV-contaminated complete material from commercial sites and conducting an vivo experiment prove infectivity.For on-farm PEDV RNA feed, paint rollers used collect...
Senecavirus A (SVA) is an emerging picornavirus that has been associated with vesicular disease and neonatal mortality in swine. Many aspects of SVA infection biology pathogenesis, however, remain unknown. Here the pathogenesis was investigated finishing pigs. Animals were inoculated via oronasal route strain SD15-26 monitored for clinical signs lesions infection. Viraemia assessed serum virus shedding oral nasal secretions faeces by real-time reverse transcriptase quantitative PCR (RT-qPCR)...
Senecavirus A (SVA) is an emerging picornavirus that has been recently associated with increased number of outbreaks vesicular disease and neonatal mortality in swine. Many aspects SVA infection biology epidemiology remain unknown. Here, we present a diagnostic investigation conducted swine herds affected by mortality. Clinical environmental samples were collected from unaffected screened for the presence real-time reverse transcriptase PCR virus isolation. Notably, was detected isolated...
Recent, severe outbreaks of porcine epidemic diarrhea virus (PEDV) in Asia and North America highlight the need for well-validated diagnostic tests identification PEDV infected animals evaluation their immune status to this virus. was first detected U.S. May 2013 spread rapidly across country. Some serological assays have been previously described, but few were readily available Several laboratories quickly developed indirect fluorescent antibody (IFA) detection antibodies swine serum,...
This study describes a model developed to evaluate the transboundary risk of PEDV-contaminated swine feed ingredients and effect two mitigation strategies during simulated transport event from China US. Ingredients imported USA China, including organic & conventional soybeans meal, lysine hydrochloride, D-L methionine, tryptophan, Vitamins A, D E, choline, carriers (rice hulls, corn cobs) grade tetracycline, were inoculated with PEDV. Control ingredients, treatments (ingredients plus liquid...
This project investigates the macroepidemiological aspects of porcine reproductive and respiratory syndrome virus (PRRSV) RNA detection by veterinary diagnostic laboratories (VDLs) for period 2007 through 2018. Standardized submission data PRRSV real-time reverse-transcriptase polymerase chain reaction (RT-qPCR) test results from samples were retrieved four VDLs representing 95% all swine tested in NAHLN US. Anonymized organized at case level using SAS (SAS® Version 9.4, SAS® Institute,...
Contaminated complete feed and porcine plasma are risk factors for PEDV introduction to farms a liquid antimicrobial has been proven useful reducing risk. This study provides information on the survivability of across common swine ingredients in presence or absence antimicrobial.Eighteen commonly included commercial diets were selected, including 3 grain sources (corn, soybean meal (SBM), dried distillers grains with solubles (DDGS)), 5 by-products (spray-dried plasma, purified intestinal...
Porcine reproductive and respiratory syndrome virus (PRRSV) is the most important swine pathogen affecting United States of America (USA), leading to significant economic losses. Despite advances in diagnostic testing, there remains a gap understanding genetic evolution PRRSV, especially tracking emergence novel sequences their spread across different regions production stages. This research addresses this by developing systematic methodology for directly collecting analyzing PRRSV ORF5 from...
Boar studs are continuously monitored for the presence of porcine reproductive and respiratory syndrome virus (PRRSV) by testing different biological samples reverse-transcription polymerase chain reaction (RT-PCR). In most cases, run in pools, even though impact pooling on sensitivity RT-PCR is unknown. The objective this study was to evaluate feasibility using PCR pooled through estimation individually, pools 3 5. Twenty-nine boars were inoculated with a low virulent PRRSV isolate. Serum,...
Porcine reproductive and respiratory syndrome virus (PRRSV) continues to be a major problem in the pork industry worldwide. The limitations of current PRRSV vaccines require development new generation vaccines. One key steps future vaccine is include markers for diagnostic differentiation vaccinated animals from those naturally infected with wild-type virus. Using cDNA infectious clone type 1 PRRSV, this study constructed recombinant green fluorescent protein (GFP)-tagged containing deletion...
Since its initial detection in May 2013, porcine epidemic diarrhea virus (PEDV) has spread rapidly throughout the US swine industry. Recently, contaminated feed was confirmed as a vehicle for PEDV infection of naïve piglets. This research provides vivo data supporting ability liquid antimicrobial product to reduce this risk.Sal CURB® (Kemin Industries, Des Moines, IA, USA) is FDA-approved used control Salmonella contamination poultry and diets. To test effect against PEDV, Sal CURB®-treated...
A novel porcine deltacoronavirus (PDCoV), also known as coronavirus HKU15, was reported in China 2012 and identified the U.S. early 2014. Since then, PDCoV has been a number of states linked with clinical disease including acute diarrhea vomiting absence other identifiable pathogens. just recently disease, few specific antibody-based reagents were available to assist diagnosis limited serological capabilities detect an antibody response this virus. Therefore, overall objective project...
Porcine circoviruses (PCVs), including porcine circovirus 2 (PCV2) and 3 (PCV3), have been associated with clinical syndromes in swine, resulting significant economic losses. To better understand the epidemiology relevance of PCV2 PCV3, this study analyzed a dataset comprising diagnostic data from six veterinary laboratories (VDLs) United States America. The comprised polymerase chain reaction (PCR) test results, sample type, age group for PCV3 submissions 2002-2023. Findings indicated...
The introduction of porcine epidemic diarrhea virus (PEDV) into the naive US swine population in April 2013 resulted significant mortality. high mortality rates observed indicated need to boost herd immunity PEDV. To optimize feedback protocols or other future control measures used increase immunity, a fluorescent focus neutralization (FFN) assay was developed and determine titers neutralizing antibodies sow serum, milk, colostrum samples piglet serum samples. Sow from two farm sites within...
We developed a model to predict the cyclic pattern of porcine reproductive and respiratory syndrome virus (PRRSV) RNA detection by reverse-transcription real-time PCR (RT-rtPCR) from 4 major swine-centric veterinary diagnostic laboratories (VDLs) in United States use historical data forecast upcoming year’s weekly percentage positive submissions issue outbreak signals when was not as expected. Standardized submission test results were used. Historical (2015–2017) composed PCR-positive used...