A5031074190- Developmental Biology and Gene Regulation
- Neurobiology and Insect Physiology Research
- Cellular Mechanics and Interactions
- Cellular transport and secretion
- Viral Infectious Diseases and Gene Expression in Insects
- Insect and Arachnid Ecology and Behavior
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Genomics and Chromatin Dynamics
- Chromosomal and Genetic Variations
- Virus-based gene therapy research
- Hippo pathway signaling and YAP/TAZ
- Invertebrate Immune Response Mechanisms
- Congenital heart defects research
- Protein Kinase Regulation and GTPase Signaling
- Microtubule and mitosis dynamics
- Physiological and biochemical adaptations
- Cancer-related gene regulation
- Signaling Pathways in Disease
- Axon Guidance and Neuronal Signaling
- Epigenetics and DNA Methylation
- Insect symbiosis and bacterial influences
- Genetics, Aging, and Longevity in Model Organisms
- RNA Research and Splicing
- Genetics and Neurodevelopmental Disorders
- Animal Behavior and Reproduction
Technion – Israel Institute of Technology
2011-2025
Rappaport Family Institute for Research in the Medical Sciences
1999-2022
Génétique et biologie du développement
2016
Institut thématique Génétique, génomique et bioinformatique
2016
Howard Hughes Medical Institute
1994-1998
Baylor College of Medicine
1994-1998
National Yang Ming Chiao Tung University
1998
National Sun Yat-sen University
1998
Institute of Molecular Biology, Academia Sinica
1998
University of Dundee
1998
Mutations in the Drosophila gene pavarotti result formation of abnormally large cells embryonic nervous system. In mitotic cycle 16, pav mutant embryos undergo normal anaphase but then develop an abnormal telophase spindle and fail to undertake cytokinesis. We show that septin Peanut, actin, actin-associated protein Anillin, do not become correctly localized mutants. encodes a kinesin-like protein, PAV-KLP, related mammalian MKLP-1. cellularized embryos, is centrosomes early mitosis, midbody...
Chi-Yun Pai, Tung-Sheng Kuo, Thomas J. Jaw, Estee Kurant, Cheng-Tse Chen, Dmitri A. Bessarab, Adi Salzberg, and Y. Henry Sun Institute of Molecular Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan, Republic China, Life Science, National Yat-Sen University, Kaohsiung, Taiwan; Genetics, Yang-Ming Shipai, Taipei, Unit Rappaport Faculty Medicine, Technion-Israel Technology, Haifa 31096, Israel
ABSTRACT The homeotic genes of the bithorax complex are required, among other things, for establishing patterns sensory organs in embryonic peripheral nervous system (PNS). However, molecular mechanisms by which these affect pattern formation PNS not understood and that function this pathway characterized. Here we report phenotypic analysis one such gene, homothorax (hth; also named dorsotonals). Mutations hth gene seem to alter identity abdominal chordotonal neurons, depend on Abd-A their...
APOL1 harbors C–terminal sequence variants (G1 and G2), which account for much of the increased risk kidney disease in sub–Saharan African ancestry populations. Expression has also been shown to cause injury podocytes other cell types, but underlying mechanisms are not understood. We used Drosophila melanogaster Saccharomyces cerevisiae help clarify these mechanisms. Ubiquitous expression human G1 G2 alleles caused near-complete lethality D. , with no effect G0 nonrisk allele, corresponding...
Abstract The Drosophila embryonic peripheral nervous system (PNS) is an excellent model to study the molecular mechanisms governing neural development. To identify genes controlling PNS development, we screened 2000 lethal P-element insertion strains. of mutant embryos was examined using specific marker MAb 22C10, and 92 strains were retained for further analysis. Genetic cytological analysis these shows that 42 mutations affect previously isolated are known be required development:...
Abstract To identify novel genes and to isolate tagged mutations in known that are required for the development of peripheral nervous system (PNS), we have screened a collection 2460 strains carrying lethal or semilethal P-element insertions on third chromosome. Monoclonal antibody 22C10 was used as marker visualize embryonic PNS. We identified 109 mutant exhibited reproducible phenotypes Cytological genetic analyses these indicated 87 affect previously genes: tramtrack (n = 18 alleles),...
Abstract The promoter of the Drosophila melanogaster Ras2 gene is bidirectional, regulating an additional oriented in opposite polarity. two divergently transcribed genes are only 93 bases apart and deletion analysis proved that common cis-acting elements within this region required for transcriptional activity both genes. We cloned paired with bidirectional isolated cDNAs corresponding to its mRNA. Ras (Rop) encodes a 68 103Mr protein which shares sequence homology members novel...
Abstract The adenovirus (Ad) E4orf4 protein contributes to efficient progression of virus infection. When expressed alone induces p53- and caspase-independent cell-death, which is more effective in cancer cells than normal tissue culture. Cancer selectivity E4orf4-induced cell-death may result from interference with various regulatory pathways that are dependent on, including DNA damage signaling proliferation control. conserved several organisms, yeast, Drosophila , mammalian cells,...
Homothorax (HTH) is a homeobox-containing protein, which plays multiple roles in the development of embryo and adult fly. HTH binds to homeotic cofactor Extradenticle (EXD) translocates it nucleus. Its function within nucleus less clear. It was shown, mainly by vitro studies, that can bind DNA as part ternary HTH/EXD/HOX complexes, but little known about transcription regulating HTH-containing complexes context developing Here we present genetic evidence, from vivo for...
Proprioception requires the transduction of muscle-generated deformations into sensory neuronal impulses. In proprioceptive organs, mechanical coupling between neuron and muscle is mediated by a connective structure composed accessory cells an extracellular matrix (ECM). Here, we use fly chordotonal organ (ChO) to investigate how properties element affect mechanosensing. We show that loss Pericardin, major constituent ChO ECM, alters resulting in short-wavelength buckling upon contraction...
Most of the cells in embryonic peripheral nervous system (PNS) Drosophila are born their final location. One known exception is group lateral chordotonal organs (lch5) whose precursors form a dorsal position, yet mature located PNS cluster. Mutations u-turn (ut) locus perturb localization lch5 neurons and result 'dorsal chordotonals' phenotype. We show that ut allelic to ventral veinless (vvl), also as drifter. VVL, POU-domain transcription factor, has been shown participate development...
The homothorax (hth) gene is involved in multiple aspects of embryonic and adult fly development. It encodes a homeodomain-containing protein the MEIS family was shown to regulate subcellular localization homeotic cofactor Extradenticle (EXD). HTH contains TALE class homeodomain conserved MH domain, which required for its interaction with EXD. In this work, we describe structure hth locus, characterize at molecular level collection mutant alleles hth, discuss correlation between identified...
Significance Expression of the adenovirus protein E4orf4 alone in cultured mammalian cells prompts noncanonical apoptosis that is more efficient oncogene-transformed than normal cells. Here, activity a whole organism ( Drosophila melanogaster ) described, leading to three significant conclusions: i E4orf4-induced cell death an evolutionarily conserved process; ii induces distinctive mode death, differing from well-characterized mechanisms; and iii activates but concomitantly inhibits it,...
S ummary Barley coleoptile epidermes, prepared in special mounts for microscopic examination, were infected with Erysiphe graminis D.C. ex Merat (powdery mildew) and, after 2 d, used experiments which reagents introduced to the exposed underside. Fluorescein, by means of fluorescein diacetate, fluoresced periods over 45 min epidermal cytoplasm and longer Haustoria had developed. Cytoplasmic streaming cells continued 24 h. The duration fluorescence was reduced FCCP, but fusicoccin increased...
Proprioception is the ability to sense motion, or position, of body parts by responding stimuli arising within body. In fruitflies and other insects proprioception provided specialized sensory organs termed chordotonal (ChOs) 2. Like many in Drosophila, ChOs develop twice during life cycle fly. First, larval embryogenesis. Then, adult start imaginal discs continue differentiate metamorphosis. The development embryogenesis has been studied extensively 10,11,13,15,16. centerpiece each ChO a...
The gutfeeling (guf) gene was uncovered in a genetic screen for genes that are required proper development of the embryonic peripheral nervous system. Mutations guf cause defects growth cone guidance and fasciculation loss expression several neuronal markers central systems. is terminal differentiation cells. also affect muscles embryo. In absence or activity, myoblasts formed properly, but myoblast fusion further muscle fibers severely impaired. cloned found to encode 21-kD protein with...