A5083881504- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Microbial infections and disease research
- Animal Behavior and Welfare Studies
- Viral Infections and Immunology Research
- Respiratory viral infections research
- Vector-Borne Animal Diseases
- Influenza Virus Research Studies
- Plant Virus Research Studies
- Genetic and phenotypic traits in livestock
- Molecular Biology Techniques and Applications
- Bacteriophages and microbial interactions
- Effects of Environmental Stressors on Livestock
- Gut microbiota and health
- Veterinary medicine and infectious diseases
- Infection Control and Ventilation
- Transplantation: Methods and Outcomes
- Plant and Fungal Interactions Research
- Herpesvirus Infections and Treatments
- Reproductive tract infections research
- T-cell and Retrovirus Studies
- Aquaculture disease management and microbiota
- Animal Nutrition and Physiology
Iowa State University
2016-2025
Missouri College
2024
National Animal Disease Center
2023
South Dakota State University
2020-2021
Kansas State University
2020-2021
University of Minnesota
2011-2021
University of Minnesota System
2020-2021
Ames National Laboratory
2019-2020
Zoetis (United States)
2018-2020
Tyson Foods (United States)
2020
ABSTRACT Existing genetic classification systems for porcine reproductive and respiratory syndrome virus type 2 (PRRSV-2), such as restriction fragment length polymorphisms sub-lineages, are unreliable indicators of close relatedness or lack sufficient resolution epidemiological monitoring routinely conducted by veterinarians. Here, we outline a fine-scale system PRRSV-2 variants in the United States. Based on >25,000 U.S. open reading frame 5 (ORF5) sequences, sub-lineages were divided...
Numerous, ongoing outbreaks in Brazilian swine herds have been characterized by vesicular lesions sows and acute losses of neonatal piglets. The complete genome Seneca Valley virus (SVV) was identified fluid sera sows, providing evidence association between SVV disease viraemia affected animals.
A 300-sow farrow-to-finish swine operation in the United States experienced a sudden and severe increase mortality neonatal piglets with high morbidity followed by vesicular lesions on snout feet of adult females males. Affected live were submitted for diagnostic investigation. Samples tested polymerase chain reaction (PCR) negative foot-and-mouth disease virus, porcine delta coronavirus, epidemic diarrhoea rotavirus types A, B C, transmissible gastroenteritis reproductive respiratory...
A standardized system for classifying the porcine reproductive and respiratory syndrome virus (PRRSV) status of swine herds is necessary communication between veterinarians producers. The 2011 classification has been widely adopted by producers worldwide. In 2018, a working group met to revisit make recommendations changes. most significant modification was positive unstable stable breeding herds. Recommended diagnostic protocols promotion each were modified recommended maintain added....
Abstract Background Porcine reproductive and respiratory syndrome virus (PRRSV) causes chronic, economically devastating disease in pigs of all ages. Frequent mutations the viral genome result viruses with immune escape mutants. Irrespective regular vaccination, control PRRSV remains a challenge to swine farmers. In PRRSV-infected pigs, innate cytokine IFN-α is inhibited adaptive arm immunity delayed. To elucidate both cellular responses at very early stages infection, seven weeks old...
We performed a longitudinal field study in swine breeding herd that presented with an outbreak of vesicular disease (VD) was associated increase neonatal mortality. Initially, USDA Foreign Animal Disease (FAD) investigation confirmed the presence Senecavirus A (SVA) and ruled out exotic agents produce lesions, e.g., foot-and-mouth virus others. Subsequently, serum samples, tonsil swabs, feces were collected from sows (n = 22) their piglets 33) beginning 1 week after onset clinical weekly for...
This project investigates the macroepidemiological aspects of porcine reproductive and respiratory syndrome virus (PRRSV) RNA detection by veterinary diagnostic laboratories (VDLs) for period 2007 through 2018. Standardized submission data PRRSV real-time reverse-transcriptase polymerase chain reaction (RT-qPCR) test results from samples were retrieved four VDLs representing 95% all swine tested in NAHLN US. Anonymized organized at case level using SAS (SAS® Version 9.4, SAS® Institute,...
Processing fluids (PF), the serosanguinous fluid recovered from piglet castration and tail docking, were used for porcine reproductive respiratory syndrome virus (PRRSV) infection assessment. samples four breed-to-wean herds compared with standard sampling protocols, demonstrating PRRSV RNA detection in PF at greater frequency than schemes.
Disease management and epidemiological investigations of porcine reproductive respiratory syndrome virus-type 2 (PRRSV-2) often rely on grouping together highly related sequences. In the USA, last five years have seen a major shift within swine industry when classifying PRRSV-2, beginning to move away from RFLP (restriction fragment length polymorphisms)-typing adopting use phylogenetic lineage-based classification. However, lineages sub-lineages are large genetically diverse, making them...
Porcine reproductive and respiratory syndrome virus (PRRSV) remains a major concern for swine health. Isolating PRRSV is essential identifying infectious viruses vaccine formulation. This study evaluated the potential of using tongue fluid (TF) from perinatal piglet mortalities isolation. Four collection protocols were tested: extracting TF fresh tissues phosphate-buffered saline (PBS group), transportation medium (VTM freeze-thawed tissue (freeze-thaw homogenates (homogenate group). Two...
Population-based sampling has improved pathogen monitoring in the US swine industry by increasing sensitivity while reducing costs. Postmortem tongue fluids (TF) have emerged as a practical option for porcine reproductive and respiratory syndrome virus (PRRSV) breeding herds, but limited data exist on optimal storage conditions. This study evaluated PRRSV RNA detection via RT-qPCR TF samples under various times, temperatures, viral loads. Additionally, endogenous reference gene (internal...
Effective disease management strategies are essential for achieving optimal pig performance, ensuring high-quality animal health and welfare, maintaining the economic viability of swine systems. Thus, understanding factors that lead to more or less severe critically important. Porcine circovirus type 2 (PCV2) Lawsonia intracellularis (L. intracellularis) endemic pathogens in U.S., affecting herds with varying degrees subclinical clinical impact on performance. While these common pathogens,...
Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), a major challenge for the swine industry, can be transmitted both vertically horizontally. Common sample types, including processing fluid, serum, family oral neither determine whether PRRSV infection originates or horizontally nor directly reflects sow's status. At around 90 days post live-virus inoculation (LVI), 555 sows were sampled by Tonsil-oral-scrubbing (TOSc) 2 weeks pre-farrowing tested RNA. From these, 59 PRRSV-positive...