A5103438779- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Neurobiology and Insect Physiology Research
- Viral Infectious Diseases and Gene Expression in Insects
- Chromosomal and Genetic Variations
- Genomics and Phylogenetic Studies
- Animal Genetics and Reproduction
- Cellular transport and secretion
- Advanced Image Processing Techniques
- Insect Resistance and Genetics
- International Business and FDI
- Insect symbiosis and bacterial influences
- Silk-based biomaterials and applications
- Image and Signal Denoising Methods
- Developmental Biology and Gene Regulation
- Genomics and Chromatin Dynamics
- Corporate Finance and Governance
- Hydrocarbon exploration and reservoir analysis
- Economic Growth and Development
- Protein Degradation and Inhibitors
- Photoacoustic and Ultrasonic Imaging
- Axon Guidance and Neuronal Signaling
- Invertebrate Immune Response Mechanisms
- Image Enhancement Techniques
- Ubiquitin and proteasome pathways
Zhongnan University of Economics and Law
2023
Yancheng Institute of Technology
2022
Baylor College of Medicine
2000-2019
Howard Hughes Medical Institute
2000-2019
Lawrence Berkeley National Laboratory
2006
Abstract The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each gene by the insertion of a single transposable element. As part this effort, transposons in >30,000 fly strains were localized and analyzed relative predicted structures. Approximately 6300 lines that maximize genomic coverage selected be sent Bloomington Stock Center for public distribution, bringing size BDGP disruption collection 7140 lines. It now includes individual 5362 13,666 currently annotated...
We describe a transgenesis platform for Drosophila melanogaster that integrates three recently developed technologies: conditionally amplifiable bacterial artificial chromosome (BAC), recombineering, and bacteriophage PhiC31-mediated transgenesis. The BAC is maintained at low copy number, facilitating plasmid maintenance but induced to high number isolation. Recombineering allows gap repair mutagenesis in bacteria. Gap efficiently retrieves DNA fragments up 133 kilobases long from P1 or...
Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions which 2854 are inserted in coding introns. They allowed us to create library 400 GFP-tagged genes. We show that 72% internally tagged proteins functional, and more than 90% can be imaged unfixed tissues. Moreover, the mRNAs knocked down by RNAi against GFP (iGFPi), efficiently deGradFP technology. The phenotypes associated with RNA protein knockdown typically correspond severe loss function or null...
Abstract The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These often disrupt gene function directly, allow production new alleles, and have many other applications for analyzing function. Here we describe the addition ∼7600 strains, which were selected from >140,000 additional P or piggyBac element integrations 12,500 newly generated Minos transposon. additions...
We generated a library of ~1000 Drosophila stocks in which we inserted construct the intron genes allowing expression GAL4 under control endogenous promoters while arresting transcription with polyadenylation signal 3' GAL4. This allows numerous applications. First, ~90% insertions essential cause severe loss-of-function phenotype, an effective way to mutagenize genes. Interestingly, 12/14 chromosomes engineered through CRISPR do not carry second-site lethal mutations. Second, 26/36 (70%)...
We previously reported a CRISPR-mediated knock-in strategy into introns of
We generated two new genetic tools to efficiently tag genes in
Abstract To identify novel genes and to isolate tagged mutations in known that are required for the development of peripheral nervous system (PNS), we have screened a collection 2460 strains carrying lethal or semilethal P-element insertions on third chromosome. Monoclonal antibody 22C10 was used as marker visualize embryonic PNS. We identified 109 mutant exhibited reproducible phenotypes Cytological genetic analyses these indicated 87 affect previously genes: tramtrack (n = 18 alleles),...
The zinc-finger transcription factor Senseless is co-expressed with basic helix-loop-helix (bHLH) proneural proteins in Drosophila sensory organ precursors and required for their normal development. High levels of synergize bHLH upregulate target gene expression, whereas low act as a repressor vivo. However, the molecular mechanism this dual role unknown. Here, we show that binds via its core zinc fingers recruited by to enhancers function co-activator. Some point mutations region abolish...
Abstract New oil-based mud systems have been widely adopted in the East China Sea. While these materials demonstrated effectiveness reservoir protection under laboratory conditions, variation invasion depth across different systems, influenced by properties such as porosity, lithology, and fluid type, cannot be accurately replicated lab settings. The capillary forces relative permeability between drilling fluids formation hinder laboratory-based assessment of filtrate cleaning processes....
Abstract In our quest for novel genes required the development of embryonic peripheral nervous system (PNS), we have performed three genetic screens using MAb 22C10 as a marker terminally differentiated neurons. A total 66 essential normal PNS were identified, including 49 genes. To obtain information about molecular nature these genes, decided to complement with screen. From transposon-tagged mutations identified on basis their phenotype in selected 31 P-element strains representing 26...
Abstract With the increase of energu demand oil and gas decrease conventional resources in China, exploration development tight reservoirs has becomes first priority to enlarge reserves. The middle-deep sandstone Huagang Formation Xihu Sag East China Sea belongs category is a typical low-permeability, low-porosity reservoir[Xu,G et al. 2012], with strong water-blocking damage, drill-stem test (DST) results from many wells were showed much lower production than what expected. Conventional...
Single image super-resolution (SR) has long posed a challenge in the field of computer vision. While advent deep learning led to emergence numerous methods aimed at tackling this persistent issue, current methodologies still encounter challenges modeling sequence information, leading limitations effectively capturing global pixel interactions. To tackle and achieve superior SR outcomes, we propose Mamba pixel-wise sequential interaction network (MPSI), enhancing establishment long-range...
Image restoration represents a fundamental challenge in low-level vision, focusing on reconstructing high-quality images from their degraded counterparts. With the rapid advancement of deep learning technologies, transformer-based methods with pyramid structures have advanced field by capturing long-range cross-scale spatial interaction. Despite its popularity, degradation essential features during upsampling process notably compromised performance, resulting suboptimal reconstruction...