A5049491769- Chromosomal and Genetic Variations
- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Genomics and Phylogenetic Studies
- RNA Research and Splicing
- Genomics and Chromatin Dynamics
- Animal Genetics and Reproduction
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- RNA modifications and cancer
- Telomeres, Telomerase, and Senescence
- Plant Virus Research Studies
- Molecular Biology Techniques and Applications
- Plant Reproductive Biology
- Silk-based biomaterials and applications
- Invertebrate Immune Response Mechanisms
- Insect symbiosis and bacterial influences
- Neurobiology and Insect Physiology Research
- Heat shock proteins research
- Plant Disease Resistance and Genetics
- Plant Diversity and Evolution
- Insect and Pesticide Research
- Genetic and Environmental Crop Studies
- Fern and Epiphyte Biology
- Microtubule and mitosis dynamics
Carnegie Institution for Science
1983-2022
Howard Hughes Medical Institute
2004-2021
Department of Embryology
1982-2021
Carnegie Observatories
1982-2008
Syracuse University
2006
Fred Hutch Cancer Center
1989-2005
Laboratoire de Génétique Cellulaire
1992
Centre National de la Recherche Scientifique
1992
Laboratoire de génétique et biologie cellulaire
1992
University of California, Irvine
1992
Abstract The Berkeley Drosophila Genome Project (BDGP) strives to disrupt each gene by the insertion of a single transposable element. As part this effort, transposons in >30,000 fly strains were localized and analyzed relative predicted structures. Approximately 6300 lines that maximize genomic coverage selected be sent Bloomington Stock Center for public distribution, bringing size BDGP disruption collection 7140 lines. It now includes individual 5362 13,666 currently annotated...
Abstract Metazoan physiology depends on intricate patterns of gene expression that remain poorly known. Using transposon mutagenesis in Drosophila, we constructed a library 7404 protein trap and enhancer lines, the Carnegie collection, to facilitate mapping at single-cell resolution. By sequencing genomic insertion sites, determining splicing downstream enhanced green fluorescent (EGFP) exon, analyzing ovary salivary gland, found 600–900 different genes are trapped our collection. A core set...
Here, we document a collection of ∼7434 MiMIC (Minos Mediated Integration Cassette) insertions which 2854 are inserted in coding introns. They allowed us to create library 400 GFP-tagged genes. We show that 72% internally tagged proteins functional, and more than 90% can be imaged unfixed tissues. Moreover, the mRNAs knocked down by RNAi against GFP (iGFPi), efficiently deGradFP technology. The phenotypes associated with RNA protein knockdown typically correspond severe loss function or null...
Abstract The Drosophila Gene Disruption Project (GDP) has created a public collection of mutant strains containing single transposon insertions associated with different genes. These often disrupt gene function directly, allow production new alleles, and have many other applications for analyzing function. Here we describe the addition ∼7600 strains, which were selected from >140,000 additional P or piggyBac element integrations 12,500 newly generated Minos transposon. additions...
We generated a library of ~1000 Drosophila stocks in which we inserted construct the intron genes allowing expression GAL4 under control endogenous promoters while arresting transcription with polyadenylation signal 3' GAL4. This allows numerous applications. First, ~90% insertions essential cause severe loss-of-function phenotype, an effective way to mutagenize genes. Interestingly, 12/14 chromosomes engineered through CRISPR do not carry second-site lethal mutations. Second, 26/36 (70%)...
The white gene of Drosophila is expressed normally when introduced at many different sites in the genome by P-element-mediated DNA transformation, but abnormally inserted two particular genomic positions. It now demonstrated that mutant expression these cases caused surrounding chromosomal region into which has been inserted. could be moved from positions, where it confers a phenotype, to other positions wild-type phenotype. However, flies one new location have an unusual mosaic
Eight terminally deleted Drosophila melanogaster chromosomes have now been found to be "healed." In each case, the healed chromosome end had acquired sequence from HeT DNA family, a complex family of repeated sequences only in telomeric and pericentric heterochromatin. The were apparently added by transposition events involving no homology. We report that transposed healing these identify novel transposable element, HeT-A, which makes up subset family. Addition HeT-A elements broken ends...
Previously, we described a large collection of
TART, a telomere-associated DNA element from Drosophila, is shown in this paper to have structural homology LINE (long interspersed element)-like retrotransposons and transpose broken chromosome ends. TART was detected by situ hybridization 7 of 10 independent additions end. We found evidence that had transposed the end each two were examined detail. From sequence recently transposed, we infer encodes proteins having significant similarity putative many LINEs. These results support...
The white locus of Drosophila melanogaster is a genetically well-characterized locus, mutations in which alter the degree pattern pigmentation eyes. Using previously cloned DNA segment containing portion mutant allele, we have and characterized 48-kilobase chromosomal region Canton S wild-type strain. We mapped positions, relative to restriction endonuclease cleavage sites, several rearrangement breakpoints that bracket while locus. These results define 14 kilobase contains all sequences...
In previous reports, it was shown that both the concentration and rate of production rRNA mRNA were greater in growing than resting 3T6 fibroblasts. Studies on isolated nuclei indicated ribosomal RNA is apparently controlled at level transcription. contrast, hnRNA, putative precursor mRNA, appeared to be synthesized same cells. This finding unexpected has been tested several ways. this report, we show by an independent method relative compared hnRNA several-fold higher However, kinetics...
Genetic studies of the white locus have shown that it has a distal region where structural mutations occur and proximal regulatory occur. To better understand molecular basis this genetic organization we analyzed transcription. A 2.7-kilobase transcript comprising 0.0005% poly(A)-RNA was detected in RNA prepared from pupae or adults. The structure helps clarify some unusual properties locus. There is small 5' exon separated majority sequences found mature by an intron approximately 2.8...
A novel class of repeated sequences exists in the Drosophila melanogaster genome. Three such repeated-sequence families, 412, copia, and 297, have been studied detail by us (Rubin et al. 1976; Finnegan 1978; Potter 1979; Strobel Dunsmuir 1980; Levis 1980). The 297 elements are 7-kb, 5-kb, 6.5-kb long, respectively. Although nonhomologous nucleotide sequence, they share following properties: (1) Elements from each family occur at approximately 30 widely scattered locations chromosomes D....