A5101930997- CRISPR and Genetic Engineering
- Animal Genetics and Reproduction
- Pain Mechanisms and Treatments
- Plant Gene Expression Analysis
- Natural product bioactivities and synthesis
- Metabolism, Diabetes, and Cancer
- Cancer Treatment and Pharmacology
- Pharmacological Effects of Natural Compounds
- Invertebrate Immune Response Mechanisms
- Heavy Metal Exposure and Toxicity
- Plant Molecular Biology Research
- Cancer, Hypoxia, and Metabolism
- Dermatology and Skin Diseases
- Plant-Microbe Interactions and Immunity
- Viral Infectious Diseases and Gene Expression in Insects
- Spine and Intervertebral Disc Pathology
- Pancreatic function and diabetes
- Iron Metabolism and Disorders
- Cancer-related Molecular Pathways
- Cancer-related molecular mechanisms research
- PI3K/AKT/mTOR signaling in cancer
- NF-κB Signaling Pathways
- Photosynthetic Processes and Mechanisms
- Skin Protection and Aging
- Spinal Hematomas and Complications
Huazhong Agricultural University
2017-2025
Henan Provincial People's Hospital
2011-2023
Zhengzhou University
2016-2023
Beijing Tongren Hospital
2023
Capital Medical University
2023
Peking University First Hospital
2022-2023
Peking University
2022-2023
Nanjing Normal University
2009-2022
University of California, Davis
2021
Harvard University
2011-2020
Abstract To facilitate large-scale functional studies in Drosophila, the Drosophila Transgenic RNAi Project (TRiP) at Harvard Medical School (HMS) was established along with several goals: developing efficient vectors for that work all tissues, generating a genome-scale collection of stocks input from community, distributing lines as they are generated through existing stock centers, validating many possible using RT–qPCR and phenotypic analyses, tools web resources identifying retrieving...
We generated a library of ~1000 Drosophila stocks in which we inserted construct the intron genes allowing expression GAL4 under control endogenous promoters while arresting transcription with polyadenylation signal 3' GAL4. This allows numerous applications. First, ~90% insertions essential cause severe loss-of-function phenotype, an effective way to mutagenize genes. Interestingly, 12/14 chromosomes engineered through CRISPR do not carry second-site lethal mutations. Second, 26/36 (70%)...
Recent studies have established SIRT1 as an important regulator of lipid metabolism, although the mechanism its action at molecular level has not been revealed. Here, we show that knockdown with help small hairpin RNA decreases basal and isoproterenol-stimulated lipolysis in cultured adipocytes. This effect is attributed, least part, to suppression rate-limiting lipolytic enzyme, adipose triglyceride lipase (ATGL), transcription. Mechanistically, controls acetylation status functional...
Abstract Different horticultural types of lettuce exhibit tremendous morphological variation. However, the molecular basis for domestication and divergence among different remains unknown. Here, we report RNA sequencing 240 accessions sampled from major wild relatives, generating 1.1 million single-nucleotide polymorphisms (SNPs). Demographic modeling indicates that there was a single event lettuce. We identify list regions as putative selective sweeps occurred during divergence,...
Combining targeted CRISPR-mediated gene editing with an RNAi-mediated screen identifies candidate drug targets.
AMP-activated protein kinase (AMPK) is a fuel-sensing enzyme that activated when cells experience energy deficiency and conversely suppressed in surfeit of supply. AMPK activation improves insulin sensitivity via multiple mechanisms, among which suppresses mTOR/S6K-mediated negative feedback regulation signaling.In the present study we further investigated mechanism AMPK-regulated signaling. Our results showed 5-aminoimidazole-4-carboxamide-1 ribonucleoside (AICAR) greatly enhanced ability...
The Transgenic RNAi Project (TRiP), a Drosophila melanogaster functional genomics platform at Harvard Medical School, was initiated in 2008 to generate and distribute genome-scale collection of RNA interference (RNAi) fly stocks. To date, it has generated >15,000 As this covers most genes, we have largely transitioned development new resources based on CRISPR technology. Here, present an update our libraries publicly available stocks, focus the TRiP-CRISPR overexpression (TRiP-OE) knockout...
In a developing Drosophila melanogaster embryo, mRNAs have maternal origin, zygotic or both. During the maternal-zygotic transition, products are degraded and gene expression comes under control of genome. To interrogate function that both maternally zygotically expressed, it is common to examine embryonic phenotypes derived from female germline mosaics. Recently, development RNAi vectors based on short hairpin RNAs (shRNAs) effective during oogenesis has provided an alternative producing...
Anthocyanins protect plants from biotic and abiotic stressors provide great health benefits to consumers. In this study, we cloned four genes (Red Lettuce Leaves 1 4: RLL1 RLL4) that contribute colour variations in lettuce. The gene encodes a bHLH transcription factor, 5-bp deletion some cultivars abolishes its function activate the anthocyanin biosynthesis pathway. RLL2 an R2R3-MYB which was derived duplication followed by mutations promoter region. RLL3 R2-MYB down-regulates through...
Significance Recently, a number of approaches have been developed to repurpose the CRISPR/Cas9 system as sequence-specific transcriptional activator for gain-of-function experiments (CRISPR activators, or “CRISPRa”). While multiple CRISPRa strategies characterized in cell culture, little is known about their performance vivo. We present an optimized strategy generating large-scale resource Drosophila and show that this has high success rate generates easily recognizable phenotypes describe...
Abstract Acute myeloid leukemia (AML) is a clinically and genetically heterogeneous clonal disease associated with unmet medical needs. Paralleling the pathology of other cancers, AML tumorigenesis propagation can be ascribed to dysregulated cellular processes, including apoptosis. This function others are regulated by tumor suppressor P53, which plays pivotal role in leukemogenesis. Opposing P53-mediated activities mouse double minute 2 homolog (MDM2), promotes P53 degradation. Because TP53...
Several lines of evidence indicate that microRNAs (miRNAs) modulate tolerance to the analgesic effects morphine via regulation pain-related genes, making dysregulation miRNA levels a clinical target for controlling opioid tolerance. However, precise mechanisms by which miRNAs regulate are unclear. In present study, we noted miR-375 level was downregulated but expression Janus kinase 2 (JAK2) upregulated in mouse dorsal root ganglia (DRG) following chronic treatment. The and JAK2 were...
SUMMARY Variegation, a common phenomenon in plants, can be the result of several genetic, developmental, and physiological factors. Leaves some lettuce cultivars exhibit dramatic red variegation; however, genetic mechanisms underlying this variegation remain unknown. In study, we cloned causal gene for on leaves elucidated molecular mechanisms. Genetic analysis revealed that polymorphism variegated versus uniformly is caused by an “AT” repeat promoter RLL2A encoding MYB transcription factor....
Given the diversity of autophagy targets and regulation, it is important to characterize in various cell types conditions. We used a primary myocyte culture system assay role putative regulators specific context skeletal muscle. By treating cultures with rapamycin (Rap) chloroquine (CQ) we induced an autophagic response, fully suppressible by knockdown core ATG genes. screened D. melanogaster orthologs previously reported mammalian protein-protein interaction network, identifying several...
The tender shoots of Chinese toon are one the most popular vegetables, although chemical basis its edible value is still unknown. Guided by antioxidant and α-glucosidase inhibitory activities, main constituents their function content in buds were studied. Six flavonoid glycosides based on quercetin (1–3) kaempferol (4–6), (7), a methyl gallate (8) with inhibition activities isolated identified using chromatographic spectrometric techniques methods. contents seven flavonoids determined...
Painful peripheral neuropathy is a severe side effect in oxaliplatin therapy that compromises cancer patients' quality of life. However, its underlying pathogenic mechanisms remain largely unknown. Here, we found intraperitoneal consecutive administration significantly increased excitability small diameter dorsal root ganglion neurons and induced thermal hyperalgesia rats. Furthermore, the CX3CL1 expression was after treatment, intrathecal injection neutralizing antibody against markedly...
Lung cancer remains a big threat to human health. Growing evidence has reported the crucial regulatory effect of lncRNAs on NSCLC progression. Nevertheless, detailed function lncRNA MBNL1-AS1 involved in development is poorly known. In our research, we confirmed that was significantly reduced patient tissues and cells. Meanwhile, overexpression obviously repressed A549 H1975 cell proliferation, blocked cycle inhibited migration invasion. Moreover, apoptosis increased by MBNL1-AS1. Then,...
Objective: This study aims to investigate gene expression changes in rat dorsal horns after sciatic nerve injury (SNI).Methods: The GSE18803 microarray data collected from young and adult rats were downloaded GEO. After preprocessing, differentially expressed genes (DEGs) between SNI sham-operated groups selected using Limma package, group, respectively, followed by Venn analysis. Then, enrichment analyses performed for these DEGs DAVID. STRING database was used identify protein–protein...
Abstract Cells require some metals, such as zinc and manganese, but excess levels of these metals can be toxic. As a result, cells have evolved complex mechanisms for maintaining metal homeostasis surviving intoxication. Here, we present the results large-scale functional genomic screen in Drosophila cultured modifiers chloride toxicity, together with transcriptomics data wild-type or genetically zinc-sensitized challenged mild supplementation. Altogether, identified 47 genes which knockdown...