A5022936552- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Virus-based gene therapy research
- Malaria Research and Control
- Viral Infections and Immunology Research
- SARS-CoV-2 and COVID-19 Research
- Vector-borne infectious diseases
- Animal Disease Management and Epidemiology
- Insect symbiosis and bacterial influences
- Insect-Plant Interactions and Control
- Plant Virus Research Studies
- Vector-Borne Animal Diseases
- Study of Mite Species
- CRISPR and Genetic Engineering
- Gold and Silver Nanoparticles Synthesis and Applications
- Antibiotic Resistance in Bacteria
- SARS-CoV-2 detection and testing
- Influenza Virus Research Studies
- Genomics and Phylogenetic Studies
- Respiratory viral infections research
- Spectroscopy and Quantum Chemical Studies
- Enterobacteriaceae and Cronobacter Research
- Viral Infections and Outbreaks Research
Kansas State University
2015-2025
University of Illinois Urbana-Champaign
1992-2025
Guangzhou Medical University
2025
Jiangxi Agricultural University
2024
First Affiliated Hospital of Anhui Medical University
2024
Anhui Medical University
2019-2024
Qingdao Agricultural University
2024
Xi’an Children’s Hospital
2024
Wannan Medical College
2020-2022
China Resources (China)
2022
On nanotextured noble-metal surfaces, surface-enhanced Raman scattering (SERS) is observed, where enhanced by a factor, G, that frequently about one million, but underlying the factor G broad distribution of local enhancement factors, eta. We have measured this for benzenethiolate molecules on 330-nanometer silver-coated nanosphere lattice using incident light wavelength 532 nanometers. A series laser pulses with increasing electric fields burned away at sites progressively decreasing...
Porcine circovirus-associated disease (PCVAD) is clinically manifested by postweaning multisystemic wasting syndrome (PMWS), respiratory and enteric disease, reproductive failure, porcine dermatitis nephropathy (PDNS). circovirus 2 (PCV2) an essential component of PCVAD, although etiologic role in PDNS not well established. Here, a novel circovirus, designated 3 (PCV3), was identified sows that died acutely with PDNS-like clinical signs. The capsid replicase proteins PCV3 are only 37% 55%...
Culex tarsalis Coquillett females were infected with the NY99 strain of West Nile virus (family Flaviviridae, genus Flavivirus, WNV) and then incubated under constant temperatures 10-30 degrees C. At selected time intervals, transmission was attempted using an in vitro capillary tube assay. The median from imbibing infectious bloodmeal until transmitted WNV (median extrinsic incubation period, EIP50) estimated by probit analysis. By regressing EIP rate (inverse as a function temperature 14...
The human-animal-environment interface of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an important aspect the disease 2019 (COVID-19) pandemic that requires robust One Health-based investigations. Despite this, few reports describe natural infections in animals or directly link them to human using genomic data. In present study, we first cases SARS-CoV-2 infection tigers and lions United States provide epidemiological genetic evidence for human-to-animal transmission...
We generated a library of ~1000 Drosophila stocks in which we inserted construct the intron genes allowing expression GAL4 under control endogenous promoters while arresting transcription with polyadenylation signal 3' GAL4. This allows numerous applications. First, ~90% insertions essential cause severe loss-of-function phenotype, an effective way to mutagenize genes. Interestingly, 12/14 chromosomes engineered through CRISPR do not carry second-site lethal mutations. Second, 26/36 (70%)...
Programmed −1 ribosomal frameshifting (−1 PRF) is a gene-expression mechanism used to express many viral and some cellular genes. In contrast, efficient natural utilization of −2 PRF has not been demonstrated previously in eukaryotic systems. Like all nidoviruses, members the Arteriviridae (a family positive-stranded RNA viruses) their replicase polyproteins pp1a pp1ab from two long ORFs (1a 1b), where synthesis depends on PRF. These are posttranslationally cleaved into at least 13...
We present a quantitative analysis of the electron transfer between single gold nanorods and monolayer graphene under no electrical bias. Using single-particle dark-field scattering photoluminescence spectroscopy to access homogeneous linewidth, we observe broadening surface plasmon resonance for on compared quartz substrate. Because absence spectral shifts, dielectric interactions are not important instead assign damping charge plasmon-generated hot electrons that acts as an efficient...
We previously reported a CRISPR-mediated knock-in strategy into introns of
Abstract The recently identified Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) is the cause of COVID-19 pandemic. How this novel beta-coronavirus virus, and coronaviruses more generally, alter cellular metabolism to support massive production ~30 kB viral genomes subgenomic RNAs remains largely unknown. To gain insights, transcriptional metabolomic analyses are performed 8 hours after SARS-CoV-2 infection, an early timepoint where lifecycle completed but prior overt effects on...
Abstract Background Aerosol transmission of COVID-19 is the subject ongoing policy debate. Characterizing aerosol produced by people with critical to understanding role aerosols in transmission. Objective We investigated presence virus size-fractioned from six patients admitted into mixed acuity wards April 2020. Methods Size-fractionated samples and size distributions were collected positive patients. analyzed for viral RNA, cultured Vero E6 cells. Serial RT-PCR cells indicated where...
The ability of the invading NY99 strain West Nile virus (WNV) to elicit an elevated viremia response in California passerine birds was critical for effective infection Culex mosquitoes. Of bird species tested, Western scrub jays, Aphelocoma coerulescens, produced highest response, followed by house finches, Carpodacus mexicanus, and sparrows, Passer domesticus. Most likely, few mourning, Zenaidura macroura, or common ground, Columbina passerine, doves no quail, Callipepla californica,...
Abstract Culex tarsalis Coquillett females were infected with the NY99 strain of West Nile virus (family Flaviviridae, genus Flavivirus, WNV) and then incubated under constant temperatures 10–30°C. At selected time intervals, transmission was attempted using an in vitro capillary tube assay. The median from imbibing infectious bloodmeal until transmitted WNV (median extrinsic incubation period, EIP50) estimated by probit analysis. By regressing EIP rate (inverse as a function temperature 14...
Porcine reproductive and respiratory syndrome (PRRS) virus nonstructural protein 2 (nsp2) contains a cysteine protease domain at its N terminus, which belongs to the ovarian tumor (OTU) family. In this study, we demonstrated that PRRSV nsp2 OTU antagonizes type I interferon induction by interfering with NF-kappaB signaling pathway. Further analysis revealed possesses ubiquitin-deconjugating activity. This has ability inhibit activation polyubiquitination process of IkappaBalpha, subsequently...
We report on the one-photon photoluminescence of gold nanorods with different aspect ratios. measured and scattering spectra from 82 using single-particle spectroscopy. found that emission closely resemble each other independent nanorod ratio. assign to radiative decay longitudinal surface plasmon generated after fast interconversion excited electron-hole pairs were initially created by 532 nm excitation. The intensity was converted quantum yield approximately exponentially decrease as...
ABSTRACT European-like field isolates of porcine reproductive and respiratory syndrome virus (PRRSV) have recently emerged in North America. The full-length genomic sequence an index isolate characterized 1999, strain EuroPRRSV, served as the reference for further studies evolution epidemiology (type 1) United States. Strain EuroPRRSV shared 90.1 to 100% amino acid identity with prototype European strain, Lelystad, within structural nonstructural open reading frames (ORFs) 95.3% overall...
Pestiviruses are some of the most significant pathogens affecting ruminants and swine. Here, we assembled a 11 276 bp contig encoding predicted 3635 aa polyprotein from porcine serum with 68 % pairwise identity to that recently partially characterized Rhinolophus affinis pestivirus (RaPV) approximately 25-28 those other pestiviruses. The virus was provisionally named atypical (APPV). Metagenomic sequencing 182 samples identified four additional APPV-positive samples. Positive originated five...
West Nile virus (family Flaviviridae, genus Flavivirus, WNV) invaded southern California during 2003, successfully overwintered, amplified to epidemic levels, and then dispersed every county in the state. Although surveillance programs tracked measured these events, mechanisms that allowed efficient overwintering subsequent amplification of WNV have not been elucidated. Our current research provided evidence for three whereby may persisted winters 2003–2004 2004–2005: 1) continued enzootic...
Significance Ribosomes synthesize proteins by translating mRNAs into linear chains of amino acids through the decoding consecutive nucleotide triplets (codons). Specific mRNA signals, however, can stimulate ribosomes to shift an alternative triplet reading frame (ribosomal frameshifting) resulting in translation a different protein. Typically, such signals are regions intramolecular base-pairing which form structures that stall ribosome progress. Here we show frameshifting signal used...
African swine fever virus (ASFV) is a contagious, rapidly spreading, transboundary animal disease and major threat to pork production globally. Although plant-based feed has been identified as potential route for introduction onto farms, little known about the risks ASFV transmission in feed. We aimed determine minimum median infectious doses of Georgia 2007 strain through oral exposure during natural drinking feeding behaviors. The dose liquid was 100 50% tissue culture (TCID50), compared...