A5080445655- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Virus-based gene therapy research
- Composite Structure Analysis and Optimization
- Viral Infections and Immunology Research
- Ultrasonics and Acoustic Wave Propagation
- Mechanical Behavior of Composites
- SARS-CoV-2 detection and testing
- Geophysical Methods and Applications
- Respiratory viral infections research
- Structural Analysis and Optimization
- Influenza Virus Research Studies
- Structural Load-Bearing Analysis
- Numerical methods in engineering
- Composite Material Mechanics
- Fatigue and fracture mechanics
- Plant Virus Research Studies
- Thermography and Photoacoustic Techniques
- Topology Optimization in Engineering
- Vector-Borne Animal Diseases
- Structural Health Monitoring Techniques
- Structural mechanics and materials
- vaccines and immunoinformatics approaches
University of Illinois Urbana-Champaign
1983-2024
Koch Institute for Integrative Cancer Research At MIT
2024
Massachusetts Institute of Technology
2024
Kansas State University
2015-2023
Iowa State University
2017
Arkansas Agricultural Experiment Station
2017
North Carolina State University
1992-2015
National Institute of Aerospace
2015
Karolinska Institutet
1997
Porcine circovirus-associated disease (PCVAD) is clinically manifested by postweaning multisystemic wasting syndrome (PMWS), respiratory and enteric disease, reproductive failure, porcine dermatitis nephropathy (PDNS). circovirus 2 (PCV2) an essential component of PCVAD, although etiologic role in PDNS not well established. Here, a novel circovirus, designated 3 (PCV3), was identified sows that died acutely with PDNS-like clinical signs. The capsid replicase proteins PCV3 are only 37% 55%...
The human-animal-environment interface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important aspect the disease 2019 (COVID-19) pandemic that requires robust One Health-based investigations. Despite this, few reports describe natural infections in animals or directly link them to human using genomic data. In present study, we first cases SARS-CoV-2 infection tigers and lions United States provide epidemiological genetic evidence for human-to-animal transmission...
Pestiviruses are some of the most significant pathogens affecting ruminants and swine. Here, we assembled a 11 276 bp contig encoding predicted 3635 aa polyprotein from porcine serum with 68 % pairwise identity to that recently partially characterized Rhinolophus affinis pestivirus (RaPV) approximately 25-28 those other pestiviruses. The virus was provisionally named atypical (APPV). Metagenomic sequencing 182 samples identified four additional APPV-positive samples. Positive originated five...
This report describes the identification and characterization of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in a Malayan tiger U.S. zoo.
Omicron (B.1.1.529) is the most recent SARS-CoV-2 variant of concern, which emerged in late 2021 and rapidly achieved global predominance by early 2022. In this study, we compared infection dynamics, tissue tropism, pathogenesis pathogenicity D614G (B.1), Delta (B.1.617.2), BA.1.1 variants a highly susceptible feline model infection. Although D614G- Delta-inoculated cats became lethargic showed increased body temperatures between days 1 3 postinfection (pi), Omicron-inoculated remained...
Since first identified in December of 2019, COVID-19 has been quickly spreading to the world few months and cases are still undergoing rapid surge most countries worldwide. The causative agent, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), adapts evolves rapidly nature. With availability 16,092 SARS-CoV-2 full genomes GISAID as 13 May, we removed poor-quality performed mutational profiling analysis for remaining 11,183 viral genomes. Global all sequences single nucleotide...
The time-reversal technique has been successfully used in structural health monitoring (SHM) for quantitative imaging of damage. However, the is very time-consuming when it implemented time domain. In this paper, we study frequency‐wavenumber (f‐k) domain fast real-time multiple damage sites plates using scattered flexural plate waves. Based on Mindlin theory, reversibility dispersive waves an isotropic theoretically investigated f‐k A developed by cross-correlation between back-propagated...
The incursion of African swine fever virus (ASFV) into Eurasia presents a threat to the world’s industry. Highly sensitive and specific diagnostic assays are urgently needed for rapid detection during an outbreak, post-outbreak investigation, disease surveillance. In this study, highly repeatable blocking ELISA (bELISA) was developed using recombinant p30 protein as antigen combined with biotinylated mAb against antibody. Initial test validation included sera from 810 uninfected animals 106...
Abstract We describe the first cases of natural SARS-CoV-2 infection detected in animals United States. In March 2020, four tigers and three lions at Bronx Zoo developed mild respiratory signs. RNA was by rRT-PCR secretions and/or feces from all seven affected animals; viral antibodies were their keepers. isolated or situ hybridization co-localized with cellular damage. Whole genome sequence haplotype network analyses showed infected two different strains, suggesting independent...
The global pandemic of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) poses a significant threat to public health. Besides humans, SARS-CoV-2 can infect several animal species. Highly sensitive and specific diagnostic reagents assays are urgently needed for rapid detection implementation strategies prevention control the infection in animals. In this study, we initially developed panel monoclonal antibodies (mAbs) against nucleocapsid protein. To detect broad spectrum species,...
The J-integral is investigated in discrete atomic systems using molecular mechanics simulations. A method of calculating specified domains developed. Two cases, a semiinfinite crack an infinite domain under the remote K-field deformation and finite length geometry tensile shear prescribed on boundary, are studied two-dimensional graphene sheets values obtained small-strain deformation. comparison with energy release rates Mode I II based continuum theory linear elastic fracture show good...
Persistent infection with oncogenic types of human papillomaviruses (HPV) is the major cause cervical cancer precursor lesions. Cellular immune responses are considered important in elimination HPV infection, but targets not well defined. E1 and E2 proteins form a replicative complex necessary for viral genome maintenance. To investigate whether epitopes or can serve as cytotoxic T-lymphocyte (CTL)-mediated killing, we identified peptides containing leukocyte antigen (HLA)-A*0201 binding...
Atypical porcine pestivirus (APPV), a highly divergent pestivirus, has wide geographical distribution around the world. APPV is known to cause type A-II congenital tremors in newborn piglets. The main objective of this study access prevalence US swine herds utilizing newly developed quantitative real-time RT-PCR assay. Retrospective analysis 1,785 samples revealed 19.0% Midwest over period three years (2016-2018). Among all clinical and field that were positive, 82 (24.19%) also positive for...
Abstract Viral capsid proteins are widely explored for subunit vaccine development but often hampered by their complexity of production and low immunogenicity. Here, we report a simple approach to overcome these challenges combining mRNA technology with protein engineering. Using African swine fever virus (ASFV) P72 penton as models, engineered them into membrane-bound secreted forms, compared immunogenicity the native intracellular form in mice pigs through vaccination. The folded...
In this paper a damage imaging technique using pre-stack migration is developed Lamb (guided) wave propagation in composite structures for multi damages by both numerical simulations and experimental studies. particular, the focuses on study finite number of sensors future practical applications. A laminate with surface-mounted linear piezoelectric ceramic (PZT) disk array illustrated as an example. Two types damages, one straight-crack two simulated circular-shaped delamination damage, have...
While vaccination and therapeutics for prevention/treatment of influenza are available, new strategies needed to combat disease in susceptible populations, particularly young children newborns. Host associated microbiota play an important role modulating the virulence numerous pathogens, including A virus. In this study, we examined microbiome-influenza interactions a neonatal piglet model system. The nasal microbiome newborn piglets was longitudinally sampled before after intranasal...
In this paper, a method to focus flexural Lamb waves local area by mounting elastic metamaterials (EMMs) on the surface of plate is proposed. The EMM consists silicon rubber and lead connected in series bonded vertically an aluminum plate. A simplified effective mass-"spring"-mass model used study frequency-dependent mass density determined with aid numerically based medium method. By making use low locally resonant frequency plate, carefully designed different dimensions attain high...
Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically important pathogens affecting global swine industry. Vaccination still a main strategy for PRRSV control; however, host factors associated with vaccine efficacy remain poorly understood. Growing evidence suggests that mucosa-associated microbiomes may play role in responses to vaccination. In this study, we investigated effects killed on gut microbiome diversity pigs. Fecal microbial communities were...